Conjugated Polyelectrolyte Based Real-Time Fluorescence Assay for Phospholipase C

2008 ◽  
Vol 80 (1) ◽  
pp. 150-158 ◽  
Author(s):  
Yan Liu ◽  
Katsu Ogawa ◽  
Kirk S. Schanze
Keyword(s):  
Phospholipase C ◽  
Real Time ◽  
Fluorescence Assay ◽  
Conjugated Polyelectrolyte

Carbon Quantum Dots-Based Recyclable Real-Time Fluorescence Assay for Alkaline Phosphatase with Adenosine Triphosphate as Substrate

2015 ◽  
Vol 87 (5) ◽  
pp. 2966-2973 ◽  
Author(s):  
Zhaosheng Qian ◽  
Lujing Chai ◽  
Cong Tang ◽  
Yuanyuan Huang ◽  
Jianrong Chen ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Alkaline Phosphatase ◽  
Real Time ◽  
Adenosine Triphosphate ◽  
Carbon Quantum Dots ◽  
Fluorescence Assay

scholarly journals Mechanism of Coordination of the Bacteriophage T4 DNA Packaging Motor Analyzed by Real-Time Single Molecule Fluorescence Assay

2016 ◽  
Vol 110 (3) ◽  
pp. 46a ◽  
Author(s):  
Li Dai ◽  
Digvijay Singh ◽  
Reza Vafabakhsh ◽  
Marthandan Mahalingam ◽  
Vishal Kottadiel ◽  
...  
Keyword(s):  
Real Time ◽  
Single Molecule ◽  
Bacteriophage T4 ◽  
Dna Packaging ◽  
Fluorescence Assay ◽  
Single Molecule Fluorescence ◽  
Dna Packaging Motor

A real-time fluorescence assay for CPSF73, the nuclease for pre-mRNA 3'-end processing

RNA ◽  
2021 ◽  
pp. rna.078764.121
Author(s):  
Pedro A. Gutierrez ◽  
Kirk Baughman ◽  
Yadong Sun ◽  
Liang Tong
Keyword(s):  
Real Time ◽  
Fluorescence Assay

Real-time Off/On-mode Fluorescence Assay for Enzyme Reactions Involving Nucleoside Polyphosphates by Use of a Xanthene ZnII–Dpa Chemosensor

2008 ◽  
Vol 37 (11) ◽  
pp. 1164-1165 ◽  
Author(s):  
Takahiro Kohira ◽  
Ippei Takashima ◽  
Hiroshi Nonaka ◽  
Akio Ojida ◽  
Itaru Hamachi
Keyword(s):  
Real Time ◽  
Fluorescence Assay ◽  
Enzyme Reactions

scholarly journals Analyzing DNA Packaging Initiation of Bacteriophage T4 by a Real-Time Single Molecule Fluorescence Assay

2012 ◽  
Vol 102 (3) ◽  
pp. 643a
Author(s):  
Reza Vafabakhsh ◽  
Kiran Kondabagil ◽  
Li Dai ◽  
Zhihong Zhang ◽  
Venigalla B. Rao ◽  
...  
Keyword(s):  
Real Time ◽  
Single Molecule ◽  
Bacteriophage T4 ◽  
Dna Packaging ◽  
Fluorescence Assay ◽  
Single Molecule Fluorescence

Phosphoinositide-Phospholipase C β1 Mono-Allelic Deletion Is Associated With Myelodysplastic Syndromes Evolution Into Acute Myeloid Leukemia

2009 ◽  
Vol 27 (5) ◽  
pp. 782-790 ◽  
Author(s):  
Matilde Y. Follo ◽  
Carlo Finelli ◽  
Cristina Clissa ◽  
Sara Mongiorgi ◽  
Costanza Bosi ◽  
...  
Keyword(s):  
Acute Myeloid Leukemia ◽  
Clinical Outcome ◽  
Protein Expression ◽  
Phospholipase C ◽  
Real Time ◽  
Myelodysplastic Syndromes ◽  
Myeloid Leukemia ◽  
Gene And Protein Expression ◽  
Allelic Deletion ◽  
Acute Myeloid

Purpose To evaluate the association between the presence of phosphoinositide-phospholipase C β1 (PI-PLCβ1) mono-allelic deletion with the clinical outcome of myelodysplastic syndromes (MDS) patients. Methods PI-PLCβ1, PI-PLCβ4, and PI-PLCγ1 cytogenetic investigations were performed on 80 newly diagnosed MDS patients (18 low risk, 26 intermediate 1, 18 intermediate 2, 18 high risk) comparing the results with the clinical outcome of the patients. Moreover, fluorescent in situ hybridization results were validated by real-time polymerase chain reaction (PCR). Finally, PI-PLCβ1 gene and protein expression were assessed by both real-time PCR and immunocytochemical experiments. Results Collectively, 35 (43.75%) of 80 of the MDS patients showed a specific mono-allelic deletion of PI-PLCβ1. Kaplan-Meier analysis revealed a significant association (P < .0001) between the PI-PLCβ1 mono-allelic deletion and a higher risk of evolution into acute myeloid leukemia (AML), since 23 of 35 MDS patients (65.7%) bearing the PI-PLCβ1 mono-allelic deletion evolved into AML. Even in multivariate analysis, the PI-PLCβ1 mono-allelic deletion retained a higher significance, with a P < .001, as a prognostic factor of evolution into AML (odds ratio [OR] 1.83; 95% CI, 2.26 to 17.24; P = .00045). Finally, PI-PLCβ1 deletion was related to an altered gene and protein expression. Conclusion PI-PLCβ1 mono-allelic deletion is associated with a worse clinical outcome in MDS patients, hinting at the identification of a new group at higher risk of AML evolution and representing a reliable prognostic tool. Moreover, targeting PI-PLCβ1 pathways might emerge as a new therapeutic strategy for MDS.

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