High Throughput Identification and Quantification of Anabolic Steroid Esters by Atmospheric Solids Analysis Probe Mass Spectrometry for Efficient Screening of Drug Preparations

2014 ◽  
Vol 86 (12) ◽  
pp. 5649-5655 ◽  
Author(s):  
Mickael Doué ◽  
Gaud Dervilly-Pinel ◽  
Audrey Gicquiau ◽  
Karinne Pouponneau ◽  
Fabrice Monteau ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Throughput ◽  
Anabolic Steroid ◽  
Solids Analysis ◽  
Efficient Screening ◽  
Identification And Quantification

scholarly journals Protein inference using PIA workflows and PSI standard file formats

2018 ◽  
Author(s):  
Julian Uszkoreit ◽  
Yasset Perez-Riverol ◽  
Britta Eggers ◽  
Katrin Marcus ◽  
Martin Eisenacher
Keyword(s):  
Mass Spectrometry ◽  
Open Source ◽  
High Throughput ◽  
Biological Samples ◽  
Shotgun Proteomics ◽  
Original Sample ◽  
Protein Inference ◽  
File Formats ◽  
Inference Algorithms ◽  
Identification And Quantification

AbstractProteomics using LC-MS/MS has become one of the main methods to analyze the proteins in biological samples in high-throughput. But the existing mass spectrometry instruments are still limited with respect to resolution and measurable mass ranges, which is one of the main reasons why shotgun proteomics is the major approach. Here, proteins are digested, which leads to the identification and quantification of peptides instead. While often neglected, the important step of protein inference needs to be conducted to infer from the identified peptides to the actual proteins in the original sample.In this work, we highlight some of the previously published and newly added features of the tool PIA – Protein Inference Algorithms, which helps the user with the protein inference of measured samples. We also highlight the importance of the usage of PSI standard file formats, as PIA is the only current software supporting all available standards used for spectrum identification and protein inference. Additionally, we briefly describe the benefits of working with workflow environments for proteomics analyses and show the new features of the PIA nodes for the KNIME Analytics Platform. Finally, we benchmark PIA against a recently published dataset for isoform detection.PIA is open source and available for download on GitHub (https://github.com/mpc-bioinformatics/pia) or directly via the community extensions inside the KNIME analytics platform.

154: Integration of TPSA and High-Throughput Mass Spectrometry Data Improves Prostate Cancer Prediction

2007 ◽  
Vol 177 (4S) ◽  
pp. 52-53
Author(s):  
Stefano Ongarello ◽  
Eberhard Steiner ◽  
Regina Achleitner ◽  
Isabel Feuerstein ◽  
Birgit Stenzel ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Mass Spectrometry ◽  
High Throughput ◽  
Mass Spectrometry Data ◽  
Cancer Prediction
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