scholarly journals Trapping Ring Electrode Cell: A FTICR Mass Spectrometer Cell for Improved Signal-to-Noise and Resolving Power

2008 ◽  
Vol 80 (17) ◽  
pp. 6545-6553 ◽  
Author(s):  
Chad R. Weisbrod ◽  
Nathan K. Kaiser ◽  
Gunnar E. Skulason ◽  
James E. Bruce
Keyword(s):  
Mass Spectrometer ◽  
Ring Electrode ◽  
Resolving Power ◽  
Signal To Noise ◽  
Electrode Cell

scholarly journals Limits for resolving tandem mass tag reporter ions with identical integer mass using phase constrained spectrum deconvolution

2018 ◽  
Author(s):  
Christian D. Kelstrup ◽  
Konstantin Aizikov ◽  
Tanveer S. Batth ◽  
Arne Kreutzman ◽  
Dmitry Grinfeld ◽  
...  
Keyword(s):  
Mass Spectrometer ◽  
Peptide Sequencing ◽  
Mass Resolution ◽  
Resolving Power ◽  
Acquisition Time ◽  
Tandem Mass ◽  
Optimal Method ◽  
Orbitrap Mass Spectrometer ◽  
Mass Resolving Power ◽  
Spectrum Deconvolution

ABSTRACTA popular method for peptide quantification relies on isobaric labeling such as tandem mass tags (TMT) which enables multiplexed proteome analyses. Quantification is achieved by reporter ions generated by fragmentation in a tandem mass spectrometer. However, with higher degrees of multiplexing, the smaller mass differences between the reporter ions increase the mass resolving power requirements. This contrasts with faster peptide sequencing capabilities enabled by lowered mass resolution on Orbitrap instruments. It is therefore important to determine the mass resolution limits for highly multiplexed quantification when maximizing proteome depth. Here we defined the lower boundaries for resolving TMT reporter ions with 0.0063 Da mass differences using an ultra-high-field Orbitrap mass spectrometer. We found the optimal method depends on the relative ratio between closely spaced reporter ions and that 64 ms transient acquisition time provided sufficient resolving power for separating TMT reporter ions with absolute ratio changes up to 16-fold. Furthermore, a 32 ms transient processed with phase-constrained spectrum deconvolution provides >50% more identifications with >99% quantified, but with a slight loss in quantification precision and accuracy. These findings should guide decisions on what Orbitrap resolution settings to use in future proteomics experiments relying on TMT reporter ion quantification with identical integer masses.

Analytical model for the signal-to-noise-ratio of drift tube ion mobility spectrometers

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Ansgar T. Kirk ◽  
Alexander Bohnhorst ◽  
Stefan Zimmermann
Keyword(s):  
Ion Mobility ◽  
Drift Tube ◽  
Signal To Noise Ratio ◽  
Resolving Power ◽  
Operating Point ◽  
Optimum Operating ◽  
Signal To Noise ◽  
Experimental Parameters ◽  
Noise Ratio ◽  
Optimum Operating Point

Abstract While the resolving power of drift tube ion mobility spectrometers has been studied and modelled in detail over the past decades, no comparable model exists for the signal-to-noise-ratio. In this work, we develop an analytical model for the signal-to-noise-ratio of a drift tube ion mobility spectrometer based on the same experimental parameters used for modelling the resolving power. The resulting holistic model agrees well with experimental results and allows simultaneously optimizing both resolving power and signal-to-noise-ratio. Especially, it reveals several unexpected relationships between experimental parameters. First, even though reduced initial ion packet widths result in fewer injected ions and reduced amplifier widths result in more noise, the resulting shift of the optimum operating point when reducing both simultaneously leads to a constant signal-to-noise-ratio. Second, there is no dependence of the signal-to-noise-ratio at the optimum operating point on the drift length, as again the resulting shift of the optimum operating point causes all effects to compensate each other.

scholarly journals Petroleomics via Orbitrap mass spectrometry with resolving power above 1 000 000 at m/z 200

RSC Advances ◽  
2018 ◽  
Vol 8 (11) ◽  
pp. 6183-6191 ◽  
Author(s):  
Eduardo M. Schmidt ◽  
Marcos A. Pudenzi ◽  
Jandyson M. Santos ◽  
Celio F. F. Angolini ◽  
Rosana C. L. Pereira ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Fourier Transform ◽  
Electrospray Ionization ◽  
Mass Spectrometer ◽  
High Field ◽  
Resolving Power ◽  
Fourier Transform Mass Spectrometer ◽  
Orbitrap Mass Spectrometry ◽  
Petroleum Sample ◽  
Sample Characterization

