Discovery of Dopamine Glucuronide in Rat and Mouse Brain Microdialysis Samples Using Liquid Chromatography Tandem Mass Spectrometry

2009 ◽  
Vol 81 (1) ◽  
pp. 427-434 ◽  
Author(s):  
Päivi Uutela ◽  
Laura Karhu ◽  
Petteri Piepponen ◽  
Mikko Käenmäki ◽  
Raimo A. Ketola ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Mouse Brain ◽  
Brain Microdialysis ◽  
Tandem Mass ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass

Validation and quantification of genomic 5-carboxylcytosine (5caC) in mouse brain tissue by liquid chromatography-tandem mass spectrometry

2016 ◽  
Vol 8 (29) ◽  
pp. 5812-5817 ◽  
Author(s):  
Lijian Zhang ◽  
Zhaojin Li ◽  
Guangzhao Chen ◽  
Qionglin Huang ◽  
Junjie Zhang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Brain Tissue ◽  
Mouse Brain ◽  
Accurate Method ◽  
Tandem Mass ◽  
Validation And Quantification ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass

A simple, sensitive and accurate method was developed for 5-carbocytosine detection by LC-MS/MS and was applied to mouse brain tissue.

scholarly journals Quantification of Neurotransmitters in Mouse Brain Tissue by Using Liquid Chromatography Coupled Electrospray Tandem Mass Spectrometry

2014 ◽  
Vol 2014 ◽  
pp. 1-11 ◽  
Author(s):  
Tae-Hyun Kim ◽  
Juhee Choi ◽  
Hyung-Gun Kim ◽  
Hak Rim Kim
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Mouse Brain ◽  
Tandem Mass ◽  
Internal Standards ◽  
Gaba A ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass

A simple and rapid liquid chromatography tandem mass spectrometry method has been developed for the determination of BH4, DA, 5-HT, NE, EP, Glu, and GABA in mouse brain using epsilon-acetamidocaproic acid and isotopically labeled neurotransmitters as internal standards. Proteins in the samples were precipitated by adding acetonitrile, and then the supernatants were separated by a Sepax Polar-Imidazole (2.1 mm × 100 mm, i.d., 3 μm) column by adding a mixture of 10 mM ammonium formate in acetonitrile/water (75 : 25, v/v, 300 μl/min) for BH4 and DA. To assay 5-HT, NE, EP, Glu, and GABA; a Luna 3 μC18(3.0 mm × 150 mm, i.d., 3 μm) column was used by adding a mixture of 1% formic acid in acetonitrile/water (20 : 80, v/v, 350 μl/min). The total chromatographic run time was 5.5 min. The method was validated for the analysis of samples. The calibration curve was linear between 10 and 2000 ng/g for BH4r2=0.995, 10 and 5000 ng/g for DAr2=0.997, 20 and 10000 ng/g for 5-HTr2=0.994, NEr2=0.993, and EPr2=0.993, and 0.2 and 200 μg/g for Glur2=0.996and GABAr2=0.999in the mouse brain tissues. As stated above, LC-MS/MS results were obtained and established to be a useful tool for the quantitative analysis of BH4, DA, 5-HT, NE, EP, Glu, and GABA in the experimental rodent brain.

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