scholarly journals Aluminum Nanoparticles as Substrates for Metal-Enhanced Fluorescence in the Ultraviolet for the Label-Free Detection of Biomolecules

2009 ◽  
Vol 81 (4) ◽  
pp. 1397-1403 ◽  
Author(s):  
Mustafa H. Chowdhury ◽  
Krishanu Ray ◽  
Stephen K. Gray ◽  
James Pond ◽  
Joseph R. Lakowicz
Keyword(s):  
Aluminum Nanoparticles ◽  
Label Free ◽  
Enhanced Fluorescence ◽  
Metal Enhanced Fluorescence ◽  
Label Free Detection ◽  
Detection Of Biomolecules

scholarly journals A close analysis of metal-enhanced fluorescence of tryptophan induced by silver nanoparticles: wavelength emission dependence

2013 ◽  
Vol 11 (1) ◽  
pp. 111-115 ◽  
Author(s):  
Anderson Caires ◽  
Luciano Costa ◽  
Joelson Fernandes
Keyword(s):  
Silver Nanoparticles ◽  
Inner Filter Effect ◽  
Label Free ◽  
Enhanced Fluorescence ◽  
Metal Enhanced Fluorescence ◽  
Promising Alternative ◽  
Close Analysis ◽  
Label Free Detection ◽  
Nanoparticles Concentration ◽  
Wavelength Emission

AbstractIn the last few years, silver nanoparticles have been proposed as a promising alternative for the label-free detection of proteins via metal-enhanced fluorescence. Generally, the aromatic amino acid tryptophan is most frequently used in this type of studies, because the intrinsic fluorescence of proteins is usually dominated by tryptophan emissions. In the present study, we evaluated the fluorescence behavior of tryptophan in the presence of a silver colloid with nanoparticles of 100 nm in diameter. The results showed that a nanoparticles concentration of 32 mg L−1 induced maximum fluorescence enhancement. However, the metal-enhanced fluorescence was dependent on the emission wavelength of tryptophan, and this phenomenon was closely related to the metal surface reabsorption process (inner filter effect), suggesting that the plasmon resonance reabsorption effect should be taken into account in analyses involving protein studies by metal-enhanced fluorescence.

Optimization of the nanofluidic design for label-free detection of biomolecules using a nanowall array

2017 ◽  
Vol 250 ◽  
pp. 39-43 ◽  
Author(s):  
Taiga Ajiri ◽  
Takao Yasui ◽  
Masatoshi Maeki ◽  
Akihiko Ishida ◽  
Hirofumi Tani ◽  
...  
Keyword(s):  
Label Free ◽  
Label Free Detection ◽  
Detection Of Biomolecules

Photonic crystals: A platform for label-free and enhanced fluorescence biomolecular and cellular assays

2008 ◽  
Vol 1133 ◽  
Author(s):  
Brian T. Cunningham ◽  
Leo Chan ◽  
Patrick C. Mathias ◽  
Nikhil Ganesh ◽  
Sherine George ◽  
...  
Keyword(s):  
Photonic Crystal ◽  
Photonic Crystals ◽  
High Throughput Screening ◽  
High Sensitivity ◽  
Excitation Wavelength ◽  
Label Free ◽  
Crystal Surfaces ◽  
Enhanced Fluorescence ◽  
Preferred Direction ◽  
Label Free Detection

Abstract Photonic crystal surfaces represent a class of resonant optical structures that are capable of supporting high intensity electromagnetic standing waves with near-field and far-field properties that can be exploited for high sensitivity detection of biomolecules and cells. While modulation of the resonant wavelength of a photonic crystal by the dielectric permittivity of adsorbed biomaterials enables label-free detection, the resonance can also be tuned to coincide with the excitation wavelength of common fluorescent tags - including organic molecules and semiconductor quantum dots. Photonic crystals are also capable of efficiently channeling fluorescent emission into a preferred direction for enhanced extraction efficiency. Photonic crystals can be designed to support multiple resonant modes that can perform label free detection, enhanced fluorescence excitation, and enhanced fluorescence extraction simultaneously on the same device. Because photonic crystal surfaces may be inexpensively produced over large surface areas by nanoreplica molding processes, they can be incorporated into disposable labware for applications such as pharmaceutical high throughput screening. In this talk, the optical properties of surface photonic crystals will be reviewed and several applications will be described, including results from screening a 200,000-member chemical compound library for inhibitors of protein-DNA interactions, gene expression microarrays, and high sensitivity of protein biomarkers.

