Separation of Aureomycin from Terramycin by Countercurrent Distribution and by Paper Chromatography

1954 ◽  
Vol 26 (10) ◽  
pp. 1640-1642 ◽  
Author(s):  
R. J. Hickey ◽  
W. F. Phillips
Keyword(s):  
Paper Chromatography ◽  
Countercurrent Distribution

scholarly journals The inhibition of N-acetyl-β-d-glucosaminase by the (1→4)- and (1→5)-lactones of N-acetylglucosaminic acid

1970 ◽  
Vol 119 (2) ◽  
pp. 303-306 ◽  
Author(s):  
T. E. Couling ◽  
R. Goodey
Keyword(s):  
Paper Chromatography ◽  
Relative Stability ◽  
Quantitative Measurement ◽  
Colorimetric Method ◽  
Countercurrent Distribution

The inhibition of N-acetyl-β-d-glucosaminase activity by 2-acetamido-2-deoxy-d-gluconolactone was examined. Separation of the (1→4)- and (1→5)-lactones was achieved by using paper chromatography or countercurrent distribution and identification was obtained by examination of the relative stability of the components of separated material. Quantitative measurement of the two lactones was by kinetic titration or by a colorimetric method based on their reaction with hydroxylamine. It was shown that only the (1→5)-lactone acted as an inhibitor of N-acetyl-β-d-glucosaminase.

scholarly journals THE PURIFICATION OF HYPERTENSIN I

1954 ◽  
Vol 100 (4) ◽  
pp. 363-370 ◽  
Author(s):  
Leonard T. Skeggs ◽  
Walton H. Marsh ◽  
Joseph R. Kahn ◽  
Norman P. Shumway
Keyword(s):  
Amino Acids ◽  
Nitrogen Content ◽  
Acid Hydrolysis ◽  
Paper Chromatography ◽  
Solid Acid ◽  
Specific Activity ◽  
Single Component ◽  
Countercurrent Distribution ◽  
Pressor Agent

The purification of hypertensin I has been described. The final product which is four times as powerful a pressor agent as l-arterenol, is obtained with an over-all recovery of 40 per cent. The product consists of a single component in countercurrent distribution, having a nitrogen content of 15.97 per cent and a specific activity of 7050 Goldblatt units per mg. of N or 1125 units per mg. of solid. Acid hydrolysis and paper chromatography indicate in a preliminary fashion that there are about nine amino acids present in the intact polypeptide.

Indoles in Uremia: Identification by Countercurrent Distribution and Paper Chromatography

1968 ◽  
Vol 21 (5) ◽  
pp. 436-450 ◽  
Author(s):  
GEORGE D. LUDWIG ◽  
DOROTHY SENESKY ◽  
L. W. BLUEMLE ◽  
J. RUSSELL ELKINTON
Keyword(s):  
Paper Chromatography ◽  
Countercurrent Distribution

SEPARATION OF ENDOMYCINS A AND B, AND THEIR IDENTIFICATION AS MEMBERS OF THE POLYENE GROUPS OF ANTIFUNGAL ANTIBIOTICS

1957 ◽  
Vol 35 (12) ◽  
pp. 1461-1466 ◽  
Author(s):  
L. C. Vining ◽  
W. A. Taber
Keyword(s):  
Absorption Spectra ◽  
Paper Chromatography ◽  
Ultraviolet Absorption ◽  
Countercurrent Distribution ◽  
Double Bonds ◽  
Active Components ◽  
Streptomyces Species ◽  
Antifungal Antibiotics

An unidentified Streptomyces species isolated from platings of "honey dew" of the fungus Clavicepspurpurea produced two antifungal antibiotics which were shown by paper chromatography to be identical with the two active components of endomycin, and also with helixins A and B. An examination of the ultraviolet absorption spectra of the three complexes showed maxima at wavelengths associated with two unsaturated systems containing four and six conjugated double bonds respectively. The two unsaturated components were separated by countercurrent distribution and shown to correspond to the two active fractions. The tetraene has been named endomycin A, and the hexaene endomycin B. Flavacid, a polyene complex also containing tetraene and hexaene components, is not identical with endomycin.

