Profiling of Stable Isotope Enrichment in Specialized Metabolites Using Liquid Chromatography and Multiplexed Nonselective Collision-Induced Dissociation

2014 ◽  
Vol 86 (21) ◽  
pp. 10600-10607 ◽  
Author(s):  
Zhenzhen Wang ◽  
A. Daniel Jones
Keyword(s):  
Liquid Chromatography ◽  
Stable Isotope ◽  
Collision Induced Dissociation ◽  
Isotope Enrichment ◽  
Specialized Metabolites ◽  
Stable Isotope Enrichment

scholarly journals Stable isotope analysis spills the beans about spatial variance in trophic structure in a fish host – parasite system from the Vaal River System, South Africa

2019 ◽  
Author(s):  
Beric M. Gilbert ◽  
Milen Nachev ◽  
Maik A. Jochmann ◽  
Torsten C. Schmidt ◽  
Daniel Köster ◽  
...  
Keyword(s):  
South Africa ◽  
Stable Isotopes ◽  
Stable Isotope ◽  
Feeding Strategy ◽  
Isotope Analysis ◽  
Isotope Enrichment ◽  
Host Fish ◽  
Vaal River ◽  
Spatial Differences ◽  
Stable Isotope Enrichment

Abstract BackgroundStable isotope analysis offers a unique tool for comparing trophic interactions and food web architecture in ecosystems. This approach is based on analysis of the stable isotope ratios of carbon (13 C/ 12 C) and nitrogen (15 N/ 14 N) in organisms. Studies comparing stable isotope enrichment in hosts and parasites have shown that parasites are variably enriched in stable isotopes relative to the host.MethodsSharptooth catfish (Clarias gariepinus) were collected from six sites along the Vaal River, South Africa and were assessed for ectoparasites and endoparasites. Lamproglena clariae (Copepoda), Tetracampus ciliotheca and Proteocephalus glanduligerus (Cestoda), and larval Contracaecum sp. (Nematoda) were collected from the gills, intestine and mesenteries, respectively. Signatures of δ 13 C and δ 15 N were analysed in host muscle tissue and parasites using bulk stable isotope analysis.ResultsStable isotope enrichment was variable between parasites and the host fish, with L. clariae and the host sharing similar δ 15 N signatures and the endoparasites being depleted in both δ 13 C and δ 15 N relative to the host. Spatial differences in enrichment of stable isotopes were also identified. Fish and parasites collected from below the Vaal River Barrage were more enriched in the 15 N isotope than hosts and parasites collected from other sites. The opposite was identified for 13 C isotope fractionation.ConclusionDifferences in stable isotope enrichment in parasites infecting C. gariepinus could be related to the feeding strategy of each parasite species collected. Enrichment of δ 15 N in L. clariae would relate to the micropredatory nature of this parasite, which feeds on whole blood of the host fish. Depleted isotope levels in endoparasites could relate to their absorptive feeding strategy on metabolic by-products of the host. Spatial differences in both host and parasite tissues identified likely resulted from differences in the diet of the host and related with availability of prey items for the host fish.

scholarly journals Tracking Stable Isotope Enrichment in Tree Seedlings with Solid-State NMR Spectroscopy

2012 ◽  
Vol 2 (1) ◽  
Author(s):  
Charlotte E. Norris ◽  
Sylvie A. Quideau ◽  
Simon M. Landhäusser ◽  
Guy M. Bernard ◽  
Roderick E. Wasylishen
Keyword(s):  
Solid State ◽  
Nmr Spectroscopy ◽  
Stable Isotope ◽  
Solid State Nmr ◽  
Tree Seedlings ◽  
Isotope Enrichment ◽  
Solid State Nmr Spectroscopy ◽  
Stable Isotope Enrichment
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