Simultaneous Spectrophotometric Determination of Fructose and Glucose Mixtures by Differential Reaction Rates. Application to Blood Serum Analysis.

1962 ◽  
Vol 34 (11) ◽  
pp. 1443-1446 ◽  
Author(s):  
L. J. Papa ◽  
H. B. Mark ◽  
C. N. Reilley
Keyword(s):  
Blood Serum ◽  
Spectrophotometric Determination ◽  
Reaction Rates ◽  
Serum Analysis ◽  
Differential Reaction

scholarly journals Simultaneous spectrophotometric determination of titanuium (IV) and vanadium (V) using the difference of reaction rates

1972 ◽  
Vol 21 (2) ◽  
pp. 215-220 ◽  
Author(s):  
Yukio NAGAOSA ◽  
Tatsuo YONEKUBO ◽  
Masatada SATAKE ◽  
Rokuzaemon SETO
Keyword(s):  
Spectrophotometric Determination ◽  
Reaction Rates ◽  
The Difference

Spectrophotometric determination of copper in blood serum with 4-(2-quinolylazo)phenol

The Analyst ◽  
1981 ◽  
Vol 106 (1264) ◽  
pp. 799 ◽  
Author(s):  
S. Barua ◽  
Y. S. Varma ◽  
B. S. Garg ◽  
R. P. Singh ◽  
Ishwar Singh
Keyword(s):  
Blood Serum ◽  
Spectrophotometric Determination ◽  
Determination Of Copper

Complexation equilibria between gallium(III) and 4-(4'-methyl-2'-thiazolylazo)-2-methylresorcinol. Derivative spectrophotometric determination of gallium in biological samples

1990 ◽  
Vol 55 (6) ◽  
pp. 1500-1507 ◽  
Author(s):  
Ana I. Jiménez ◽  
Francisco Jiménez ◽  
Juan P. Pérez ◽  
Juan J. Arias
Keyword(s):  
Blood Serum ◽  
Numerical Method ◽  
Stability Constants ◽  
Spectrophotometric Determination ◽  
Human Urine ◽  
Biological Samples ◽  
Second Derivative ◽  
Serum Samples ◽  
The Stability

The reaction of Ga(III) with 4-(4'-methyl-2'-thiazolylazo)-2-methylresorcinol (H2L) at I = 0.25 mol l-1, (NaNO3) was investigated spectrophotometrically. Numerical method was used to evaluate the stability constants of the complexes formed GaH2L2 (log β122 = 37.03±0.09); GaHL (log β111 = 19.08±0.06); GaL (log β101 = 13.65±0.16). A method is developed for the determination of gallium using first and second derivative spectrophotometry and the effect of interferences has been evaluated. The method has been applied to the determination of gallium in human urine and blood serum samples.

Automated enzymic determination of ethanol in blood, serum, and urine with a miniature centrifugal analyzer.

1976 ◽  
Vol 22 (6) ◽  
pp. 802-805 ◽  
Author(s):  
T P Hadjiioannou ◽  
S I Hadjiioannou ◽  
J Avery ◽  
H V Malmstadt
Keyword(s):  
Blood Serum ◽  
Reaction Rates ◽  
Rate Of Change ◽  
Coefficients Of Variation ◽  
Analytical Recovery ◽  
Rate Method ◽  
Relative Errors ◽  
Urine Specimens ◽  
Serum And Urine

Abstract We describe an automated spectrophotometric reaction-rate method for determination of ethanol in serum and urine with a miniature centrifugal analyzer. The theanol is selectively oxidized in the presence of alcochol dehydrogenase and NAD+ to form NADH, which is measured by the rate of change of its absorbance. Reaction rates are determined automatically, and unknown concentrations are calculated from a computer-generated working curve based on aqueous ethanol standards. Blood, serum, or urine specimens need not be deproteinized. The method permits duplicate analysis of at least 30 samples per hour. Coefficients of variation and relative errors are about 2-3% for ethanol concentrations of 0.3-3.0 mug per 2 mul of sample. Analytical recovery of ethanol added to serum is 92-103% (average, 98.5%). Comparisons with distillation-oxidation, gas-chromatographic, and conventional enzymic procedures give satisfactory agreement.

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