ESTRADIOL-17β IN THE EGGS OF THE AMERICAN LOBSTER, HOMARUS AMERICANUS

1961 ◽  
Vol 39 (4) ◽  
pp. 659-662 ◽  
Author(s):  
Robert D. Lisk
Keyword(s):  
Paper Chromatography ◽  
Estrogenic Activity ◽  
Active Material ◽  
Homarus Americanus ◽  
Countercurrent Distribution ◽  
Biologically Active ◽  
American Lobster ◽  
Materials Analysis ◽  
Estradiol 17Β ◽  
Bio Assay

Eggs of the American lobster, Homarus americanus, were examined for estrogenic materials. Analysis by the Astwood 6-hour assay indicated 0.26 to 0.32 μg of estrogenic activity per kilogram of eggs. Analysis by countercurrent distribution, paper chromatography, and bio-assay strongly suggests that the biologically active material is estradiol-17β.

99mTechnetium-Unithiol Complex, a New Pharmaceutical for Kidney Scintigraphy

1976 ◽  
Vol 15 (06) ◽  
pp. 282-286 ◽  
Author(s):  
M. Rembelska ◽  
M. Ogiński
Keyword(s):  
Paper Chromatography ◽  
Rat Kidneys

SummaryThe investigations were carried out with a newly syn-thesised, original 99mTc-Unithiol complex (Unithiol-2,3-dimercaptopropansulphonate). It accumulates in the kidneys and enables their scintigraphic examination at 90—180 minutes after the administration. The study was performed on rats, rabbits and dogs. 99mTc-Unithiol complex accumulation in rat kidneys was 34,9%, 44,5% and 67,8% of dose after 1, 2 and 3 h respectively.Good reability of the scintigraphic picture of the kidneys in rabbits and dogs confirms the accumulative capability of the complex in these organs. It was found by means of ascending paper chromatography that the purity of the 99mTc-Unithiol complex exceeded 90%.

PAPER CHROMATOGRAPHY OF URINARY 17-KETOSTEROIDS IN CHILDREN

1964 ◽  
Vol 45 (3_Suppl) ◽  
pp. S33 ◽  
Author(s):  
J. Bertrand ◽  
S. Roux ◽  
C. Cotte
Keyword(s):  
Paper Chromatography

A SIMPLE METHOD FOR THE DETECTION OF URINARY UNCONJUGATED CORTISOL BY PAPER CHROMATOGRAPHY

1964 ◽  
Vol 47 (3) ◽  
pp. 466-468 ◽  
Author(s):  
S. B. Pal
Keyword(s):  
Paper Chromatography ◽  
Human Urine ◽  
Specific Method ◽  
Simple Method ◽  
Normal Human

ABSTRACT Unconjugated corticosteroids are extracted from normal human urine and the urine of patients with rheumatic disorders treated with synthetic corticosteroids and corticotrophin. A simple and specific method using paper chromatography has been developed to detect the unconjugated cortisol in urine.

VANILLINSÄURE ALS ENDPRODUKT DES STOFFWECHSELS VON ADRENALIN UND NORADRENALIN. II.

1963 ◽  
Vol 44 (1) ◽  
pp. 101-106 ◽  
Author(s):  
Wilhelm Dirscherl ◽  
Helmut Thomas
Keyword(s):  
Rat Liver ◽  
Paper Chromatography ◽  
Column Chromatography ◽  
Vanillic Acid ◽  
Hippuric Acid ◽  
Single Spot ◽  
Solvent Systems ◽  
Perfusion Experiment ◽  
Kinetics Of

ABSTRACT Perfusion of rat liver with vanillic acid yielded only one metabolite. In paper chromatography with three different solvent systems, the substance showed the same RF-values as vanillyolglycine (3-methoxy-4-hydroxyhippuric acid) and in mixed chromatograms there was only one single spot. After separation by column chromatography, the UV- and IRspectra of the reaction product were identical with those of 3-methoxy4-hydroxy-hippuric acid. During the perfusion experiment, the kinetics of the conjugation were investigated.

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