Characterizing chemical systems with on-line computers and graphics: alkaline phosphatase catalyzed reaction

1979 ◽  
Vol 51 (11) ◽  
pp. 1747-1754 ◽  
Author(s):  
Jack W. Frazer ◽  
Lester P. Rigdon ◽  
Hal R. Brand ◽  
Charles L. Pomernacki ◽  
Thomas A. Brubaker
Keyword(s):  
Alkaline Phosphatase ◽  
Chemical Systems ◽  
On Line

Characterizing chemical systems with on-line computers and graphics

1979 ◽  
Vol 51 (11) ◽  
pp. 1739-1747 ◽  
Author(s):  
Jack W. Frazer ◽  
Lester P. Rigdon ◽  
Hal R. Brand ◽  
Charles L. Pomernacki
Keyword(s):  
Chemical Systems ◽  
On Line

scholarly journals Flow injection analysis of water. Part 1: Automatic preconcentration determination of sulphate, ammonia and iron(II)/iron(III)

1993 ◽  
Vol 15 (4) ◽  
pp. 141-146 ◽  
Author(s):  
J. S. Cosano ◽  
M. D. Luque de Castro ◽  
M. Valcárcel
Keyword(s):  
Flow Injection ◽  
Good Reproducibility ◽  
Chemical Systems ◽  
Sequential Determination ◽  
On Line ◽  
Exchange Material ◽  
Iron Complexation ◽  
Simple Flow ◽  
Ammonia Formation

This paper describes a simple flow-injection (FI) manifold for the determination of a variety of species in industrial water. The chemical systems involved in the determination of ammonia (formation of Indophenol Blue), sulfate (precipitation with Ba(II)), and iron (complexation with 1,10-phenanthroline with the help of a prior redox reaction for speciation) were selected so that a common manifold could be used for the sequential determination of batches of each analyte. A microcolumn of a suitable ion exchange material was used for on-line preconcentration of each analyte prior to injection; linear ranges for the determination of the analytes at the ng/ml levels were obtained with good reproducibility. The manifold and methods are ready for full automation.

On-line HPLC Determination of Enzymatic Activity of Alkaline Phosphatase in Natural Water Using Spectrofluorometric Detection

1993 ◽  
Vol 22 (2) ◽  
pp. 307-310 ◽  
Author(s):  
Akio Hirose ◽  
Yukihiro Esaka ◽  
Michiru Ohta ◽  
Hiroki Haraguchi
Keyword(s):  
Alkaline Phosphatase ◽  
Enzymatic Activity ◽  
Natural Water ◽  
Hplc Determination ◽  
On Line

Evaluation of Kinetic Enzyme Parameters by Use of a Small Computer Interfaced "Fast Analyzer"—An Addition to Automated Clinical Enzymology

1973 ◽  
Vol 19 (8) ◽  
pp. 908-918 ◽  
Author(s):  
T O Tiffany ◽  
D D Chilcote ◽  
C A Burtis
Keyword(s):  
Alkaline Phosphatase ◽  
Kinetic Parameters ◽  
Small Computer ◽  
Programming Approach ◽  
Initial Reaction ◽  
Placental Alkaline Phosphatase ◽  
Intestinal Alkaline Phosphatase ◽  
On Line ◽  
Clinical Enzymology ◽  
Glucose 6 Phosphate Dehydrogenase

Abstract The evaluation of steady-state enzyme kinetic parameters such as Km, Vmax, and Ki is useful in clinical enzymology, particularly where they can be used for the detection of enzyme variants and to assess the type of isoenzymes and the extent of their abnormal activity. The multiple-cuvet parallel-analysis feature of the Fast Analyzer permits one to evaluate one or more enzymic parameters in a single rotor. Both on-line measurement of initial reaction velocities and rapid off-line reduction of statistical data can be obtained by using the small-computer interfaced Fast Analyzer. Estimates of error in determining these parameters are also obtained, for use in comparing results in future analyses. We discuss the programming approach for adapting both simple and more complex statistical nonlinear regression data-fitting routines to the Fast Analyzer’s computer. We illustrate how the analyzer may be used to determine simple kinetic parameters for placental alkaline phosphatase and glucose-6-phosphate dehydrogenase and for the more complex "stripping out" of Km’s and Vmax’s for a heterogenous mixture of placental and intestinal alkaline phosphatase.

Alkaline phosphatase isoenzymes in serum determined by high-performance anion-exchange liquid chromatography with detection by enzyme reaction.

