Quantitative analysis of volatile fatty acids in aqueous solution by gas chromatography

1967 ◽  
Vol 39 (13) ◽  
pp. 1652-1654 ◽  
Author(s):  
Vaidyanath. Mahadevan ◽  
Lauren. Stenroos
1987 ◽  
Vol 10 (9) ◽  
pp. 522-523 ◽  
Author(s):  
K. Kim ◽  
A. Zlatkis ◽  
E. C. Horning ◽  
B. S. Middleditch

1984 ◽  
Vol 30 (4) ◽  
pp. 542-546 ◽  
Author(s):  
M J Bennett ◽  
C E Bradey

Abstract We describe an isocratic cation-exchange chromatographic technique, with ultraviolet detection, for determination of urinary organic acids. The method is useful for screening urines from neonates and infants suspected of having an organic aciduria, prior to confirmatory analysis by gas chromatography-mass spectrometry. Advantages of this technique over conventional gas chromatography include ease of sample preparation and the simultaneous detection of volatile fatty acids; limitations include its lack of an identification system like that of mass spectrometry, the presence of nonultraviolet-absorbing fatty acids, and the relatively long retention times of phenolic compounds. Chromatograms for patients with methylmalonic aciduria, propionic aciduria, isovaleric aciduria, beta-ketothiolase deficiency, lactic aciduria, and glutaric aciduria type II are compared with those for a normal infant.


2022 ◽  
Vol 42 ◽  
pp. 01015
Author(s):  
E.V. Sheida ◽  
V.A. Ryazanov ◽  
K.S. Denisenko ◽  
O.V. Shoshina

The assessment of Asteráceae Family Plants (rhizomes and roots of elecampane and wormwood) influence on the process of methane formation in the rumen ecosystem and metabolic processes was carried out. Studies (in vitro) were carried out using ANKOM Daisy II incubator (modifications D200 and D200I) according to a specialized method. Rumen contents were obtained from beef bulls with chronic rumen fistula. Gas analysis of air and volatile fatty acids samples was performed by gas chromatography. The results of the study showed that different dosages of phytobiotic preparations did not significantly affect the characteristics of fermentation in vitro. Phytobiotic preparations of elecampane and wormwood reduce the production of methane in the ruminal fluid, which may be associated with various active components or dosages of their administration.


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