Insights into the Conformations of Three Structurally Diverse Proteins: Cytochrome c, p53, and MDM2, Provided by Variable-Temperature Ion Mobility Mass Spectrometry

2015 ◽  
Vol 87 (6) ◽  
pp. 3231-3238 ◽  
Author(s):  
Eleanor R. Dickinson ◽  
Ewa Jurneczko ◽  
Kamila J. Pacholarz ◽  
David J. Clarke ◽  
Matthew Reeves ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Cytochrome C ◽  
Ion Mobility ◽  
Variable Temperature ◽  
Ion Mobility Mass Spectrometry

Molecular Insights into the Thermal Stability of mAbs with Variable-Temperature Ion-Mobility Mass Spectrometry

ChemBioChem ◽  
2015 ◽  
Vol 17 (1) ◽  
pp. 46-51 ◽  
Author(s):  
Kamila J. Pacholarz ◽  
Shirley J. Peters ◽  
Rachel A. Garlish ◽  
Alistair J. Henry ◽  
Richard J. Taylor ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Thermal Stability ◽  
Ion Mobility ◽  
Variable Temperature ◽  
Ion Mobility Mass Spectrometry ◽  
Thermal Stability Of

scholarly journals Gas-phase protein conformation/multimer ion formation by electrospray ion mobility-mass spectrometry: bovine insulin and ubiquitin

2016 ◽  
Vol 374 (2079) ◽  
pp. 20150368
Author(s):  
Kent J. Gillig
Keyword(s):  
Mass Spectrometry ◽  
Cytochrome C ◽  
Ion Mobility ◽  
Protein Concentration ◽  
Bovine Insulin ◽  
Ion Mobility Mass Spectrometry ◽  
Quantitative Mass Spectrometry ◽  
Oligomer Formation ◽  
Bovine Ubiquitin ◽  
Disulfide Linkages

Ion mobility-mass spectrometry (IMMS) is a very attractive method for studies in structural biology because of the ability of rapid isolation by nearly simultaneous m/z characterization and size separation, leading to an emergence of IMMS as a complimentary biochemical tool. Earlier, we developed a method based on varying the protein concentration in solution prior to electrospray ionization (ESI) with subsequent m/z selection and dissociation of protein multimers by IMMS of cytochrome c. The focus of this work will be to correctly distinguish truly different ion conformations formed by ESI versus homomultimeric complexes with the same m/z for well-studied proteins bovine ubiquitin and insulin. These proteins were chosen due to their large difference in solution phase structures: insulin tightly bound by disulfide linkages, and ubiquitin—a protein that may adopt a range of states from compact to extended. Our preliminary results, as with cytochrome c reveal false negatives for protein oligomer formation and false positives for protein conformational states. In addition, these results will be couched in terms of the need for quantification of IMMS analysis of proteins given the total area under IMMS peaks can also distinguish conformation versus aggregation as higher order oligomers have more mass per ion. This article is part of the themed issue ‘Quantitative mass spectrometry’.

Rapid Diagnosis of Parkinson’s Disease from Sebum using Paper Spray Ionisation Ion Mobility Mass Spectrometry

2020 ◽  
Author(s):  
Depanjan Sarkar ◽  
Drupad Trivedi ◽  
Eleanor Sinclair ◽  
Sze Hway Lim ◽  
Caitlin Walton-Doyle ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Ion Mobility ◽  
Neurodegenerative Disorder ◽  
Treatment Options ◽  
Ion Mobility Mass Spectrometry ◽  
Paper Spray ◽  
Molecular Features ◽  
Validation Set

Parkinson’s disease (PD) is the second most common neurodegenerative disorder for which identification of robust biomarkers to complement clinical PD diagnosis would accelerate treatment options and help to stratify disease progression. Here we demonstrate the use of paper spray ionisation coupled with ion mobility mass spectrometry (PSI IM-MS) to determine diagnostic molecular features of PD in sebum. PSI IM-MS was performed directly from skin swabs, collected from 34 people with PD and 30 matched control subjects as a training set and a further 91 samples from 5 different collection sites as a validation set. PSI IM-MS elucidates ~ 4200 features from each individual and we report two classes of lipids (namely phosphatidylcholine and cardiolipin) that differ significantly in the sebum of people with PD. Putative metabolite annotations are obtained using tandem mass spectrometry experiments combined with accurate mass measurements. Sample preparation and PSI IM-MS analysis and diagnosis can be performed ~5 minutes per sample offering a new route to for rapid and inexpensive confirmatory diagnosis of this disease.

P‐97: Separation of Isomeric OLED Materials Using Ion Mobility‐Mass Spectrometry (IM‐MS)

2021 ◽  
Vol 52 (1) ◽  
pp. 1444-1447
Author(s):  
Hirotaka Shioji ◽  
Azusa Uematsu ◽  
Motoshi Onoda ◽  
Keiko Matsuda ◽  
Keisuke Sawada ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Ion Mobility ◽  
Ion Mobility Mass Spectrometry
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