Prediction of retention times of the nucleosides and bases on reverse phase high performance liquid chromatography during gradient elution

1979 ◽  
Vol 51 (1) ◽  
pp. 34-38 ◽  
Author(s):  
Richard A. Hartwick ◽  
Charles M. Grill ◽  
Phyllis R. Brown
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Gradient Elution ◽  
Reverse Phase ◽  
Prediction Of Retention ◽  
Prediction Of Retention Times

A Simple and Rapid High-Performance Liquid Chromatography—Photodiode Array Method for Quantitation of Triacetin Contents From 30 Batches Produced in China

2020 ◽  
Vol 59 (1) ◽  
pp. 23-29
Author(s):  
Ran Li ◽  
Xi Pan ◽  
Hao Wang ◽  
Guoning Guo ◽  
Long Huang ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Energy Efficient ◽  
High Performance ◽  
Preparation Method ◽  
Gradient Elution ◽  
Sample Preparation Method ◽  
Formic Acid Solution ◽  
Reverse Phase ◽  
Photodiode Array

Abstract In our present study, the standard chemicals of triacetin were purified by reverse-phase and normal-phase semi-preparative high-performance liquid chromatography (HPLC), and 1H NMR and 13C NMR were employed to determine the purity and structure of triacetin. Moreover, a simple and rapid HPLC-photodiode array (PDA) method was developed to determine the contents of triacetin in 30 batches from different suppliers. The chromatographic separation was performed on a Phenomenex Gemini-NX C18 column (250 × 4.6 mm, 5 μm) using a gradient elution system of water and acetonitrile (contained 0.1% of formic acid) solution with a flow rate of 1.0 mL/min at 30°C at 210 nm. Sample preparation method is rapid and energy efficient, and the obtained sample have a good purity. Validation shows good specificity, linearity (R2 = 0.9995), precision, stability, repeatability (% RSD < 2.80) and the average recovery (99.72%) of triacetin. The content of triacetin in most samples is concentrated in 94–97%. This developed approach is simple, rapid, accurate and can be used to quickly determine the purity and the content of triacetin in plasticizers and filter plugs.

scholarly journals Novel Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) Method Development and Validation of Atorvastatin and Fenofibrate in Tablets

2020 ◽  
Vol 11 (02) ◽  
pp. 232-236
Author(s):  
Chandrasekhar Kudupudi ◽  
Manikandan Ayyar
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Related Compound ◽  
High Performance ◽  
Gradient Elution ◽  
Simultaneous Estimation ◽  
Reverse Phase ◽  
Compound A ◽  
Atorvastatin Calcium ◽  
Rp Hplc

A novel, simple, selective, precise, and accurate reverse-phase high-performance liquid chromatography (RP-HPLC) gradient method was developed for the simultaneous estimation of atorvastatin and fenofibrate in the combined formulation. The drugs atorvastatin calcium and fenofibrate were separated in the presence of their impurities atorvastatin related compound H, fenofibrate related compound A, and fenofibrate related compound B. The drugs and related compounds were separated on Kromasil C18 (250 x 4.6, 5μ) with reverse-phase gradient elution. Water adjusted pH 4.0 with phosphoric acid used as a buffer in pump A and acetonitrile used as a solvent in pump B as a mobile phase with gradient elution. The flow rate was 2.0 mL/min. 254 nm was the detection wavelength. The retention times were about 4.6 minutes for fenofibrate related compound A, 5.2 minutes for atorvastatin calcium, 5.7 minutes for fenofibrate related compound B, 8.7 minutes for atorvastatin related compound H, and 17.6 minutes for fenofibrate. The linearity ranges for atorvastatin calcium and fenofibrate were 5.00 to 15.00 and 80 to 240 mcg/mL, respectively, with correlation coefficient 0.999 for both. The proposed method validated statistically with respect to system suitability, specificity, linearity, precision, accuracy, range, robustness, and ruggedness. The method was accurate, linear, precise, specific, selective, and rapid suitable for the quantitative estimation of atorvastatin and fenofibrate in tablets.

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