Continuous-Flow Polymerase Chain Reaction of Single-Copy DNA in Microfluidic Microdroplets

2009 ◽  
Vol 81 (1) ◽  
pp. 302-306 ◽  
Author(s):  
Yolanda Schaerli ◽  
Robert C. Wootton ◽  
Tom Robinson ◽  
Viktor Stein ◽  
Christopher Dunsby ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Continuous Flow ◽  
Single Copy ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Copy Dna ◽  
Single Copy Dna

An extrusion fluidic driving method for continuous-flow polymerase chain reaction on a microfluidic chip

2009 ◽  
Vol 168 (1-2) ◽  
pp. 71-78 ◽  
Author(s):  
Zhang-Run Xu ◽  
Xin Wang ◽  
Xiao-Feng Fan ◽  
Jian-Hua Wang
Keyword(s):  
Polymerase Chain Reaction ◽  
Microfluidic Chip ◽  
Continuous Flow ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Continuous flow polymerase chain reaction using a hybrid PMMA-PC microchip with improved heat tolerance

2008 ◽  
Vol 130 (2) ◽  
pp. 836-841 ◽  
Author(s):  
Yi Sun ◽  
M.V.D. Satyanarayan ◽  
Nam Trung Nguyen ◽  
Yien Chian Kwok
Keyword(s):  
Polymerase Chain Reaction ◽  
Heat Tolerance ◽  
Continuous Flow ◽  
Chain Reaction ◽  
Polymerase Chain

A directed search for DNA sequences tightly linked to cereal cyst nematode resistance genes in Triticum tauschii

Genome ◽  
1994 ◽  
Vol 37 (2) ◽  
pp. 311-319 ◽  
Author(s):  
R. F. Eastwood ◽  
E. S. Lagudah ◽  
R. Appels
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Sequences ◽  
Pcr Analysis ◽  
Rflp Markers ◽  
Chain Reaction ◽  
Triticum Tauschii ◽  
Dna Pools ◽  
Polymerase Chain ◽  
Copy Dna ◽  
Dna Pool

An improved system for identifying DNA sequences linked to a targeted region was developed by fractionating DNA sequences prior to polymerase chain reaction (PCR) analysis. In an attempt to identify DNA markers linked to a strong CCN resistance gene, Ccn-D1, in Triticum tauschii, DNA samples from individuals homozygous for resistance and susceptibility at the Ccn-D1 locus in a segregating progeny were bulked separately to produce "near isogenic" DNA pools. The polymerase chain reaction was employed to generate several DNA amplification products from each of the bulked DNA segregants using 240 random (RAPD) and 4 semirandom (consensus sequences of intron–splice junctions) primers. A DNA polymorphic fragment was apparent between the resistant and susceptible bulks using one of the semirandom primers. Hydroxylapatite chromatography of reannealed DNA (to Cot values > 100) was used to enrich low copy DNA sequences in the bulk DNA segregants (resistant and susceptible DNA pools). PCR analysis on the low copy enriched DNA pool increased the level of polymorphism detected between bulked segregants. One of the RAPD fragments present in only the resistant low copy DNA pool was cloned and mapped to the distal region of the long arm of chromosome 2D. By using the cloned RAPD fragment, csE20-2, to assay an RFLP locus in three independent F2 progenies, complete cosegregation was obtained with the Ccn-D1 locus. Joint segregation analysis from a genome-wide mapping of RFLP markers and a second CCN resistance in T. tauschii, Ccn-D2, showed this locus to be loosely linked to the proximal region of chromosome 2.Key words: gene targeting, low copy sequences, RAPD, RFLP, bulk segregants.

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