Ion Exchange Chromatography of Amino Acids. Semiautomatic Method of Operation with Cationic Exchange Resin Columns

1959 ◽  
Vol 31 (9) ◽  
pp. 1504-1512 ◽  
Author(s):  
P. B. Hamilton ◽  
R. A. Anderson
Keyword(s):  
Amino Acids ◽  
Ion Exchange ◽  
Exchange Resin ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Cationic Exchange

The non-protein nitrogen composition of grass silages: I. The estimation of the basic amino acids and non-volatile amines by chromatography on a weak cation exchange resin

1969 ◽  
Vol 72 (3) ◽  
pp. 459-466 ◽  
Author(s):  
A. D. Hughes
Keyword(s):  
Amino Acids ◽  
Ion Exchange ◽  
Cation Exchange ◽  
Exchange Resin ◽  
Cation Exchange Resin ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Protein Nitrogen ◽  
Weak Cation Exchange

SUMMARYInvestigations into the non-protein nitrogen composition of grass silages using the 50 cm strong cation-exchange column of Spackman, Stein & Moore (1958) to determine the basic amino acids led to difficulties in the determination of ethanolamine in the presence of high concentrations of ammonia, and of histidine in the presence of δ amino-n-valeric acid. An alternative technique for the ion exchange chromatography and estimation of histidine, lysine, ornithine, ethanolamine, arginine and ammonia on a weak cation-exchange resin has been developed. This method enables small amounts of ethanolamine to be determined in the presence of large amounts of ammonia and values for the ethanolamine content of a number of silage samples are presented. When used in conjunction with the technique of Spackman et al. (1958) the δ-amino-n-valeric acid content of grass silages could also be determined in the presence of histidine.The estimation of amines produced by the microbial decomposition of herbage proteins during ensiling has previously involved their initial separation from the amino acids followed by quantitative partition chromatography. An alternative method for the estimation of these amines by ion-exchange chromatography on a weak cation-exchange resin is described. This method permits the colorimetric determination of β-phenylethylamine, tyramine, tryptamine, 5-hydroxytryptamine, putrescine, cadaverine and histamine without interference from the amino acids. The efficiency of this technique has been investigated using standard solutions of the naturally occurring amines and samples of good quality and of high pH spoilt silages.

scholarly journals Free amino acids in fruit bodies of some wood destroying fungi

2014 ◽  
Vol 14 (1-2) ◽  
pp. 151-155
Author(s):  
Wiesław Tadeusiak ◽  
Eliza Balicka
Keyword(s):  
Amino Acids ◽  
Ion Exchange ◽  
Free Amino Acids ◽  
Free Amino ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Polyporus Squamosus

Concentration of free amino acids in the following bracket fungi: <i>Climacodon septentrionalis</i> (Fr) P. Karst, <i>Hapalopilus croceus</i> (Pers. ex Fr.) Donk., <i>Laetiporusus sulphurens</i> (Bull. ex Fr.) Murill and <i>Polyporus squamosus</i> Huds ex Fr., were determined by ion-exchange chromatography.

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