Determination of Free Magnesium Ions in Body Fluids. Improved Methods for Free Calcium Ions, Total Calcium, and Total Magnesium

1960 ◽  
Vol 32 (6) ◽  
pp. 711-717 ◽  
Author(s):  
Mackenzie. Walser
Keyword(s):  
Calcium Ions ◽  
Body Fluids ◽  
Free Calcium ◽  
Magnesium Ions ◽  
Total Calcium ◽  
Free Magnesium ◽  
Improved Methods ◽  
Total Magnesium

Influence of posture on free calcium and related variables.

1979 ◽  
Vol 25 (10) ◽  
pp. 1766-1769 ◽  
Author(s):  
B W Renoe ◽  
J M McDonald ◽  
J H Ladenson
Keyword(s):  
Total Protein ◽  
Hydrogen Ion ◽  
Free Calcium ◽  
Total Calcium ◽  
Healthy Men ◽  
Supine Posture ◽  
Before And After ◽  
The Subject ◽  
Sodium And Potassium ◽  
Total Magnesium

Abstract We measured free calcium and related variables before and after the subject changed from the upright to the supine posture, doing 15 separate such experiments on 11 healthy men. After such a change, free calcium (1.7 +/- 0.4%), total calcium (4.6 +/- 0.7%), total protein (11.5 +/- 1.4%), albumin (12.2 +/- 2.0%), total magnesium (3.8 +/- 0.9%), and the activity of hydrogen ion (2.9 +/- 1.0%) decreased significantly (values are means +/- SEM), but promptly reverted when three subjects assumed the alternative posture. Changes in lactate values were not rapidly reversible; sodium and potassium showed no significant change. The mechanism of the changes in free calcium is unclear, but they correlated only with the changes in total calcium and were notably less than the changes in total calcium, indicating that posture will have less effect on the interpretation of free calcium values than on values for total calcium.

The concentration of free calcium ions and total calcium in pregnancies at term

1982 ◽  
Vol 42 (3) ◽  
pp. 273-277 ◽  
Author(s):  
J. Wandrup ◽  
J. J. Kjer ◽  
J. Thode ◽  
O. Siggaard-Andersen
Keyword(s):  
Calcium Ions ◽  
Free Calcium ◽  
Total Calcium

scholarly journals Simultaneous Determination of Low Free Mg2+and pH in Human Sickle Cells using31P NMR Spectroscopy

2002 ◽  
Vol 277 (51) ◽  
pp. 49911-49920 ◽  
Author(s):  
James P. Willcocks ◽  
Peter J. Mulquiney ◽  
J. Clive Ellory ◽  
Richard L. Veech ◽  
George K. Radda ◽  
...  
Keyword(s):  
Whole Blood ◽  
Blood Cells ◽  
Binding Capacity ◽  
Chemical Shifts ◽  
Spectroscopic Method ◽  
Sickle Cells ◽  
Ph Range ◽  
Free Magnesium ◽  
Total Magnesium

The concentrations of free magnesium, [Mg2+]free, [H+], and [ATP] are important in the dehydration of red blood cells from patients with sickle cell anemia, but they are not easily measured. Consequently, we have developed a rapid, noninvasive NMR spectroscopic method using the phosphorus chemical shifts of ATP and 2,3-diphosphoglycerate (DPG) to determine [Mg2+]freeand pHisimultaneously in fully oxygenated whole blood. The method employs theoretical equations expressing the observed chemical shift as a function of pH, K+, and [Mg2+]free, over a pH range of 5.75–8.5 and [Mg2+]freerange 0–5 mm. The equations were adjusted to allow for the binding of hemoglobin to ATP and DPG, which required knowledge of the intracellular concentrations of ATP, DPG, K+, and hemoglobin. Normal oxygenated whole blood (n= 33) had a pHiof 7.20 ± 0.02, a [Mg2+]freeof 0.41 ± 0.03 mm,and [DPG] of 7.69 ± 0.47 mm. Under the same conditions, whole sickle blood (n= 9) had normal [ATP] but significantly lower pHi(7.10 ± 0.03) and [Mg2+]free(0.32 ± 0.05 mm) than normal red cells, whereas [DPG] (10.8 ± 1.2 mm) was significantly higher. Because total magnesium was normal in sickle cells, the lower [Mg2+]freecould be attributed to increased [DPG] and therefore greater magnesium binding capacity of sickle cells.

scholarly journals Use of a Ca-selective electrode in the determination of total Ca in the production of crude sugar. Preliminary studies

1997 ◽  
Vol 22 (0) ◽  
pp. 193-197
Author(s):  
Jorge GONZÁLEZ GARCÍA ◽  
V.V. EGONOV ◽  
Rubén DEL TÓRO DÉNIZ ◽  
E. DEL TÓRO DÉNIZ ◽  
S. MARTINÉZ SÁEZ ◽  
...  
Keyword(s):  
Sugar Industry ◽  
Free Calcium ◽  
Total Calcium ◽  
Selective Electrode

The determination of the total calcium in juice, syrups, and other products of the sugar industry is investigated. Total calcium and free calcium is determinated by AAS and employing Ca-selective electrode respectively. A coefficient is obtained for the relation of total calcium with respect to free calcium. The coefficient is employed to determine the content of total calcium in accordance with the following equation.<img src="http:/img/fbpe/eq/v22/15e.gif" alt="15e.gif (673 bytes)" align="middle">

Routine Micromethod for Determination of Oxalic Acid in Urine by Atomic Absorption Spectrophotometry

1974 ◽  
Vol 20 (11) ◽  
pp. 1444-1445 ◽  
Author(s):  
R Menachè
Keyword(s):  
Oxalic Acid ◽  
Atomic Absorption ◽  
Calcium Ions ◽  
Atomic Absorption Spectrophotometry ◽  
Total Calcium ◽  
Absorption Spectrophotometry ◽  
The Difference

Abstract The method of Gitelson et al. was modified to a microscale procedure and adapted for atomic absorption spectrophotometry. Oxalic acid in urine is precipitated with excess of calcium ions at pH 5. The excess of calcium present in the supernate and the total calcium added and present in the urine at pH 2 are determined by atomic absorption spectrophotometry. The oxalic acid present in the precipitate is calculated indirectly from the difference between the two determinations of calcium. This method is useful for routine determination of urinary oxalic acid.

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