How Ternary Mobile Phases Allow Tuning of Analyte Retention in Hydrophilic Interaction Liquid Chromatography

2013 ◽  
Vol 85 (18) ◽  
pp. 8850-8856 ◽  
Author(s):  
Sergey M. Melnikov ◽  
Alexandra Höltzel ◽  
Andreas Seidel-Morgenstern ◽  
Ulrich Tallarek
Keyword(s):  
Liquid Chromatography ◽  
Hydrophilic Interaction Liquid Chromatography ◽  
Hydrophilic Interaction ◽  
Mobile Phases ◽  
Ternary Mobile Phases

Hydrophilic Interaction Liquid Chromatography (HILIC): Latest Applications in the Pharmaceutical Researches

2020 ◽  
Vol 16 ◽  
Author(s):  
Cem Erkmen ◽  
Weldejeworgıs Hndeya Gebrehiwot ◽  
Bengi Uslu
Keyword(s):  
Liquid Chromatography ◽  
Stationary Phases ◽  
Pharmaceutical Research ◽  
Polar Compounds ◽  
Separation Method ◽  
Hydrophilic Interaction Liquid Chromatography ◽  
Animal Origin ◽  
Hydrophilic Interaction ◽  
Mobile Phases ◽  
Phase Liquid

Background: Significant advances have been occurred in analytical research since the 1970s by liquid chromatography (LC) as the separation method. Reverse phase liquid chromatography (RPLC) method, using hydrophobic stationary phases and polar mobile phases is the most commonly used chromatographic method. However, it is difficult to analyze some polar compounds with this method. Another separation method, normal phase liquid chromatography (NPLC), which involves polar stationary phases with organic eluents. NPLC presents low-efficiency separations and asymmetric chromatographic peak shapes when analyzing polar compounds. Hydrophilic interaction liquid chromatography (HILIC) is an interesting and promising alternative method for the analysis of polar compounds. HILIC is be defined a separation method which combines stationary phases used in the NPLC method and mobile phases used in RPLC method. HILIC can be successfully applied to all types of liquid chromatographic separations such as pharmaceutical compounds, small molecules, metabolites, drugs of abuse, carbohydrates, toxins, oligosaccharides, peptides, amino acids and proteins. Objective: This paper provides a general overview of the recent application of HILIC in the pharmaceutical research in the different sample matrices such as pharmaceutical dosage form, plasma, serum, environmental samples, animal origin samples, plant origin samples, etc. Also, this review focuses on the most recent and selected papers in the drug research from 2009 to submission date in 2020 dealing with the analysis of different components using HILIC. Results and Conclusion: The literature survey showed that HILIC applications are increasing every year in pharmaceutical research. It was found that HILIC allows simultaneous analysis of many compounds using different detectors.

scholarly journals ANALYSIS OF THE RESULTS OF USING AMINO, AMIDE AND NITRILE SORBENTS IN HYDROPHILIC INTERACTION LIQUID CHROMATOGRAPHY

2018 ◽  
Vol 8 (3) ◽  
pp. 162-170
Author(s):  
A. S. Osipov ◽  
O. A. Popova ◽  
S. G. Larionova
Keyword(s):  
Liquid Chromatography ◽  
Hydrophilic Interaction Liquid Chromatography ◽  
Hydroxyl Groups ◽  
Hydroxybenzoic Acid ◽  
Hydrophilic Interaction ◽  
Nitrate Esters ◽  
Mobile Phases ◽  
Amino Amide ◽  
Analysis Of Results ◽  
Hydrophilic Compounds

The article summarises the results of studies of hydrophilic  interaction  liquid chromatography performed  from 2013 until 2017. The analysis of results obtained  during separation  of a number  of model mixtures of compounds  having different nature  helped to compare  the characteristics  of columns  packed with amino,  amide and nitrile sorbents. It was demonstrated that columns packed with amino sorbents could be used for the analysis of both hydrophilic compounds  and some hydrophobic compounds  containing acidic (4-hydroxybenzoic acid) and potentially acidic groups (nitro groups of nitro compounds;  phenolic hydroxyl groups of butylhydroxyanisole isomers and parabens). It was discovered that chromatographic columns packed with nitrile and amide sorbents have common properties and can be used for the analysis of hydrophilic compounds (hydroxycarbamide) and platinum coordination compounds, but cannot be used for the analysis of butylhydroxyanisole isomers, esters of p-hydroxybenzoic acid and nitrate  esters of isosorbide. The article offers hypotheses about possible mechanisms  of interaction  between test substances and sorbents. It was concluded  that the increase in the acetonitrile  content  in mobile phases, which increases the retention time of the test substances and results in better resolution between their peaks, illustrates the mechanism  of hydrophilic interaction  liquid chromatography.

Carboxylated cyclofructan 6 as a hydrolytically stable high efficiency stationary phase for hydrophilic interaction liquid chromatography and mixed mode separations

2016 ◽  
Vol 8 (31) ◽  
pp. 6038-6045 ◽  
Author(s):  
Yadi Wang ◽  
M. Farooq Wahab ◽  
Zachary S. Breitbach ◽  
Daniel W. Armstrong
Keyword(s):  
Liquid Chromatography ◽  
Benzoic Acid ◽  
Stationary Phase ◽  
Mixed Mode ◽  
High Efficiency ◽  
Stationary Phases ◽  
Hydrophilic Interaction Liquid Chromatography ◽  
Hydrophilic Interaction

Stationary phases composed of native cyclofructan 6 (CF6) and benzoic acid modified CF6 were synthesized and evaluated for hydrophilic interaction liquid chromatography (HILIC).

Aminoquinolines as fluorescent labels for hydrophilic interaction liquid chromatography of oligosaccharides

2012 ◽  
Vol 393 (8) ◽  
pp. 757-765 ◽  
Author(s):  
Weston B. Struwe ◽  
Pauline M. Rudd
Keyword(s):  
Liquid Chromatography ◽  
Hplc Analysis ◽  
Three Dimensional ◽  
Hydrophilic Interaction Liquid Chromatography ◽  
Peak Capacity ◽  
Fluorescent Labels ◽  
Sample Analysis ◽  
Hydrophilic Interaction ◽  
Optimal Excitation ◽  
Complex Sample

Abstract In this study, we investigated the potential of four different aminoquinoline (AQ) compounds as fluorescent labels for glycan analysis using hydrophilic interaction liquid chromatography (HILIC) and fluorescence detection (FLD). We confirmed the optimal excitation and emission wavelengths of 3-AQ and 6-AQ conjugated to glycan standards using three-dimensional fluorescent spectral scanning. The optimal excitation and emission wavelengths for 6-AQ were confirmed at λex=355 nm and λem=440 nm. We concluded that the optimal wavelengths for 3-AQ were λex=355 nm and λem=420 nm, which differed considerably from the wavelengths applied in previous reports. HILIC-FLD chromatograms using experimentally determined wavelengths were similar to 2-aminobenzamide controls, but the peak capacity and resolution differed significantly when published 3-AQ λex/em values were applied. Furthermore, we found that 5-AQ and 8-AQ labeled maltohexaose did not display any fluorescent pro\xadperties when used as a carbohydrate tag for HPLC analysis. Finally, we applied experimentally determined wavelengths to 3-AQ labeled N-glycans released from human IgG to illustrate changes in retention time as well as to demonstrate that AQ labeling is applicable to complex sample analysis via exoglycosidase sequencing.

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