The performance of the high-field MegaOrbitrap Fourier transform mass spectrometer (FT-MS) with electrospray ionization (ESI) was evaluated to perform petroleum sample characterization via classical petroleomics approaches.

scholarly journals Excite-Coupled Trapping Ring Electrode Cell (eTREC): Radial Trapping Field Control, Linearized Excitation, And Improved Detection

2010 ◽  
Vol 82 (14) ◽  
pp. 6281-6286 ◽  
Author(s):  
Chad R. Weisbrod ◽  
Nathan K. Kaiser ◽  
Gunnar E. Skulason ◽  
James E. Bruce
Keyword(s):  
Ring Electrode ◽  
Trapping Field ◽  
Electrode Cell ◽  
Field Control

scholarly journals Signal-to-Noise Ratio and Astronomical Fourier Transform Spectroscopy

1988 ◽  
Vol 132 ◽  
pp. 71-78
Author(s):  
J. P. Maillard
Keyword(s):  
Fourier Transform ◽  
Signal To Noise Ratio ◽  
Resolving Power ◽  
High Resolution Spectroscopy ◽  
Signal To Noise ◽  
Array Detectors ◽  
Noise Ratio ◽  
The Fourier Transform ◽  
Large Telescopes ◽  
Very High

The multiplex properties of the Fourier Transform Spectrometer (FTS) can be considered as disadvantageous with modern detectors and large telescopes, the dominant noise source being no longer in most applications the detector noise. Nevertheless, a FTS offers a gain in information and other instrumental features remain: flexibility in choosing resolving power up to very high values, large throughput, essential in high–resolution spectroscopy with large telescopes, metrologic accuracy, automatic substraction of parasitic background. The signal–to–noise ratio in spectra can also be improved: by limiting the bandwidth with cold filters or even cold dispersers, by matching the instrument to low background foreoptics and high–image quality telescopes. The association with array detectors provides the solution for the FTS to regain its full multiplex advantage.

scholarly journals Laser post-ionisation combined with a high resolving power orbitrap mass spectrometer for enhanced MALDI-MS imaging of lipids

2017 ◽  
Vol 53 (53) ◽  
pp. 7246-7249 ◽  
Author(s):  
S. R. Ellis ◽  
J. Soltwisch ◽  
M. R. L. Paine ◽  
K. Dreisewerd ◽  
R. M. A. Heeren
Keyword(s):  
Mass Spectrometer ◽  
Fold Increase ◽  
Maldi Ms ◽  
Resolving Power ◽  
Coupling Laser ◽  
Ms Imaging ◽  
Orbitrap Mass Spectrometer

Coupling laser post-ionisation with a high resolving power MALDI Orbitrap mass spectrometer has realised an up to ∼100-fold increase in the sensitivity and enhanced the chemical coverage for MALDI-MS imaging of lipids relative to conventional MALDI.

scholarly journals 10 K Ring Electrode Trap—Tandem Mass Spectrometer for Infrared Spectroscopy of Mass Selected Ions

2009 ◽  
Author(s):  
Daniel J. Goebbert ◽  
Gerard Meijer ◽  
Knut R. Asmis ◽  
Tetuso Iguchi ◽  
Kenichi Watanabe
Keyword(s):  
Infrared Spectroscopy ◽  
Mass Spectrometer ◽  
Ring Electrode ◽  
Tandem Mass ◽  
Tandem Mass Spectrometer

NOTE ON THE RESOLVING POWER OF MASS SPECTROMETERS

1952 ◽  
Vol 30 (5) ◽  
pp. 503-511 ◽  
Author(s):  
Larkin Kerwin
Keyword(s):  
Mass Spectrometer ◽  
Beam Width ◽  
Resolving Power ◽  
Homogeneous Field ◽  
Current Interest ◽  
Field Mass ◽  
Mass Spectrometers

In calculating the resolving power of a homogeneous field mass spectrometer, one must consider the dispersion and the beam width of the instrument. The latter is the sum of at least 10 components, which are discussed. Formulae giving the resolving power of three types of spectrometer of current interest are developed, as well as formulae for positioning the exit slits.

Sign in / Sign up

Export Citation Format

Share Document