Electrochemical DNA Biosensor Based on Nanoporous Gold Electrode and Multifunctional Encoded DNA−Au Bio Bar Codes

2008 ◽  
Vol 80 (23) ◽  
pp. 9124-9130 ◽  
Author(s):  
Kongcheng Hu ◽  
Dongxiao Lan ◽  
Xuemei Li ◽  
Shusheng Zhang
Keyword(s):  
Gold Electrode ◽  
Dna Biosensor ◽  
Nanoporous Gold ◽  
Electrochemical Dna Biosensor ◽  
Bar Codes ◽  
Nanoporous Gold Electrode

A Sensitive Nanoporous Gold-Based Electrochemical DNA Biosensor forEscherichia coliDetection

2011 ◽  
Vol 44 (16) ◽  
pp. 2559-2570 ◽  
Author(s):  
Kang Li ◽  
Jingjing Huang ◽  
Guoyue Shi ◽  
Wen Zhang ◽  
Litong Jin
Keyword(s):  
Dna Biosensor ◽  
Nanoporous Gold ◽  
Electrochemical Dna Biosensor

Direct electrochemistry and bioelectrocatalysis of horseradish peroxidase entrapped in a self-supporting nanoporous gold electrode: a new strategy to improve the orientation of immobilized enzymes

2015 ◽  
Vol 7 (16) ◽  
pp. 6686-6694 ◽  
Author(s):  
Lu Lu ◽  
Yanjie Dong ◽  
Junwei Wang ◽  
Qianqian Li ◽  
Xia Wu
Keyword(s):  
Horseradish Peroxidase ◽  
Gold Electrode ◽  
Direct Electrochemistry ◽  
Immobilized Enzymes ◽  
Nanoporous Gold ◽  
New Strategy ◽  
Nanoporous Gold Electrode

In an air/water-stable IL, a self-supporting NPGE was first prepared for improving the orientation and biosensing ability of immobilized HRP.

scholarly journals A DNA Electrochemical Sensor via Terminal Protection of Small-Molecule-Linked DNA for Highly Sensitive Protein Detection

Biosensors ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 451
Author(s):  
Ping Ouyang ◽  
Chenxin Fang ◽  
Jialun Han ◽  
Jingjing Zhang ◽  
Yuxing Yang ◽  
...  
Keyword(s):  
Small Molecule ◽  
Gold Electrode ◽  
Folate Receptor ◽  
Protein Detection ◽  
Disease Diagnosis ◽  
Dna Biosensor ◽  
Target Protein ◽  
Receptor Protein ◽  
Electrochemical Dna Biosensor ◽  
Electrochemical Signal

The qualitative and quantitative determination of marker protein is of great significance in the life sciences and in medicine. Here, we developed an electrochemical DNA biosensor for protein detection based on DNA self-assembly and the terminal protecting effects of small-molecule-linked DNA. This strategy is demonstrated using the small molecule biotin and its receptor protein streptavidin (SA). We immobilized DNA with a designed structure and sequence on the surface of the gold electrode, and we named it M1-Biotin DNA. M1-Biotin DNA selectively combines with SA to generate M1-Biotin-SA DNA and protects M1-Biotin DNA from digestion by EXO III; therefore, M1-Biotin DNA remains intact on the electrode surface. M1-Biotin-SA DNA was modified with methylene blue (MB); the MB reporter molecule is located near the surface of the gold electrode, which generates a substantial electrochemical signal during the detection of SA. Through this strategy, we can exploit the presence or absence of an electrochemical signal to provide qualitative target protein determination as well as the strength of the electrochemical signal to quantitatively analyze the target protein concentration. This strategy has been proven to be used for the quantitative analysis of the interaction between biotin and streptavidin (SA). Under optimal conditions, the detection limit of the proposed biosensor is as low as 18.8 pM, and the linear range is from 0.5 nM to 5 μM, showing high sensitivity. The detection ability of this DNA biosensor in complex serum samples has also been studied. At the same time, we detected the folate receptor (FR) to confirm that this strategy can be used to detect other proteins. Therefore, this electrochemical DNA biosensor provides a sensitive, low-cost, and fast target protein detection platform, which may provide a reliable and powerful tool for early disease diagnosis.

High-Linearity Hydrogen Peroxide Sensor Based on Nanoporous Gold Electrode

2019 ◽  
Vol 166 (10) ◽  
pp. B814-B820 ◽  
Author(s):  
Jinlei Huang ◽  
Xufei Fang ◽  
Xue Liu ◽  
Siyuan Lu ◽  
Shuxin Li ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Gold Electrode ◽  
Nanoporous Gold ◽  
High Linearity ◽  
Hydrogen Peroxide Sensor ◽  
Nanoporous Gold Electrode
Sign in / Sign up

Export Citation Format

Share Document