Variable temperature computerized dual-beam, rapid scanning stopped-flow apparatus for the study of air-sensitive systems and transients in enzyme reactions

1975 ◽  
Vol 47 (9) ◽  
pp. 1644-1649 ◽  
Author(s):  
Nicholas. Papadakis ◽  
Richard B. Coolen ◽  
James L. Dye
Keyword(s):  
Variable Temperature ◽  
Stopped Flow ◽  
Enzyme Reactions ◽  
Dual Beam ◽  
Flow Apparatus ◽  
Rapid Scanning

A computer interfaced dual-beam rapid scan stopped-flow system for the study of transients in enzyme reactions

1975 ◽  
Vol 69 (1) ◽  
pp. 155-163 ◽  
Author(s):  
C.H. Suelter ◽  
R.B. Coolen ◽  
N. Papadakis ◽  
J.L. Dye
Keyword(s):  
Flow System ◽  
Stopped Flow ◽  
Enzyme Reactions ◽  
Rapid Scan ◽  
Dual Beam

Stopped Flow Apparatus Employing a Rapid Scanning Monochromator

1966 ◽  
Vol 37 (2) ◽  
pp. 154-157 ◽  
Author(s):  
James L. Dye ◽  
Larry H. Feldman
Keyword(s):  
Stopped Flow ◽  
Flow Apparatus ◽  
Rapid Scanning

High‐pressure stopped‐flow apparatus up to 3 kbar

1979 ◽  
Vol 50 (9) ◽  
pp. 1104-1107 ◽  
Author(s):  
Muneo Sasaki ◽  
Fujitsugu Amita ◽  
Jiro Osugi
Keyword(s):  
High Pressure ◽  
Stopped Flow ◽  
Flow Apparatus

scholarly journals Dual-Channel Stopped-Flow Apparatus for Simultaneous Fluorescence, Anisotropy, and FRET Kinetic Data Acquisition for Binary and Ternary Biological Complexes

Biosensors ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 180
Author(s):  
Roberto F. Delgadillo ◽  
Katie A. Carnes ◽  
Nestor Valles-Villarreal ◽  
Omar Olmos ◽  
Kathia Zaleta-Rivera ◽  
...  
Keyword(s):  
Magic Angle ◽  
Stopped Flow ◽  
Single Shot ◽  
Traditional Methods ◽  
Kinetic Measurements ◽  
Excitation Light ◽  
Dual Channel ◽  
Single Detector ◽  
Flow Apparatus ◽  
Biological Complexes

The Stopped-Flow apparatus (SF) tracks molecular events by mixing the reactants in sub-millisecond regimes. The reaction of intrinsically or extrinsically labeled biomolecules can be monitored by recording the fluorescence, F(t), anisotropy, r(t), polarization, p(t), or FRET, F(t)FRET, traces at nanomolar concentrations. These kinetic measurements are critical to elucidate reaction mechanisms, structural information, and even thermodynamics. In a single detector SF, or L-configuration, the r(t), p(t), and F(t) traces are acquired by switching the orientation of the emission polarizer to collect the IVV and IVH signals however it requires two-shot experiments. In a two-detector SF, or T-configuration, these traces are collected in a single-shot experiment, but it increases the apparatus’ complexity and price. Herein, we present a single-detector dual-channel SF to obtain the F(t) and r(t) traces simultaneously, in which a photo-elastic modulator oscillates by 90° the excitation light plane at a 50 kHz frequency, and the emission signal is processed by a set of electronic filters that split it into the r(t) and F(t) analog signals that are digitized and stored into separated spreadsheets by a custom-tailored instrument control software. We evaluated the association kinetics of binary and ternary biological complexes acquired with our dual-channel SF and the traditional methods; such as a single polarizer at the magic angle to acquire F(t), a set of polarizers to track F(t), and r(t), and by energy transfer quenching, F(t)FRET. Our dual-channel SF economized labeled material and yielded rate constants in excellent agreement with the traditional methods.

scholarly journals Enzyme Kinetics above Denaturation Temperature: A Temperature-Jump/Stopped-Flow Apparatus

2006 ◽  
Vol 91 (12) ◽  
pp. 4605-4610 ◽  
Author(s):  
Bálint Kintses ◽  
Zoltán Simon ◽  
Máté Gyimesi ◽  
Júlia Tóth ◽  
Balázs Jelinek ◽  
...  
Keyword(s):  
Enzyme Kinetics ◽  
Temperature Jump ◽  
Stopped Flow ◽  
Denaturation Temperature ◽  
Flow Apparatus

Stopped-Flow Ultra-Rapid-Scanning Fourier Transform Infrared Spectroscopy on the Millisecond Time Scale

2010 ◽  
Vol 64 (8) ◽  
pp. 907-911 ◽  
Author(s):  
Matthew L. Reback ◽  
Christopher W. Roske ◽  
Thomas E. Bitterwolf ◽  
Peter R. Griffiths ◽  
Christopher J. Manning
Keyword(s):  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Fourier Transform Infrared Spectroscopy ◽  
Time Scale ◽  
Fourier Transform Infrared ◽  
Stopped Flow ◽  
Millisecond Time Scale ◽  
Rapid Scanning
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