scholarly journals Tapered Optical Fiber Sensor for Label-Free Detection of Biomolecules

Sensors ◽  
2011 ◽  
Vol 11 (4) ◽  
pp. 3780-3790 ◽  
Author(s):  
Ye Tian ◽  
Wenhui Wang ◽  
Nan Wu ◽  
Xiaotian Zou ◽  
Xingwei Wang
Keyword(s):  
Optical Fiber ◽  
Optical Fiber Sensor ◽  
Fiber Sensor ◽  
Label Free ◽  
Label Free Detection ◽  
Tapered Optical Fiber ◽  
Detection Of Biomolecules

Label-free detection of biomolecules using a tapered optical fiber sensor

2010 ◽  
Author(s):  
Ye Tian ◽  
Wenhui Wang ◽  
Armand Chery, Jr. ◽  
Nan Wu ◽  
Charles Guthy ◽  
...  
Keyword(s):  
Optical Fiber ◽  
Optical Fiber Sensor ◽  
Fiber Sensor ◽  
Label Free ◽  
Label Free Detection ◽  
Tapered Optical Fiber ◽  
Detection Of Biomolecules

Handheld readout system for field-effect transistor biosensor arrays for label-free detection of biomolecules

2015 ◽  
Vol 212 (6) ◽  
pp. 1313-1319 ◽  
Author(s):  
Thanh Chien Nguyen ◽  
Miriam Schwartz ◽  
Xuan Thang Vu ◽  
Jörg Blinn ◽  
Sven Ingebrandt
Keyword(s):  
Field Effect ◽  
Field Effect Transistor ◽  
Label Free ◽  
Readout System ◽  
Biosensor Arrays ◽  
Label Free Detection ◽  
Effect Transistor ◽  
Detection Of Biomolecules

scholarly journals Noise Immune Dielectric Modulated Dual Trench Transparent Gate Engineered MOSFET as a Label Free Biosensor: Proposal and Investigation

2021 ◽  
Author(s):  
DIPANJAN SEN ◽  
Arpan De ◽  
Bijoy Goswami ◽  
Sharmistha Shee ◽  
Subir Kumar Sarkar
Keyword(s):  
High Frequency ◽  
Label Free ◽  
Threshold Voltage Shift ◽  
Enhanced Sensitivity ◽  
Label Free Detection ◽  
Drain Bias ◽  
Overall Performance ◽  
Detection Of Biomolecules ◽  
Insight Into ◽  
Positively Charged

Abstract In this work, we have examined and proposed a dielectrically modulated biosensor based on the dual trench transparent gate engineered MOSFET (DM DT GE-MOSFET) for label-free detection of biomolecules with enhanced sensitivity and efficiency. Different sensing parameters such as the ION/IOFF, threshold voltage shift have been evaluated to validate the sensing metric for the proposed device. Additionally, the SVth (Vth Sensitivity) has been also analyzed by considering the charged (positive and negative) biomolecules. In addition to this, the RF sensing parameters such as the transconductance gain and cut-off frequency have been also taken into account to provide a better insight into the sensitivity analysis of the proposed device. Furthermore, the linearity, distortion and noise immunity of the device has been evaluated to check the overall performance of the biosensor at high frequency (GHz). Moreover, the results indicate that, the proposed biosensor exhibits a SVth of 0.68 for the positively charged biomolecules at a very low drain bias (0.2 V). Therefore, the proposed device can be used as an alternative to the conventional FET-based biosensors.

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