Integrated Microfluidic Capillary Electrophoresis-Electrospray Ionization Devices with Online MS Detection for the Separation and Characterization of Intact Monoclonal Antibody Variants

Erin A. Redman; Nicholas G. Batz; J. Scott Mellors; J. Michael Ramsey

doi:10.1021/ac503964j

Integrated Microfluidic Capillary Electrophoresis-Electrospray Ionization Devices with Online MS Detection for the Separation and Characterization of Intact Monoclonal Antibody Variants

Analytical Chemistry ◽

10.1021/ac503964j ◽

2015 ◽

Vol 87 (4) ◽

pp. 2264-2272 ◽

Cited By ~ 72

Author(s):

Erin A. Redman ◽

Nicholas G. Batz ◽

J. Scott Mellors ◽

J. Michael Ramsey

Keyword(s):

Monoclonal Antibody ◽

Capillary Electrophoresis ◽

Electrospray Ionization ◽

Ms Detection

Download Full-text

Characterization of New Cyclic d,l-α-Alternate Amino Acid Peptides by Capillary Electrophoresis Coupled to Electrospray Ionization Mass Spectrometry

Methods in Molecular Biology - Electrophoretic Separation of Proteins ◽

10.1007/978-1-4939-8793-1_27 ◽

2018 ◽

pp. 315-326

Author(s):

María Dámaris Cortez-Díaz ◽

Fanny d’Orlyé ◽

Anne Varenne

Keyword(s):

Mass Spectrometry ◽

Capillary Electrophoresis ◽

Amino Acid ◽

Electrospray Ionization ◽

Electrospray Ionization Mass Spectrometry ◽

Ionization Mass Spectrometry ◽

Ionization Mass

Download Full-text

Isolation and characterization of β-cyclodextrin sulfates by preparative gradient polyacrylamide gel electrophoresis, capillary electrophoresis and electrospray ionization—mass spectrometry

Electrophoresis ◽

10.1002/elps.1150191517 ◽

1998 ◽

Vol 19 (15) ◽

pp. 2677-2681 ◽

Cited By ~ 4

Author(s):

Ronald E. Hileman ◽

Marshall M. Siegel ◽

Keiko Tabei ◽

Kuberan Balagurunathan ◽

Robert J. Linhardt

Keyword(s):

Mass Spectrometry ◽

Capillary Electrophoresis ◽

Electrospray Ionization ◽

Electrospray Ionization Mass Spectrometry ◽

Gel Electrophoresis ◽

Polyacrylamide Gel Electrophoresis ◽

Polyacrylamide Gel ◽

Ionization Mass ◽

Isolation And Characterization

Download Full-text

Frontal Analysis Capillary Electrophoresis Hyphenated to Electrospray Ionization Mass Spectrometry for the Characterization of the Antithrombin/Heparin Pentasaccharide Complex

Analytical Chemistry ◽

10.1021/ac070146h ◽

2007 ◽

Vol 79 (13) ◽

pp. 4987-4993 ◽

Cited By ~ 41

Author(s):

Soraya Fermas ◽

Florence Gonnet ◽

Anne Varenne ◽

Pierre Gareil ◽

Régis Daniel

Keyword(s):

Mass Spectrometry ◽

Capillary Electrophoresis ◽

Electrospray Ionization ◽

Electrospray Ionization Mass Spectrometry ◽

Ionization Mass Spectrometry ◽

Ionization Mass ◽

Frontal Analysis

Download Full-text

Comparing and Combining Capillary Electrophoresis Electrospray Ionization Mass Spectrometry and Nano–Liquid Chromatography Electrospray Ionization Mass Spectrometry for the Characterization of Post-translationally Modified Histones

Molecular & Cellular Proteomics ◽

10.1074/mcp.m112.024109 ◽

2013 ◽

Vol 12 (9) ◽

pp. 2640-2656 ◽

Cited By ~ 56

Author(s):

Bettina Sarg ◽

Klaus Faserl ◽

Leopold Kremser ◽

Bernhard Halfinger ◽

Roberto Sebastiano ◽

...

Keyword(s):

Mass Spectrometry ◽

Capillary Electrophoresis ◽

Liquid Chromatography ◽

Electrospray Ionization ◽

Electrospray Ionization Mass Spectrometry ◽

Ionization Mass Spectrometry ◽

Ionization Mass ◽

Nano Liquid Chromatography

Download Full-text

Immuno-capillary electrophoresis for the characterization of a monoclonal antibody against DNA

Journal of Chromatography A ◽

10.1016/0021-9673(96)00425-6 ◽

1996 ◽

Vol 744 (1-2) ◽

pp. 285-294 ◽

Cited By ~ 36

Author(s):

Niels H.H. Heegaard ◽

Dorthe T. Olsen ◽

Karen-Louise P. Larsen

Keyword(s):

