Direct Titration of Potassium with Tetraphenylborate. Amperometric Equivalence-Point Detection

1960 ◽  
Vol 32 (10) ◽  
pp. 1253-1257 ◽  
Author(s):  
D. L. Smith ◽  
D. R. Jamieson ◽  
P. J. Elving
Keyword(s):  
Equivalence Point ◽  
Direct Titration ◽  
Point Detection

scholarly journals WEIGHT TITRATION WITH EQUIVALENCE POINT DETECTION BY USING PERSONAL COMPUTER

1991 ◽  
Vol 7 (Supple) ◽  
pp. 753-754 ◽  
Author(s):  
EIKO NAKAMURA ◽  
YUKIO NAGAHARA ◽  
HIROSHI NAMIKI
Keyword(s):  
Personal Computer ◽  
Equivalence Point ◽  
Point Detection

A New Method for Plasminogen Standardization

1968 ◽  
Vol 20 (03/04) ◽  
pp. 548-554
Author(s):  
J Gajewski ◽  
G Markus
Keyword(s):  
Proteolytic Activity ◽  
Specific Activity ◽  
Molar Ratio ◽  
Sharp Transition ◽  
Equivalence Point ◽  
Constant Amount ◽  
Residual Level ◽  
Activity Measurements ◽  
Complete Saturation ◽  
Human Plasminogen

SummaryA method for the standardization of human plasminogen is proposed, based on the stoichiometric interaction between plasminogen and streptokinase, resulting in inhibition of proteolytic activity. Activation of a constant amount of plasminogen with increasing amounts of streptokinase yields linearly decreasing activities, as a function of streptokinase, with a sharp transition to a constant residual level. The point of transition corresponds to complete saturation of plasmin with streptokinase in a 1:1 molar ratio, and is therefore a measure of the amount of plasminogen present initially, in terms of streptokinase equivalents. The equivalence point is independent of the kind of protein substrate used, buffer, pH, length of digestion and, within limits, temperature. The method, therefore, is not subject to the variations commonly encountered in the usual determination based on specific activity measurements.

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