Online In-Tube Solid-Phase Extraction Using a Nonfunctionalized PVC Tube Coupled with ICPMS for in Vivo Monitoring of Trace Metals in Rat Brain Microdialysates

2008 ◽  
Vol 80 (18) ◽  
pp. 6959-6967 ◽  
Author(s):  
C. K. Su ◽  
T. W. Li ◽  
Y. C. Sun
Keyword(s):  
Trace Metals ◽  
Solid Phase Extraction ◽  
Rat Brain ◽  
Solid Phase ◽  
Phase Extraction ◽  
In Vivo Monitoring

A Rapid and Sensitive Method for the Pharmacokinetic Study of Janumet (Sitagliptin and Metformin) Tablets by LC-MS/MS Coupled with Ion-Pair Solid Phase Extraction

2019 ◽  
Vol 15 (7) ◽  
pp. 776-784
Author(s):  
Xiaonian Han ◽  
Jing Wang ◽  
Jing Huang ◽  
Lirong Peng
Keyword(s):  
Solid Phase Extraction ◽  
Pharmacokinetic Study ◽  
Solid Phase ◽  
Multiple Reaction Monitoring ◽  
Ion Pair ◽  
Phase Extraction ◽  
Plasma Samples ◽  
Ionization Source ◽  
Tandem Mass Spectrometric

Background: As first-line treatments for diabetes, sitagliptin and metformin have been widely prescribed as a combination to enhance the therapeutic effect. Objective: To establish a methodology to simultaneously monitor the two drugs in vivo by a reversedphase Liquid Chromatography-Tandem Mass Spectrometric (LC-MS/MS) method. Methods: The two drugs were extracted from 50 μl human plasma by ion-pair solid phase extraction. The separation of the plasma samples was implemented on an Agilent Zorbax SB-CN column (150×4.6 mm, 5.0 µm). The mobile phase was the mixture (80:20, v/v) of methanol and 5.0 mM ammonium formate in water (pH 4.5). An ion trap spectrometer equipped with an electrospray ionization source was utilized to detect the elution in positive mode. Quantification of the analytes was achieved by Multiple Reaction Monitoring (MRM) using the transitions of m/z 408.3→235.1 for sitagliptin and m/z 130.1→ 60.2 for metformin. Results: Sitagliptin and metformin demonstrated good linearity among the range of 1.00-1000 ng/mL and 5.00-4000 ng/mL. The intra-day and inter-day investigations displayed precisions of ≤ 3.6% and an accuracy range of -7.5% to 6.0% for the two drugs. The mean recovery of the two drugs was 96.0% and 98.5%. Under mandatory storage conditions, both the drugs gave an acceptable stability. The throughput of the assay was found to be more than 100 plasma samples per day ascribed to the run time of 3.0 min for each sample. Conclusion: The developed method was successfully applied to a pharmacokinetic study for a fixeddose tablet formulation containing 50 mg sitagliptin and 500 mg metformin in 12 healthy volunteers.

scholarly journals Synthesis of 8-hydroxyquinoline-Amberlite IRC-50 chelator for solid phase extraction of trace metals from groundwater samples

2020 ◽  
Vol 14 (1) ◽  
pp. 697-708 ◽  
Author(s):  
Tamadur A. Labban ◽  
Khaled M. AlMohaimdi ◽  
Snan AlAhmadi ◽  
Mansour Shaikh Ishaqe ◽  
Awadh O. AlSuhaimi
Keyword(s):  
Trace Metals ◽  
Solid Phase Extraction ◽  
Solid Phase ◽  
Phase Extraction ◽  
Groundwater Samples

Combined solid-phase extraction and 2D LC–MS for characterization of the neuropeptides in rat-brain tissue

2005 ◽  
Vol 382 (3) ◽  
pp. 751-759 ◽  
Author(s):  
Anders Holm ◽  
Espen Storbråten ◽  
Albena Mihailova ◽  
Bartosz Karaszewski ◽  
Elsa Lundanes ◽  
...  
Keyword(s):  
Solid Phase Extraction ◽  
Rat Brain ◽  
Brain Tissue ◽  
Solid Phase ◽  
Phase Extraction ◽  
Rat Brain Tissue

Biochelation cartridge for the solid-phase extraction of trace metals

The Analyst ◽  
1990 ◽  
Vol 115 (5) ◽  
pp. 627 ◽  
Author(s):  
Jeremy D. Glennon ◽  
Supalax Srijaranai
Keyword(s):  
Trace Metals ◽  
Solid Phase Extraction ◽  
Solid Phase ◽  
Phase Extraction
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