Monoclonal Antibody ◽

Capillary Electrophoresis

Download Full-text

Capillary electrophoresis– electrospray ionization– mass spectrometry for the characterization of natural organic matter: An evaluation with free flow electrophoresis-off-line flow injection electrospray ionization-mass spectrometry

Electrophoresis ◽

10.1002/elps.200305566 ◽

2003 ◽

Vol 24 (17) ◽

pp. 3057-3066 ◽

Cited By ~ 16

Author(s):

Philippe Schmitt-Kopplin ◽

Antonius Kettrup

Keyword(s):

Mass Spectrometry ◽

Organic Matter ◽

Capillary Electrophoresis ◽

Electrospray Ionization ◽

Natural Organic Matter ◽

Electrospray Ionization Mass Spectrometry ◽

Ionization Mass Spectrometry ◽

Ionization Mass ◽

Line Flow

Download Full-text

Characterization of a synthetic 37-residue fragment of a monoclonal antibody against herpes virus by capillary electrophoresis/electrospray (ionspray) mass spectrometry and252Cf plasma desorption mass spectrometry

Biological Mass Spectrometry ◽

10.1002/bms.1200230608 ◽

1994 ◽

Vol 23 (6) ◽

pp. 346-352 ◽

Cited By ~ 7

Author(s):

R. Kostiainen ◽

E. Lasonder ◽

W. Bloemhoff ◽

P. A. van Veelen ◽

G. W. Welling ◽

...

Keyword(s):

Mass Spectrometry ◽

Monoclonal Antibody ◽

Capillary Electrophoresis ◽

Herpes Virus ◽

Desorption Mass Spectrometry ◽

Plasma Desorption Mass Spectrometry ◽

Plasma Desorption ◽

Herpès Virus

Download Full-text

Characterization of the F(ab′)2 fragment of a murine monoclonal antibody using capillary isoelectric focusing and electrospray ionization mass spectrometry

Journal of Chromatography A ◽

10.1016/s0021-9673(98)00344-6 ◽

1998 ◽

Vol 816 (1) ◽

pp. 49-58 ◽

Cited By ~ 26

Author(s):

Marie-Luise Hagmann ◽

Christine Kionka ◽

Martin Schreiner ◽

Christine Schwer

Keyword(s):

Mass Spectrometry ◽

Monoclonal Antibody ◽

Electrospray Ionization ◽

Isoelectric Focusing ◽

Electrospray Ionization Mass Spectrometry ◽

Murine Monoclonal Antibody ◽

Ionization Mass Spectrometry ◽

Ionization Mass ◽

Capillary Isoelectric Focusing

Download Full-text

Characterization of Single-Isomer, Heptasulfated β-Cyclodextrins by Electrospray Ionization Mass Spectrometry and Indirect UV Detection Capillary Electrophoresis

Analytical Chemistry ◽

10.1021/ac971103b ◽

1998 ◽

Vol 70 (14) ◽

pp. 3042-3045 ◽

Cited By ~ 14

Author(s):

Pavel V. Bondarenko ◽

Barbara Wolf ◽

Hong Cai ◽

J. Bryan Vincent ◽

Ronald D. Macfarlane ◽

...

Keyword(s):

Mass Spectrometry ◽

Capillary Electrophoresis ◽

Electrospray Ionization ◽

Electrospray Ionization Mass Spectrometry ◽

Uv Detection ◽

Ionization Mass Spectrometry ◽

Ionization Mass ◽

Indirect Uv Detection ◽

Single Isomer

Download Full-text

Isolation and characterization of protoporphyrin glycoconjugates from rat Harderian gland by HPLC, capillary electrophoresis and HPLC/electrospray ionization MS

Biochemical Journal ◽

10.1042/bj3470757 ◽

2000 ◽

Vol 347 (3) ◽

pp. 757-761 ◽

Cited By ~ 17

Author(s):

Chang Kee LIM ◽

M. Aaqib RAZZAQUE ◽

Jinli LUO ◽

Peter B. FARMER

Keyword(s):

Capillary Electrophoresis ◽

Electrospray Ionization ◽

Harderian Gland ◽

Tandem Ms ◽

Isolation And Characterization ◽

Harderian Glands ◽

On Line ◽

Identification And Characterization

It has been widely reported that the Harderian gland, present in most vertebrates, accumulates high levels of porphyrins, particularly protoporphyrin. The present study describes the extraction, identification and characterization of a group of hitherto unreported protoporphyrin glycoconjugates in the rat Harderian gland using HPLC, capillary electrophoresis, on-line HPLC/electrospray ionization MS and tandem MS. The major glycoconjugate was identified as protoporphyrin-1-O-acyl β-xyloside with a smaller amount of protoporphyrin-1-O-acyl β-glucoside also detected. In the Harderian glands studied, 50-70% of the porphyrins present were in the form of protoporphyrin glycoconjugates. This is the first reported occurrence of glycoconjugates of porphyrins in Nature and suggests that previous studies have wrongly identified the major porphyrin in the Harderian gland as the unconjugated protoporphyrin.

Download Full-text