Rapid Isoelectric Point Determination in a Miniaturized Preparative Separation Using Jet-Dispensed Optical pH Sensors and Micro Free-Flow Electrophoresis

2014 ◽  
Vol 86 (19) ◽  
pp. 9533-9539 ◽  
Author(s):  
Christin Herzog ◽  
Erik Beckert ◽  
Stefan Nagl
Keyword(s):  
Isoelectric Point ◽  
Free Flow ◽  
Preparative Separation ◽  
Ph Sensors ◽  
Optical Ph Sensors ◽  
Point Determination ◽  
Micro Free Flow Electrophoresis

scholarly journals Continuous on-chip fluorescence labelling, free-flow isoelectric focusing and marker-free isoelectric point determination of proteins and peptides

Lab on a Chip ◽  
2016 ◽  
Vol 16 (9) ◽  
pp. 1565-1572 ◽  
Author(s):  
Christin Herzog ◽  
Elisabeth Poehler ◽  
Andrea J. Peretzki ◽  
Sergey M. Borisov ◽  
Daniel Aigner ◽  
...  
Keyword(s):  
Real Time ◽  
Isoelectric Focusing ◽  
Isoelectric Point ◽  
Free Flow ◽  
Microfluidic Chips ◽  
Marker Free ◽  
Point Determination ◽  
On Chip ◽  
Proteins And Peptides

Presented are microfluidic chips that allow for continuous subsequent biomolecular labelling, free-flow isoelectric focusing and real-time isoelectric point monitoring.

scholarly journals Application of Optical pH Sensors in the Microfluidic Free-flow Isoelectric Focusing of Biomolecules

2015 ◽  
Vol 120 ◽  
pp. 175-179 ◽  
Author(s):  
Elisabeth Poehler ◽  
Christin Herzog ◽  
Simon A. Pfeiffer ◽  
Carsten Lotter ◽  
Andrea J. Peretzki ◽  
...  
Keyword(s):  
Isoelectric Focusing ◽  
Free Flow ◽  
Ph Sensors ◽  
Optical Ph Sensors

Electrokinetic Potential for Characterization of Nanosctructured Solid Flat Surfaces

2013 ◽  
Vol 25 ◽  
pp. 31-39 ◽  
Author(s):  
Zdeňka Kolská ◽  
Nikola Slepičková Kasálková ◽  
Jakub Siegel ◽  
Václav Švorčík
Keyword(s):  
Zeta Potential ◽  
Isoelectric Point ◽  
Electrokinetic Potential ◽  
Characteristic Parameter ◽  
Metal Nanostructures ◽  
Gold Deposition ◽  
Fast Analysis ◽  
Flat Surfaces ◽  
Acceptable Method ◽  
Point Determination

Electrokinetic potential (zeta potential) is a characteristic parameter for description of the surface chemistry of solid flat materials and it can be used for a fast analysis of materials modified by different chemical or physical methods. Due to its sensitivity, zeta potential is able to distinguish surface modified by coating with monolayers of various materials or nanostructures created after plasma treatment. Also metal nanostructures deposited on surfaces can be characterized by zeta potential. It can also be used for isoelectric point determination of materials. We present data on zeta potential in 0.001 mol/dm3 KCl at constant pH7.0 and also in pH range (2.5-7.0) for isoelectric point determination for pristine polymers PET, PTFE, PS, LDPE, HDPE, PLLA, PVF, PVDF, PMP and polyimides (Upilex R, Upilex S, Kapton). The zeta potential of selected polymers, modified by plasma and by chemical coatings (e.g. by biphenyldithiol or polyethyleneglycol) or by gold deposition was measured too. Zeta potentials of these modified materials were also studied to confirmation that electrokinetic analysis is acceptable method for their fast description.

Optimizing Band Width and Resolution in Micro-Free Flow Electrophoresis

2006 ◽  
Vol 78 (24) ◽  
pp. 8236-8244 ◽  
Author(s):  
Bryan R. Fonslow ◽  
Michael T. Bowser
Keyword(s):  
Free Flow ◽  
Band Width ◽  
Micro Free Flow Electrophoresis

scholarly journals Micro free-flow electrophoresis: theory and applications

2009 ◽  
Vol 394 (1) ◽  
pp. 187-198 ◽  
Author(s):  
Ryan T. Turgeon ◽  
Michael T. Bowser
Keyword(s):  
Free Flow ◽  
Micro Free Flow Electrophoresis

scholarly journals Improving sensitivity in micro-free flow electrophoresis using signal averaging

2009 ◽  
Vol 30 (8) ◽  
pp. 1342-1348 ◽  
Author(s):  
Ryan T. Turgeon ◽  
Michael T. Bowser
Keyword(s):  
Free Flow ◽  
Signal Averaging ◽  
Micro Free Flow Electrophoresis

Red light-excitable dual lifetime referenced optical pH sensors with intrinsic temperature compensation

The Analyst ◽  
2010 ◽  
Vol 135 (7) ◽  
pp. 1711 ◽  
Author(s):  
Sergey M. Borisov ◽  
Karl Gatterer ◽  
Ingo Klimant
Keyword(s):  
Temperature Compensation ◽  
Red Light ◽  
Ph Sensors ◽  
Optical Ph Sensors

Optical pH sensors using fluorescence decay time

1993 ◽  
Vol 11 (1-3) ◽  
pp. 421-424 ◽  
Author(s):  
Sonja Drazler ◽  
Max E. Lippitsch ◽  
Marc J.-P. Leiner
Keyword(s):  
Decay Time ◽  
Fluorescence Decay ◽  
Ph Sensors ◽  
Optical Ph Sensors ◽  
Fluorescence Decay Time

Moth wing scales as optical pH sensors

2012 ◽  
Vol 166-167 ◽  
pp. 824-828 ◽  
Author(s):  
Xining Zang ◽  
Yongwen Tan ◽  
Zhi Lv ◽  
Jiajun Gu ◽  
Di Zhang
Keyword(s):  
Ph Sensors ◽  
Optical Ph Sensors ◽  
Wing Scales
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