Identification of Unfolding and Dissociation Pathways of Superoxide Dismutase in the Gas Phase by Ion-Mobility Separation and Tandem Mass Spectrometry

2014 ◽  
Vol 86 (23) ◽  
pp. 11599-11605 ◽  
Author(s):  
Xiaoyu Zhuang ◽  
Shu Liu ◽  
Ruixing Zhang ◽  
Fengrui Song ◽  
Zhiqiang Liu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Superoxide Dismutase ◽  
Tandem Mass Spectrometry ◽  
Gas Phase ◽  
Ion Mobility ◽  
Tandem Mass ◽  
Mobility Separation ◽  
Ion Mobility Separation

Combining tandem mass spectrometry with ion mobility separation to determine the architecture of polydisperse proteins

2015 ◽  
Vol 377 ◽  
pp. 663-671 ◽  
Author(s):  
Dale A. Shepherd ◽  
Michael T. Marty ◽  
Kevin Giles ◽  
Andrew J. Baldwin ◽  
Justin L.P. Benesch
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Ion Mobility ◽  
Tandem Mass ◽  
Mobility Separation ◽  
Ion Mobility Separation

scholarly journals Ion mobility separation of deprotonated oligosaccharide isomers – evidence for gas-phase charge migration

2016 ◽  
Vol 52 (83) ◽  
pp. 12353-12356 ◽  
Author(s):  
W. B. Struwe ◽  
C. Baldauf ◽  
J. Hofmann ◽  
P. M. Rudd ◽  
K. Pagel
Keyword(s):  
Mass Spectrometry ◽  
Gas Phase ◽  
Quantum Chemical Calculations ◽  
Ion Mobility ◽  
Quantum Chemical ◽  
Ion Mobility Mass Spectrometry ◽  
Charge Migration ◽  
Mobility Separation ◽  
Ion Mobility Separation

Ion mobility-mass spectrometry in combination with quantum chemical calculations provides evidence that the charge in deprotonated glycan ions is mobile.

Spatial Metabolomics of the Human Kidney Using MALDI Trapped Ion Mobility Imaging Mass Spectrometry

2020 ◽  
Author(s):  
Elizabeth Neumann ◽  
Lukasz Migas ◽  
Jamie L. Allen ◽  
Richard Caprioli ◽  
Raf Van de Plas ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Ion Mobility ◽  
Imaging Mass Spectrometry ◽  
Structural Complexity ◽  
Human Kidney ◽  
Resolving Power ◽  
Mobility Separation ◽  
Trapped Ion ◽  
Mobility Data ◽  
Ion Mobility Separation

<div> <div> <p>Small metabolites are essential for normal and diseased biological function but are difficult to study because of their inherent structural complexity. MALDI imaging mass spectrometry (IMS) of small metabolites is particularly challenging as MALDI matrix clusters are often isobaric with metabolite ions, requiring high resolving power instrumentation or derivatization to circumvent this issue. An alternative to this is to perform ion mobility separation before ion detection, enabling the visualization of metabolites without the interference of matrix ions. Here, we use MALDI timsTOF IMS to image small metabolites at high spatial resolution within the human kidney. Through this, we have found metabolites, such as arginic acid, acetylcarnitine, and choline that localize to the cortex, medulla, and renal pelvis, respectively. We have also demonstrated that trapped ion mobility spectrometry (TIMS) can resolve matrix peaks from metabolite signal and separate both isobaric and isomeric metabolites with different localizations within the kidney. The added ion mobility data dimension dramatically increased the peak capacity for molecular imaging experiments. Future work will involve further exploring the small metabolite profiles of human kidneys as a function of age, gender, and ethnicity.</p></div></div>

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