Determination of Glucose in Blood Serum by New Rapid and Specific Automatic System

1960 ◽  
Vol 32 (3) ◽  
pp. 394-398 ◽  
Author(s):  
H. V. Malmstadt ◽  
G. P. Hicks
Keyword(s):  
1980 ◽  
Vol 45 (4) ◽  
pp. 1099-1108 ◽  
Author(s):  
Mikuláš Chavko ◽  
Michal Bartík ◽  
Evžen Kasafírek

A polarographic study of the hydrolysis of [8-lysine]vasopressin and some hormonogens of the vasopressin series with the blood serum of women in the last week of pregnancy was studied. The dependence of hydrolysis on pH (pH optimum: 7.4-7.50, substrate concentration (Km 1.2 . 10-5M), pH stability and thermal stability were determined. The rate of hydrolysis of individual vasopressin analogues decreases in the order: [8-lysine]vasopressin > Nα-glycyl-prolyl[8-lysine]-vasopressin > Nα-leucyl-[8-lysine]vasopressin > Nα-alanyl-[8-lysine]vasopressin > Nα-phenyl alanyl-[8-lysine]vasopressin > Nα-diglycyl-[8-lysine]vasopressin > Nα-prolyl-[8-lysine]vasopressin > Nα-triglycyl-[8-lysine]vasopressin > Nα-sarcosyl-glycyl-[8-lysine]vasopressin. The degree of hydrolysis gradually increases to a multiple with the length of the pregnancy in consequence of the presence of oxytocine. However, vasopressin is also hydrolysed to a small extent with the enzymes from the blood sera of non-pregnant women. Under similar analytical conditions oxytocin was not hydrolysed with the sera of non-pregnant women and therefore oxytocin is a more suitable substrate than vasopressin for polarographic determination of serum oxytocinase.


1930 ◽  
Vol 87 (1) ◽  
pp. 27-32
Author(s):  
F.H.L. Taylor
Keyword(s):  

1983 ◽  
Vol 81 (1-2) ◽  
pp. 135-147 ◽  
Author(s):  
U. Thanei-Wyss ◽  
W. E. Morf ◽  
P. Lienemann ◽  
Z. Stefanac ◽  
I. Mostert ◽  
...  

2017 ◽  
Vol 35 (2) ◽  
pp. 233-240 ◽  
Author(s):  
Martina Rejtharová ◽  
Libor Rejthar ◽  
Katarína Čačková
Keyword(s):  

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