Quantitative thin-layer chromatography using a flame ionization detector

1970 ◽  
Vol 42 (3) ◽  
pp. 351-354 ◽  
Author(s):  
J. J. Szakasits ◽  
P. V. Peurifoy ◽  
L. A. Woods
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Flame Ionization Detector ◽  
Ionization Detector ◽  
Flame Ionization ◽  
Layer Chromatography

Determination of amniotic fluid phospholipids by thin-layer chromatography, with use of a hydrogen flame ionization detector.

1979 ◽  
Vol 25 (1) ◽  
pp. 31-34 ◽  
Author(s):  
A Martin-Ponthieu ◽  
N Porchet ◽  
J C Fruchart ◽  
G Sezille ◽  
P Dewailly ◽  
...  
Keyword(s):  
Thin Layer ◽  
Amniotic Fluid ◽  
Thin Layer Chromatography ◽  
Internal Standard ◽  
Flame Ionization Detector ◽  
Specific Method ◽  
Ionization Detector ◽  
Flame Ionization ◽  
Hydrogen Flame ◽  
Layer Chromatography

Abstract We describe a new specific method for measuring lecithin, sphingomyelin, and phosphatidylglycerol in amniotic fluid by thin-layer chromatography, with use of a hydrogen flame ionization detector. After extraction and acetone precipitation to isolate surface-active lecithin, the phospholipids are separated on a thin rod of refractory and chemically stable material, having an outer coating of a bonded, sintered partition medium. Two solvent systems are used to develop the chromatograms, chloroform/methanol/water to separate lecithin and sphingomyelin, tetrahydrofuran/methylal/methanol for phosphatidylglycerol. Then the rod is passed through an hydrogen flame; the resulting ions produced generate a current, which is amplified and fed to a potentiometric recorder. The height of integral curves was proportional to the area under each peak. For quantitation we used an internal standard (lysolecithin in the first system. phosphatidyldimethylethanolamine in the second). The method requires less than 5 mL of amniotic fluid; results are available within 6 h.

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