scholarly journals Digital Encoding of Cellular mRNAs Enabling Precise and Absolute Gene Expression Measurement by Single-Molecule Counting

2014 ◽  
Vol 86 (6) ◽  
pp. 2867-2870 ◽  
Author(s):  
Glenn K. Fu ◽  
Julie Wilhelmy ◽  
David Stern ◽  
H. Christina Fan ◽  
Stephen P. A. Fodor
Keyword(s):  
Gene Expression ◽  
Single Molecule ◽  
Gene Expression Measurement ◽  
Cellular Mrnas ◽  
Digital Encoding ◽  
Expression Measurement

Integrated on-capillary instrumentation for gene expression measurement directly from cells

2003 ◽  
Vol 24 (3) ◽  
pp. 458-465 ◽  
Author(s):  
Hiroko Matsunaga ◽  
Takashi Anazawa ◽  
Edward S. Yeung
Keyword(s):  
Gene Expression ◽  
Gene Expression Measurement ◽  
Expression Measurement

A Quantitative Free Convection DNA Amplifier

2007 ◽  
Author(s):  
John Daly ◽  
Mark Davies
Keyword(s):  
Gene Expression ◽  
Free Convection ◽  
Thermal Imaging ◽  
Medical Diagnostics ◽  
Gene Expression Measurement ◽  
Chain Reaction ◽  
Cycle Times ◽  
Imaging Results ◽  
Polymerase Chain ◽  
Expression Measurement

The Polymerase Chain Reaction (PCR) has been used extensively to amplify targeted nucleic acids for many applications in molecular biology and, increasingly, in medical diagnostics. Outlined in this paper is a PCR device which takes account of the advantages offered by free convection. The design is, in it fundamental format a time-wise isothermal well-based thermocycler. A temperature gradient induced across the well causes convection forces to circulate the sample through the required temperatures necessary for amplification. Quantitative amplification is demonstrated with real time measurements of SYBR Green I fluorescence within the free convective DNA amplifier. Amplification of an 86-bp fragment of the pGEM®-T vector (Promega) is performed in a 25μl volume in eight minutes. A 10-fold dilution series and methods for calculating effective cycle times are presented. Also detailed within this paper are PIV and thermal imaging results of the free convection cavity. This device presents an opportunity for the development of a practical and inexpensive gene-expression measurement system.

scholarly journals Normalization of gene expression measurement of tissue samples obtained by transurethral resection of bladder tumors

2016 ◽  
pp. 3369 ◽  
Author(s):  
Laura-Ancuta Pop ◽  
Valentina Pileczki ◽  
Roxana-Maria Cojocneanu-Petric ◽  
Bogdan Petrut ◽  
Cornelia Braicu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transurethral Resection ◽  
Gene Expression Measurement ◽  
Bladder Tumors ◽  
Tissue Samples ◽  
Expression Measurement
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