scholarly journals A Generalizable, Tunable Microfluidic Platform for Delivering Fast Temporally Varying Chemical Signals to Probe Single-Cell Response Dynamics

2014 ◽  
Vol 86 (20) ◽  
pp. 10138-10147 ◽  
Author(s):  
Loice Chingozha ◽  
Mei Zhan ◽  
Cheng Zhu ◽  
Hang Lu
Keyword(s):  
Single Cell ◽  
Cell Response ◽  
Chemical Signals ◽  
Response Dynamics ◽  
Microfluidic Platform ◽  
Single Cell Response

scholarly journals Two-level modeling approach to identify the regulatory dynamics capturing drug response heterogeneity in single-cells

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Madalena Chaves ◽  
Luis C. Gomes-Pereira ◽  
Jérémie Roux
Keyword(s):  
Drug Resistance ◽  
Single Cell ◽  
Cell Response ◽  
Drug Response ◽  
Single Cells ◽  
Reaction Model ◽  
Population Heterogeneity ◽  
Cellular Heterogeneity ◽  
Response Dynamics ◽  
Modeling Approach

AbstractSingle-cell multimodal technologies reveal the scales of cellular heterogeneity impairing cancer treatment, yet cell response dynamics remain largely underused to decipher the mechanisms of drug resistance they take part in. As the phenotypic heterogeneity of a clonal cell population informs on the capacity of each single-cell to recapitulate the whole range of observed behaviors, we developed a modeling approach utilizing single-cell response data to identify regulatory reactions driving population heterogeneity in drug response. Dynamic data of hundreds of HeLa cells treated with TNF-related apoptosis-inducing ligand (TRAIL) were used to characterize the fate-determining kinetic parameters of an apoptosis receptor reaction model. Selected reactions sets were augmented to incorporate a mechanism that leads to the separation of the opposing response phenotypes. Using a positive feedback loop motif to identify the reaction set, we show that caspase-8 is able to encapsulate high levels of heterogeneity by introducing a response delay and amplifying the initial differences arising from natural protein expression variability. Our approach enables the identification of fate-determining reactions that drive the population response heterogeneity, providing regulatory targets to curb the cell dynamics of drug resistance.

scholarly journals 2P325 Analyzing the single cell response of macrophage to the antigen stimulation (I) Development of antigen-stimulation and analysis system

2005 ◽  
Vol 45 (supplement) ◽  
pp. S201
Author(s):  
H. Shindou ◽  
K. Orita ◽  
K. Matsumura ◽  
Y. Wakamoto ◽  
H. Ishimoto ◽  
...  
Keyword(s):  
Single Cell ◽  
Cell Response ◽  
Antigen Stimulation ◽  
Analysis System ◽  
Single Cell Response

scholarly journals Single cell response landscape of graded Nodal signaling in zebrafish explants

2021 ◽  
Author(s):  
Tao Cheng ◽  
Yanyi Xing ◽  
Yunfei Li ◽  
Cong Liu ◽  
Ying Huang ◽  
...  
Keyword(s):  
Single Cell ◽  
Cell Fate ◽  
Cell Response ◽  
Expression Patterns ◽  
Cell Types ◽  
Nodal Signaling ◽  
Animal Pole ◽  
Fate Decision ◽  
Single Cell Response

Nodal, as a morphogen, plays important roles in cell fate decision, pattern formation and organizer function. But because of the complex context in vivo and technology limitations, systematic studying of genes, cell types and patterns induced by Nodal alone is still missing. Here, by using a relatively simplified model, the zebrafish blastula animal pole explant avoiding additional instructive signals and prepatterns, we constructed a single cell response landscape of graded Nodal signaling, identified 105 Nodal immediate targets and depicted their expression patterns. Our results show that Nodal signaling is sufficient to induce anterior-posterior patterned axial mesoderm and head structure. Surprisingly, the endoderm induced by Nodal alone is mainly the anterior endoderm which gives rise to the pharyngeal pouch only, but not internal organs. Among the 105 Nodal targets, we identified 14 genes carrying varying levels of axis induction capability. Overall, our work provides new insights for understanding of the Nodal function and a valuable resource for future studies of patterning and morphogenesis induced by it.

scholarly journals Real-time single-cell response to stiffness

2010 ◽  
Vol 107 (38) ◽  
pp. 16518-16523 ◽  
Author(s):  
D. Mitrossilis ◽  
J. Fouchard ◽  
D. Pereira ◽  
F. Postic ◽  
A. Richert ◽  
...  
Keyword(s):  
Single Cell ◽  
Real Time ◽  
Cell Response ◽  
Single Cell Response

Single-Cell Response to the Rigidity of Semiconductor Nanomembranes on Compliant Substrates

2020 ◽  
Vol 12 (9) ◽  
pp. 10697-10705 ◽  
Author(s):  
Nadeem Abdul ◽  
Matthew N. Rush ◽  
Jiri Nohava ◽  
Ursula Amezcua ◽  
Andrew P. Shreve ◽  
...  
Keyword(s):  
Single Cell ◽  
Cell Response ◽  
Compliant Substrates ◽  
Single Cell Response

scholarly journals 2P326 Analyzing the single cell response of macrophage to the antigen stimulation (II) Processing of acquired information in the single cell

2005 ◽  
Vol 45 (supplement) ◽  
pp. S201
Author(s):  
K. Orita ◽  
H. Shindou ◽  
K. Matsumura ◽  
Y. Wakamoto ◽  
H. Ishimoto ◽  
...  
Keyword(s):  
Single Cell ◽  
Cell Response ◽  
Antigen Stimulation ◽  
Single Cell Response

scholarly journals Single Cell Response to Periodic Environmental Stimuli using a Microfluidic Bioreactor

2014 ◽  
Vol 106 (2) ◽  
pp. 225a
Author(s):  
Eric M. Johnson Chavarria ◽  
Utsav Agrawal ◽  
Melikhan Tanyeri ◽  
Thomas E. Kuhlman ◽  
Charles M. Schroeder
Keyword(s):  
Single Cell ◽  
Cell Response ◽  
Environmental Stimuli ◽  
Single Cell Response
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