Spectrofluorometric Determination of Total Bile Acids in Bile

1961 ◽  
Vol 33 (7) ◽  
pp. 856-860 ◽  
Author(s):  
S. J. Levin ◽  
J. L. Irvin ◽  
C. G. Johnston
Keyword(s):  
Bile Acids ◽  
Spectrofluorometric Determination ◽  
Total Bile Acids

An enzymatic cycling method for the determination of serum total bile acids with recombinant 3α-hydroxysteroid dehydrogenase

2004 ◽  
Vol 326 (1) ◽  
pp. 87-92 ◽  
Author(s):  
Guo-Hua Zhang ◽  
Ai-Ri Cong ◽  
Guo-Bing Xu ◽  
Chuan-Bao Li ◽  
Rui-Feng Yang ◽  
...  
Keyword(s):  
Bile Acids ◽  
Hydroxysteroid Dehydrogenase ◽  
Enzymatic Cycling ◽  
Total Bile Acids

Fluorometric Measurement of Fecal Bile Acids

1968 ◽  
Vol 14 (4) ◽  
pp. 348-359 ◽  
Author(s):  
Donald T Forman ◽  
Charles Phillips ◽  
Walter Eiseman ◽  
C Bruce Taylor
Keyword(s):  
Bile Acids ◽  
Human Subjects ◽  
Mixed Diet ◽  
Fluorometric Method ◽  
Fluorometric Determination ◽  
Fecal Bile Acids ◽  
Normal Human ◽  
Layer Chromatography ◽  
Total Bile Acids

Abstract A method is described for the fluorometric determination of fecal bile acids. The method combines solvent extraction and thin-layer chromatography (TLC) isolation technics with a fluorometric method for measuring total bile acids. Recoveries of individual bile acids added to feces averaged 107% with a standard deviation of ± 5.6%. Bile acid excretion in normal human subjects on a self-selected mixed diet ranged from 196 to 460 mg. total bile acids per 24 hr.

Enzyme inactivation in serum before determination of total bile acids.

1983 ◽  
Vol 29 (6) ◽  
pp. 1073-1075 ◽  
Author(s):  
N Q Hanson ◽  
E F Freier
Keyword(s):  
Bile Acid ◽  
Bile Acids ◽  
Final Concentration ◽  
Methanol Solution ◽  
Enzyme Inactivation ◽  
Total Serum ◽  
Sodium Pyruvate ◽  
Lactate Dehydrogenase Activity ◽  
Total Bile Acids

Abstract Endogenous NADH-generating enzymes must be inactivated before total serum bile acids can be measured accurately by the direct enzymic method. To do this, we pretreat the sera with NaOH, in a final concentration of 0.1 mol/L. Consequently, lactate dehydrogenase activity at least as high as 30 000 U/L is destroyed, obviating blank determinations. Values for bile acid in serum, so obtained, agree with values obtained after pretreatment with heat, an alkali-methanol solution, or sodium pyruvate, but our pretreatment has the advantages of ease, speed, economy, and negligible blank values.

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