Paper-Based Colorimetric Array Test Strip for Selective and Semiquantitative Multi-Ion Analysis: Simultaneous Detection of Hg2+, Ag+, and Cu2+

2014 ◽  
Vol 86 (17) ◽  
pp. 8829-8834 ◽  
Author(s):  
Li Liu ◽  
Hengwei Lin
Keyword(s):  
Simultaneous Detection ◽  
Test Strip ◽  
Ion Analysis

Simultaneous detection of phenacetin and its metabolite using a gold nanoparticle-based immunochromatographic test strip

The Analyst ◽  
2021 ◽  
Author(s):  
Jingjing Yao ◽  
Xin-Xin Xu ◽  
Liqiang Liu ◽  
Hua Kuang ◽  
Zhengyou Wang ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Gold Nanoparticle ◽  
Simultaneous Detection ◽  
Test Strip ◽  
Immunochromatographic Test ◽  
Immunochromatographic Strip

We have developed a sensitive and rapid gold nanoparticle-based immunochromatographic strip (GNP-ICS) for the detection of phenacetin (PNCT) using an anti-PNCT monoclonal antibody (mAb). The specific anti- PNCT mAb (2D6)...

scholarly journals Simultaneous Rapid Detection of Aflatoxin B1 and Ochratoxin A in Spices Using Lateral Flow Immuno-Chromatographic Assay

Foods ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2738
Author(s):  
Xue Zhao ◽  
Xindi Jin ◽  
Zhang Lin ◽  
Qi Guo ◽  
Bin Liu ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Aflatoxin B1 ◽  
High Performance ◽  
Simultaneous Detection ◽  
High Sensitivity ◽  
Strong Stability ◽  
Test Strip ◽  
Chromatography Analysis ◽  
Relative Standard ◽  
Strip Test

Spices are susceptible to contamination by aflatoxin B1 (AFB1) and ochratoxin A (OTA), which are both mycotoxins with high toxicity and carcinogenicity. In this study, we aimed to develop an immuno-chromatographic strip test for the simultaneous quantification of AFB1 and OTA in spices by spraying the coupled antigens AFB1–ovalbumin (AFB1–OVA) and OTA–ovalbumin (OTA–OVA) on a nitrocellulose membrane. The test strip had high sensitivity, good specificity, and strong stability. The detection limits of these two mycotoxins in Chinese prickly ash, pepper, chili, cinnamon, and aniseed were 5 μg/kg. The false positivity rate was 2%, and the false negativity rate was 0%. The maximum coefficient of variation was 4.28% between batches and 5.72% within batches. The average recovery rates of AFB1 and OTA in spices were 81.2–113.7% and 82.2–118.6%, respectively, and the relative standard deviation (RSD) was <10%. The actual sample detection was consistent with high performance liquid chromatography analysis results. Therefore, the immuno-chromatographic test strips developed in this study can be used for the on-site simultaneous detection of AFB1 and OTA in spices. This method would allow the relevant regulatory agencies to strengthen supervision in an effort to reduce the possible human health hazards of such contaminated spices.

scholarly journals Multiplex Recombinase Polymerase Amplification Assay for the Simultaneous Detection of Three Foodborne Pathogens in Seafood

Foods ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 278 ◽  
Author(s):  
Biao Ma ◽  
Jiali Li ◽  
Kai Chen ◽  
Xiaoping Yu ◽  
Chuanxin Sun ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Foodborne Pathogens ◽  
Vibrio Parahaemolyticus ◽  
Salmonella Enteritidis ◽  
Simultaneous Detection ◽  
Test Strip ◽  
Food Samples ◽  
Recombinase Polymerase Amplification ◽  
Quantitative Detection ◽  
Test Strip Reader

Foodborne pathogens can cause foodborne illness. In reality, one food sample may carry more than one pathogen. A rapid, sensitive, and multiple target method for bacteria detection is crucial in food safety. For the simultaneous detection of Staphylococcus aureus, Vibrio parahaemolyticus, and Salmonella Enteritidis, multi-objective recombinase polymerase amplification (RPA) combined with a lateral flow dipstick (LFD) was developed in this study. The whole process, including amplification and reading, can be completed in 15 min at 37 °C. The detection limits were 2.6 × 101 CFU/mL for Staphylococcus aureus, 7.6 × 101 CFU/mL for Vibrio parahaemolyticus, and 1.29 × 101 CFU/mL for Salmonella Enteritidis. Moreover, colored signal intensities on test lines were measured by a test strip reader to achieve quantitative detection for Staphylococcus aureus (R2 = 0.9903), Vibrio parahaemolyticus (R2 = 0.9928), and Salmonella Enteritidis (R2 = 0.9945). In addition, the method demonstrated good recoveries (92.00%–107.95%) in the testing of spiked food samples. Therefore, the multiplex LFD-RPA assay is a feasible method for the rapid, sensitive, and quantitative detection of bacterial pathogens in seafood.

Rapid simultaneous detection of fumonisin B1 and deoxynivalenol in grain by immunochromatographic test strip

2020 ◽  
Vol 606 ◽  
pp. 113878
Author(s):  
Xi Huang ◽  
Xinyu Huang ◽  
Jianhua Xie ◽  
Xiujiang Li ◽  
Zhibing Huang
Keyword(s):  
Simultaneous Detection ◽  
Fumonisin B1 ◽  
Test Strip ◽  
Immunochromatographic Test

A dextran mediated multicolor immunochromatographic rapid test strip for visual and instrumental simultaneous detection of Vibrio cholera O1 (Ogawa) and Clostridium botulinum toxin A

2017 ◽  
Vol 184 (12) ◽  
pp. 4817-4825 ◽  
Author(s):  
Morteza Gharaat ◽  
Reza H. Sajedi ◽  
Maryam Shanehsaz ◽  
Nezam Jalilian ◽  
Manouchehr Mirshahi ◽  
...  
Keyword(s):  
Botulinum Toxin ◽  
Clostridium Botulinum ◽  
Botulinum Toxin A ◽  
Simultaneous Detection ◽  
Rapid Test ◽  
Test Strip ◽  
Toxin A ◽  
Vibrio Cholera
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