Evaluation of a computer-controlled stopped-flow system for fundamental kinetic studies

1978 ◽  
Vol 50 (9) ◽  
pp. 1333-1337 ◽  
Author(s):  
Glen E. Mieling ◽  
Harry L. Pardue
Keyword(s):  
Flow System ◽  
Kinetic Studies ◽  
Stopped Flow ◽  
Computer Controlled

Study of the film formation process in liquid-liquid extraction flow injection analysis

1991 ◽  
Vol 56 (6) ◽  
pp. 1228-1237 ◽  
Author(s):  
Vlastimil Kubáň
Keyword(s):  
Flow System ◽  
Detection System ◽  
Film Formation ◽  
Liquid Extraction ◽  
Photometric Detection ◽  
Liquid Liquid Extraction ◽  
Teflon Tube ◽  
Continuous Stream ◽  
Computer Controlled ◽  
Segmented Volume

The behaviour of a thin film of an organic solvent on the walls of the extraction coil in a continuous liquid-liquid extraction flow system was studied using a computer-controlled fast-recording on-tube photometric detection system (approx. 3 ms time resolution). A single-loop injector was employed to introduce precise, reproducible volumes (Sr < 2%) of one phase into the continuous stream of the other as a segmented volume standard. The film thickness Df, ranging from 1 to 20 μm for a 0.7 mm teflon tube, was calculated from the segment lengthening at a different chloroform flow rates and was found to obey a polynominal dependence on the linear flow rate, df = f(uα), where α < 1.

scholarly journals Time-resolved detection of SDS-induced conformational changes in α-synuclein by a micro-stopped-flow system

RSC Advances ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 1086-1097
Author(s):  
Shunki Takaramoto ◽  
Yusuke Nakasone ◽  
Kei Sadakane ◽  
Shinsaku Maruta ◽  
Masahide Terazima
Keyword(s):  
Conformational Changes ◽  
Flow System ◽  
Stopped Flow ◽  
Time Resolved ◽  
Sds Micelles ◽  
Conformation Changes ◽  
System P

Dynamics of conformation changes of α-synuclein induced by the presence of SDS micelles are revealed using time-resolved diffusion, CD, and FRET measurements combined with a micro-stopped flow system.

Evaluation of a discrete samples/stopped-flow mixer system for equilibrium and kinetic analyses.

1977 ◽  
Vol 23 (7) ◽  
pp. 1230-1237 ◽  
Author(s):  
H L Pardue ◽  
H T Gaw ◽  
G E Mieling ◽  
V L Mathews ◽  
D M Fast ◽  
...  
Keyword(s):  
Kinetic Method ◽  
Rapid Change ◽  
Reliable Data ◽  
Stopped Flow ◽  
Sampling System ◽  
Flow Mixing ◽  
Instrumental System ◽  
Computer Controlled ◽  
Fast Kinetic ◽  
Slow Kinetic

Abstract This paper describes the evaluation of a system for computer-controlled discrete sampling and stopped-flow mixing for equilibrium and kinetic determinations of several sorts of analytes in human serum. The instrumental system features a wash-out sampling system that permits rapid change-over from one sample and (or) reagent type to another, and a mixing-measurement system that can provide reliable data as soon as 10 ms after reagent and sample are mixed. Examples discussed include equilibrium procedures for glucose and cholesterol, slow kinetic procedures for glucose and lactate dehydrogenase, and a fast kinetic method for thiocyanate. The regression equation for all stopped-flow results (n = 114) vs. results by conventional methods is y = (103 +/- 0.01)x - (0.016 +/- 0.019) for numerical values of y between 0.3 and 3.0. The correlation coefficient for these data was 0.991. These results demonstrate that the stopped-flow method is a viable analytical approach for equilibrium, slow kinetic, and fast kinetic determinations that require measurement times shorter than 0.1 s.

scholarly journals Kinetic Studies of the Interaction of Toluidine Blue with Poly-(αL-glutamic acid) by Means of Stopped-Flow Method

1978 ◽  
Vol 10 (2) ◽  
pp. 153-159 ◽  
Author(s):  
Hidetoshi Ushio ◽  
Hiroshi Uchimura ◽  
Yoshikuni Tsuji ◽  
Takayuki Sano ◽  
Tatsuya Yasunaga
Keyword(s):  
Glutamic Acid ◽  
Toluidine Blue ◽  
Kinetic Studies ◽  
Stopped Flow ◽  
Flow Method

scholarly journals A micro-volume adaptation of a stopped-flow system; use with μg quantities of muscle proteins

2019 ◽  
Vol 581 ◽  
pp. 113338
Author(s):  
J. Walklate ◽  
Zoltan Ujfalusi ◽  
Vincent Behrens ◽  
Edward J. King ◽  
Michael A. Geeves
Keyword(s):  
Flow System ◽  
Stopped Flow ◽  
Muscle Proteins ◽  
System Use

Kinetic studies of anthracycline-DNA interaction by fluorescence stopped flow confirm a complex association mechanism

Biochemistry ◽  
1989 ◽  
Vol 28 (1) ◽  
pp. 274-282 ◽  
Author(s):  
Vincenzo Rizzo ◽  
Nereo Sacchi ◽  
Milena Menozzi
Keyword(s):  
Kinetic Studies ◽  
Dna Interaction ◽  
Stopped Flow

Stopped-flow measurements of CO and O2 uptake by hemoglobin in sheep erythrocytes

1963 ◽  
Vol 18 (1) ◽  
pp. 158-165 ◽  
Author(s):  
John A. Sirs ◽  
F. J. W. Roughton
Keyword(s):  
Linear Relation ◽  
Flow System ◽  
Stopped Flow ◽  
Constant Flow ◽  
Dissolved Gas ◽  
O2 Uptake ◽  
Flow Measurements ◽  
Sheep Erythrocytes ◽  
Flow Method ◽  
Comparison Of The Results

A stopped-flow method is described to measure spectrophotometrically the rates of uptake of CO and O2 by the hemoglobin of intact sheep erythrocytes. A comparison of the results obtained with the constant flow system has led to further refinement of the latter procedure. A linear relation is found between the rate of uptake and the concentration of the dissolved gas, and the initial percentage rate of saturation is independent of the concentration of the erythrocytes. Submitted on September 21, 1961

Computer controlled stopped-flow studies. Application to simultaneous kinetic analyses

1972 ◽  
Vol 44 (12) ◽  
pp. 1934-1939 ◽  
Author(s):  
Donald. Sanderson ◽  
John A. Bittikofer ◽  
Harry L. Pardue
Keyword(s):  
Stopped Flow ◽  
Computer Controlled
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