1988 ◽  
Vol 34 (12) ◽  
pp. 2406-2409 ◽  
Author(s):  
M T Parviainen ◽  
J H Galloway ◽  
J H Towers ◽  
J A Kanis
Keyword(s):  
Alkaline Phosphatase ◽  
Liquid Chromatography ◽  
Anion Exchange ◽  
High Performance ◽  
Enzyme Reaction ◽  
Anion Exchange Column ◽  
Alkaline Phosphatase Isoenzymes ◽  
On Line ◽  
Reproducible Method ◽  
L1 And L2

Abstract This rapid, reproducible method for separating and determining individual alkaline phosphatase (EC 3.1.3.1) isoenzymes in serum is based on high-performance liquid chromatography with a weak anion-exchange column (SynChropak AX 300). The isoenzymes so resolved are detected by using an on-line enzyme reaction followed by spectrophotometric monitoring at 405 nm of the 4-nitrophenol formed. Complete diagnostic profiles of the various isoenzymes present in normal and pathological sera are obtained within 20 min. The mean (and SD) normal concentrations of the bone B1 and intestinal isoenzymes in serum of adults were 3.7 (4.3) and 4.5 (3.9) U/L, respectively (n = 14), and of the bone isoenzyme B2 and liver isoenzymes L1 and L2, 5.8 (8.6), 33.0 (10.6), and 12.0 (4.8) U/L, respectively (n = 17). Concentrations of the B2 and L1 isoenzymes in adults over age 40 years differed significantly from those in adults younger than 40 years, that of bone isoenzyme being lower (P less than 0.05) and that of the liver isoenzyme being higher (P less than 0.001) in the younger adults.

Liver- and bone-derived isoenzymes of alkaline phosphatase in serum as determined by high-performance affinity chromatography

1990 ◽  
Vol 36 (2) ◽  
pp. 240-246 ◽  
Author(s):  
D J Anderson ◽  
E L Branum ◽  
J F O'Brien
Keyword(s):  
Alkaline Phosphatase ◽  
Affinity Chromatography ◽  
High Performance ◽  
Bone Alkaline Phosphatase ◽  
Phosphate Solution ◽  
Serum Samples ◽  
High Performance Affinity Chromatography ◽  
Nitrophenyl Phosphate ◽  
On Line ◽  
Two Peaks

Abstract To separate liver and bone alkaline phosphatase (ALP) isoenzymes in human serum, we used high-performance affinity chromatography (HPAC) on a column of wheat-germ lectin conjugated to 7-microns-diameter silica particles and an eluent containing N-acetyl-D-glucosamine (NAG). On-line spectrophotometric detection of ALP involved pumping diethanolamine-buffered p-nitrophenyl phosphate solution post-column. Bone and liver isoenzymes could be separated into two peaks with only 10% overlap when an exponential gradient was used. A linear-step gradient separated 80.9% of liver ALP and 91.6% of bone ALP in two distinct peaks. True bone and liver ALP peak areas for the linear-step gradient were determined by using correction factors, because each peak contained a co-eluted portion of the other ALP isoenzyme. The detection limit improved 10-fold over those of other techniques for ALP isoenzymes, owing to the relatively large sample that could be applied to the column. Correlation with a urea-inactivation procedure was reasonable for patients' serum samples (r = 0.98 and 0.79 for liver ALP and bone ALP, respectively).

scholarly journals Influence of Piping on On-Line Continuous Weighing of Materials inside Process Equipment: Theoretical Analysis and Experimental Verification

2021 ◽  
Vol 11 (11) ◽  
pp. 5246
Author(s):  
Yuanlin Jing ◽  
Feng Guo ◽  
Yiping Wang ◽  
Qunwu Huang
Keyword(s):  
Optimization Design ◽  
Operating Conditions ◽  
Beam Deflection ◽  
Process Equipment ◽  
Engineering Optimization ◽  
Continuous Contact ◽  
Chemical Systems ◽  
Theoretical Criterion ◽  
On Line ◽  
Continuous Heat

Due to the continuity and complexity of chemical systems, piping and operating conditions will have a significant effect on the on-line continuous weighing of materials inside process equipment. In this paper, a mathematical model of the weighing system considering piping and operating conditions was established based on the gas–liquid continuous heat transfer weighing process. A theoretical criterion which can be extended to any continuous weighing system of the materials inside equipment with connected piping is obtained through the mechanical derivation between the material mass, the cantilever beam deflection, the strain gage deformation, and the bridge output voltage. This criterion can effectively predict the influence of piping on weighing results with specific accuracy, and provide a basis for engineering optimization design. On this basis, a set of gas–liquid continuous contact weighing devices was built. The static/dynamic experimental results showed that the accuracy of the system meets the set requirements.

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