Relative Quantification of Proteins in Human Cerebrospinal Fluids by MS/MS Using 6-Plex Isobaric Tags

2008 ◽  
Vol 80 (8) ◽  
pp. 2921-2931 ◽  
Author(s):  
Loïc Dayon ◽  
Alexandre Hainard ◽  
Virginie Licker ◽  
Natacha Turck ◽  
Karsten Kuhn ◽  
...  
Keyword(s):  
Relative Quantification ◽  
Isobaric Tags

Proteome of equine oviducal fluid: effects of ovulation and pregnancy

2017 ◽  
Vol 29 (6) ◽  
pp. 1085 ◽  
Author(s):  
Katrien Smits ◽  
Hilde Nelis ◽  
Katleen Van Steendam ◽  
Jan Govaere ◽  
Kim Roels ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Large Scale ◽  
Protein Composition ◽  
Absolute Quantification ◽  
Contralateral Side ◽  
Tandem Mass ◽  
Relative Quantification ◽  
Ipsilateral Side ◽  
Isobaric Tags ◽  
In Pregnancy

The equine oviduct plays a pivotal role in providing the optimal microenvironment for early embryonic development, but little is known about the protein composition of the oviducal fluid in the horse. The aim of the present study was to provide a large-scale identification of proteins in equine oviducal fluid and to determine the effects of ovulation and pregnancy. Four days after ovulation, the oviducts ipsilateral and contralateral to the ovulation side were collected from five pregnant and five non-pregnant mares. Identification and relative quantification of proteins in the oviducal fluid of the four groups was achieved by isobaric tags for relative and absolute quantification (iTRAQ) labelling and HPLC–tandem mass spectrometry. The presence of an embryo in the ipsilateral oviducal fluid of pregnant mares induced upregulation of 11 and downregulation of two proteins compared with the contralateral side, and upregulation of 19 proteins compared with the ipsilateral side of non-pregnant mares. Several of these upregulated proteins are related to early pregnancy in other species. The present study represents the first high-throughput identification of proteins in the oviducal fluid of the mare. The results support the hypothesis that the equine embryo interacts with the oviduct, affecting the maternal secretion pattern of proteins involved in pregnancy-related pathways.

scholarly journals Targeted MultiNotch MS3 Approach for Relative Quantification of N-Glycans Using Multiplexed Carbonyl-Reactive Isobaric Tags

2017 ◽  
Vol 90 (2) ◽  
pp. 1129-1135 ◽  
Author(s):  
Bingming Chen ◽  
Xuefei Zhong ◽  
Yu Feng ◽  
Sergei Snovida ◽  
Meng Xu ◽  
...  
Keyword(s):  
Relative Quantification ◽  
Isobaric Tags

A Comparative Study on the Incidence, Aggravation, and Remission of Lupus Nephritis Based on iTRAQ Technology

2020 ◽  
Vol 23 (7) ◽  
pp. 649-657
Author(s):  
Dong-Jiang Liao ◽  
Xi-Ping Cheng ◽  
Nan Li ◽  
Kang-Li Liang ◽  
Hui Fan ◽  
...  
Keyword(s):  
Lupus Nephritis ◽  
Lupus Erythematosus ◽  
Kidney Tissue ◽  
Quantitative Information ◽  
Enzyme Linked Immunosorbent Assay ◽  
Absolute Quantitation ◽  
Western Blot Method ◽  
Close Relationship ◽  
Polymerase Chain ◽  
Isobaric Tags

Aim and Objective: Lupus nephritis (LN) is one of the major complications of systemic lupus erythematosus (SLE). The specific mechanisms of pathogenesis, aggravation, and remission processes in LN have not been clarified but is of great need in the clinic. Using isobaric tags for relative and absolute quantitation (iTRAQ) technology to screen the functional proteins of LN in mice. Especially under intervention factors of lipopolysaccharide (LPS) and dexamethasone. Methods: Mrl-lps mice were intervened with LPS, dexamethasone, and normal saline (NS) using intraperitoneal injection, and c57 mice intervened with NS as control. The anti-ANA antibody enzyme-linked immunosorbent assay (ELISA) was used to verify disease severity. Kidney tissue is collected and processed for iTRAQ to screen out functional proteins closely related to the onset and development of LN. Western blot method and rt-PCR (real-time Polymerase Chain Reaction) were used for verification. Results: We identified 136 proteins that marked quantitative information. Among them, Hp, Igkv8-27, Itgb2, Got2, and Pcx proteins showed significant abnormal manifestations. Conclusion: Using iTRAQ methods, the functional proteins Hp, Igkv8-27, Itgb2, Got2, and Pcx were screened out for a close relationship with the pathogenesis and development of LN, which is worth further study.

scholarly journals Both absolute and relative quantification of urinary mRNA are useful for non-invasive diagnosis of acute kidney allograft rejection

PLoS ONE ◽  
2017 ◽  
Vol 12 (6) ◽  
pp. e0180045 ◽  
Author(s):  
Jung-Woo Seo ◽  
Haena Moon ◽  
Se-Yun Kim ◽  
Ju-Young Moon ◽  
Kyung Hwan Jeong ◽  
...  
Keyword(s):  
Allograft Rejection ◽  
Relative Quantification ◽  
Kidney Allograft ◽  
Non Invasive ◽  
Kidney Allograft Rejection

Combining low- and high-energy tandem mass spectra for optimized peptide quantification with isobaric tags

2010 ◽  
Vol 73 (4) ◽  
pp. 769-777 ◽  
Author(s):  
Loïc Dayon ◽  
Carla Pasquarello ◽  
Christine Hoogland ◽  
Jean-Charles Sanchez ◽  
Alexander Scherl
Keyword(s):  
Mass Spectra ◽  
High Energy ◽  
Tandem Mass ◽  
Peptide Quantification ◽  
Tandem Mass Spectra ◽  
Isobaric Tags
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