Time correlated single photon technique: fluorescence lifetime measurements

1976 ◽  
Vol 48 (4) ◽  
pp. 364A-371a ◽  
Author(s):  
L. J. Cline Love ◽  
L. A. Shaver
Keyword(s):  
Fluorescence Lifetime ◽  
Single Photon ◽  
Lifetime Measurements

Fluorescence lifetime measurements under a microscope by the time-correlated single-photon counting technique

1986 ◽  
Vol 35 (5) ◽  
pp. 247-253 ◽  
Author(s):  
Takaaki Minami ◽  
Masaki Kawahigashi ◽  
Yasuhide Sakai ◽  
Katsuyuki Shimamoto ◽  
Satoshi Hirayama
Keyword(s):  
Fluorescence Lifetime ◽  
Single Photon ◽  
Photon Counting ◽  
Lifetime Measurements ◽  
Single Photon Counting ◽  
Counting Technique

scholarly journals Advances in spectroscopic methods for biological crystals. 1. Fluorescence lifetime measurements

2007 ◽  
Vol 40 (6) ◽  
pp. 1105-1112 ◽  
Author(s):  
Antoine Royant ◽  
Philippe Carpentier ◽  
Jérémy Ohana ◽  
John McGeehan ◽  
Bernhard Paetzold ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Single Photon ◽  
Fluorescent Proteins ◽  
Fluorescence Emission ◽  
Spectroscopic Techniques ◽  
Fluorescence Emission Spectrum ◽  
X Ray Diffraction ◽  
Lifetime Measurements ◽  
X Ray ◽  
Solution State

Synchrotrons are now producing thousands of macromolecular structures each year. The need for complementary techniques available on site has progressively emerged, either to assess the relevance of the structure of a protein or to monitor changes that may occur during X-ray diffraction data collection. Microspectrophotometers in the UV–visible absorbance or fluorescence mode have evolved over the past few decades to become the instruments of choice to perform such tests. Described here are recent improvements to the microspectrophotometer of the so-called Cryobench laboratory located at the European Synchrotron Radiation Facility, Grenoble, France. Optical and mechanical properties have been enhanced so as to record better spectra on smaller samples. A device has been implemented to measure the signal decay of fluorescent samples, either in the crystalline or in the solution state. Recording of the fluorescence lifetime in addition to the steady-state fluorescence emission spectrum allows precise monitoring of the fluorescent sample under study. The device consists of an adaptation of a commercially available time-correlated single-photon-counting (TCSPC) system. A method to record and analyze series of TCSPC histograms,e.g.collected as a function of temperature, is described. To validate the instruments, fluorescence lifetimes of fluorescent small molecules or proteins in the crystalline or solution state, at room and cryo temperatures, have been measured. Lifetimes of a number of fluorescent proteins of the GFP family were generally found to be shorter in crystals than in solution, and slightly longer at cryo temperatures than at ambient temperature. The possibility of performing fluorescence lifetime measurements on crystals at synchrotron facilities widens the variety of spectroscopic techniques complementing X-ray diffraction on macromolecular crystallography beamlines.

Fluorescence Lifetime Measurements and Spectral Analysis of Adamantyldiazirine

1997 ◽  
Vol 119 (15) ◽  
pp. 3580-3591 ◽  
Author(s):  
Jeffrey S. Buterbaugh ◽  
John P. Toscano ◽  
William L. Weaver ◽  
James R. Gord ◽  
Christopher M. Hadad ◽  
...  
Keyword(s):  
Spectral Analysis ◽  
Fluorescence Lifetime ◽  
Lifetime Measurements
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