Quantitative gas chromatographic determination of ethanolamines as trimethylsilyl derivatives

1975 ◽  
Vol 47 (7) ◽  
pp. 1157-1159 ◽  
Author(s):  
Ryszard. Piekos ◽  
Krzysztof. Kobylczyk ◽  
Janusz. Grzybowski
Keyword(s):  
Chromatographic Determination ◽  
Trimethylsilyl Derivatives

scholarly journals Gas-Chromatographic Determination of the Trimethylsilyl Derivatives of Polyhydric Alcohol Humectants in Tobacco and in Tobacco Smoke

1971 ◽  
Vol 6 (2) ◽  
Author(s):  
N. Carugno ◽  
S. Rossi ◽  
G. Lionetti
Keyword(s):  
Gas Chromatography ◽  
Mass Spectra ◽  
Triethylene Glycol ◽  
Chromatographic Determination ◽  
Flame Ionization ◽  
Trimethylsilyl Derivatives ◽  
Gas Chromatographic Method ◽  
Derivatives Of ◽  
Quantitative Procedure

AbstractA qualitative and quantitative procedure has been developed for the determination of humectants in manufactured tobacco by gas-chromatographic method with a flame ionization detector. It consists of extraction with methanol, concentration of the extract and treatment with Tri-Sil reagents. The operating gas-chromatographic conditions are set forth. Samples of tobacco containing glycerine, propylene glycol, diethylene glycol, triethylene glycol, 1-3 butylene glycol and sorbitol have been analysed with recoveries, for the first five ones, in the range of 95-104 %. In order to verify that each chromatographic peak corresponded to the relative glycol, with no interference by other silylated compounds, the mass spectra were obtained through the combination of gas-chromatography with mass spectrometry. The results achieved confirm, as far as tobacco is concerned, that the procedure is accurate and precise. The same method for the determination of humectants was extended to cigarette smoke. Even though this involves morecomplicated problems, as compared to tobacco, because of the presence of silylated compounds, it was found that, for certain glycols, the gas-chromatography of the trimethyl derivatives can be also used as a method of analysis. The mass spectra of some polyhydric alcohols are shown

THE SEPARATION, DETERMINATION, AND CHARACTERIZATION OF 2-AMINO-2-DEOXY-D-GLUCOSE (D-GLUCOSAMINE) AND 2-AMINO-2-DEOXY-D-GALACTOSE (D-GALACTOSAMINE) IN BIOLOGICAL MATERIALS BY GAS–LIQUID PARTITION CHROMATOGRAPHY

1964 ◽  
Vol 42 (4) ◽  
pp. 451-460 ◽  
Author(s):  
M. B. Perry
Keyword(s):  
Hyaluronic Acid ◽  
Chondroitin Sulphate ◽  
Chromatographic Determination ◽  
Partition Chromatography ◽  
Quantitative Analyses ◽  
Optical Rotations ◽  
Trimethylsilyl Derivatives ◽  
Quantitative Procedure

A method for the separation, determination, and characterization of 2-amino-2-deoxy-D-glucose (D-glucosamine) and 2-amino-2-deoxy-D-galactose (D-galactosamine) is presented. Treatment of 2-acetamido-2-deoxy-α-D-glucose and 2-acetamido-2-deoxy-α-D-galactose in pyridine solution with trimethylchlorosilane and hexamethyldisilazane results in a rapid conversion of the glycoses to their respective trimethylsilyl 3,4,6-tri-O-trimethylsilyl-2-acetamido-2-deoxy-α-D-glycosides which are sufficiently stable and volatile to allow their separation and quantitative analysis to be made by gas–liquid partition chromatography. The two trimethylsilyl derivatives, collected by preparative gas–liquid partition chromatography, were crystalline compounds which had sharp melting points and characteristic infrared spectra and specific optical rotations. Quantitative analyses of mixtures of 2-amino-2-deoxy-D-glucose hydrochloride and 2-amino-2-deoxy-D-galactose hydrochloride were made by gas chromatographic analysis of their trimethylsilyl derivatives formed after prior conversion to their N-acetyl derivatives.The analytical procedure was applied to the characterization of 2-amino-2-deoxy-D-glucose in hyaluronic acid and 2-amino-2-deoxy-D-galactose in chondroitin sulphate. The quantitative procedure was also successfully applied to the analysis of mixtures of hyaluronic acid and chrondroitin sulphate by the gas–liquid partition chromatographic determination of the 2-amino-2-deoxy-D-glucose and 2-amino-2-deoxy-D-galactose in the hydrolyzates prepared from synthetic mixtures of the two mucopolysaccharides.

Gas Chromatographic Determination of cis- and trans-Diethylstilbestrol in Feed Premix

1970 ◽  
Vol 53 (6) ◽  
pp. 1242-1243
Author(s):  
B S Rutherford
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Chromatographic Determination ◽  
Nmr Data ◽  
Trimethylsilyl Derivatives ◽  
Feed Premix ◽  
Cis And Trans

Abstract A method for the determination of the relative amounts of cis- and trans-diethylstilbestrol isomers in feed premix is presented. Trimethylsilyl derivatives are formed and analyzed by gas chromatography. The 2 peaks obtained have been characterized by comparison with NMR data and by mass spectrometry.

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