Chemical Library Screening Using a SPR-Based Inhibition in Solution Assay: Simulations and Experimental Validation

2013 ◽  
Vol 85 (18) ◽  
pp. 8787-8795 ◽  
Author(s):  
Laurence Choulier ◽  
Yves Nominé ◽  
Gabrielle Zeder-Lutz ◽  
Sebastian Charbonnier ◽  
Bruno Didier ◽  
...  
Keyword(s):  
Experimental Validation ◽  
Library Screening ◽  
Chemical Library

In Silico Chemical Library Screening and Experimental Validation of a Novel 9-Aminoacridine Based Lead-Inhibitor of Human S-Adenosylmethionine Decarboxylase.

ChemInform ◽  
2007 ◽  
Vol 38 (49) ◽  
Author(s):  
Wesley H. Brooks ◽  
Diane E. McCloskey ◽  
Kenyon G. Daniel ◽  
Steven E. Ealick ◽  
John A. III Secrist ◽  
...  
Keyword(s):  
In Silico ◽  
Experimental Validation ◽  
Library Screening ◽  
Chemical Library ◽  
Adenosylmethionine Decarboxylase

scholarly journals In silico chemical library screening and experimental validation of novel compounds with potential varroacide activities

2019 ◽  
Vol 160 ◽  
pp. 11-19
Author(s):  
Clémence Riva ◽  
Peggy Suzanne ◽  
Gaël Charpentier ◽  
Fabienne Dulin ◽  
Marie-Pierre Halm-Lemeille ◽  
...  
Keyword(s):  
In Silico ◽  
Experimental Validation ◽  
Library Screening ◽  
Chemical Library

In Silico Chemical Library Screening and Experimental Validation of a Novel 9-Aminoacridine Based Lead-Inhibitor of Human S-Adenosylmethionine Decarboxylase

2007 ◽  
Vol 47 (5) ◽  
pp. 1897-1905 ◽  
Author(s):  
Wesley H. Brooks ◽  
Diane E. McCloskey ◽  
Kenyon G. Daniel ◽  
Steven E. Ealick ◽  
John A. Secrist ◽  
...  
Keyword(s):  
In Silico ◽  
Experimental Validation ◽  
Library Screening ◽  
Chemical Library ◽  
Adenosylmethionine Decarboxylase

Chemical library screening for WNK signalling inhibitors using fluorescence correlation spectroscopy

2013 ◽  
Vol 455 (3) ◽  
pp. 339-345 ◽  
Author(s):  
Takayasu Mori ◽  
Eriko Kikuchi ◽  
Yuko Watanabe ◽  
Shinya Fujii ◽  
Mari Ishigami-Yuasa ◽  
...  
Keyword(s):  
High Throughput ◽  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Library Screening ◽  
Chemical Library ◽  
Fluorescence Correlation ◽  
Fluorescent Correlation Spectroscopy

To discover WNK–OSR1/SPAK signalling inhibitors, we generated a new high-throughput system using fluorescent correlation spectroscopy capable of screening compounds that disrupt the binding of two molecules. We finally identified two novel and promising compounds for WNK–OSR1/SPAK signalling inhibition.

scholarly journals Phenotype-based high-content chemical library screening identifies statins as inhibitors of in vivo lymphangiogenesis

2012 ◽  
Vol 109 (40) ◽  
pp. E2665-E2674 ◽  
Author(s):  
M. M. P. Schulz ◽  
F. Reisen ◽  
S. Zgraggen ◽  
S. Fischer ◽  
D. Yuen ◽  
...  
Keyword(s):  
Library Screening ◽  
Chemical Library

scholarly journals Front Cover: Development and Validation of 2D Difference Intensity Analysis for Chemical Library Screening by Protein-Detected NMR Spectroscopy (ChemBioChem 5/2018)

ChemBioChem ◽  
2018 ◽  
Vol 19 (5) ◽  
pp. 419-419
Author(s):  
John M. Egner ◽  
Davin R. Jensen ◽  
Michael D. Olp ◽  
Nolan W. Kennedy ◽  
Brian F. Volkman ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
Library Screening ◽  
Intensity Analysis ◽  
Chemical Library ◽  
Front Cover ◽  
Development And Validation

scholarly journals Multiplexed experimental strategies for fragment library screening using SPR biosensors

2020 ◽  
Author(s):  
Edward A. FitzGerald ◽  
Darius Vagrys ◽  
Giulia Opassi ◽  
Hanna F. Klein ◽  
David J. Hamilton ◽  
...  
Keyword(s):  
Library Screening ◽  
Lead Discovery ◽  
Chemical Library ◽  
Innovative Strategies ◽  
Spr Biosensor ◽  
Screening Experiments ◽  
Fragment Library ◽  
Experimental Approaches ◽  
Orthogonal Methods ◽  
Experimental Strategies

AbstractSurface plasmon resonance biosensor technology (SPR) is ideally suited for fragment-based lead discovery. However, generally suitable experimental procedures or detailed protocols are lacking, especially for structurally or physico-chemically challenging targets or when tool compounds are lacking. Success depends on accounting for the features of both the target and the chemical library, purposely designing screening experiments for identification and validation of hits with desired specificity and mode-of-action, and availability of orthogonal methods capable of confirming fragment hits. By adopting a multiplexed strategy, the range of targets and libraries amenable to an SPR biosensor-based approach for identifying hits is considerably expanded. We here illustrate innovative strategies using five challenging targets and variants thereof. Two libraries of 90 and 1056 fragments were screened using two different flow-based SPR biosensor systems, allowing different experimental approaches. Practical considerations and procedures accounting for the characteristics of the proteins and libraries, and that increase robustness, sensitivity, throughput and versatility are highlighted.

scholarly journals Development and Validation of 2D Difference Intensity Analysis for Chemical Library Screening by Protein-Detected NMR Spectroscopy

ChemBioChem ◽  
2018 ◽  
Vol 19 (5) ◽  
pp. 448-458 ◽  
Author(s):  
John M. Egner ◽  
Davin R. Jensen ◽  
Michael D. Olp ◽  
Nolan W. Kennedy ◽  
Brian F. Volkman ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
Library Screening ◽  
Intensity Analysis ◽  
Chemical Library ◽  
Development And Validation

scholarly journals Identification of Three Classes of Heteroaromatic Compounds with Activity against Intracellular Trypanosoma cruzi by Chemical Library Screening

2009 ◽  
Vol 3 (2) ◽  
pp. e384 ◽  
Author(s):  
Esther Bettiol ◽  
Marie Samanovic ◽  
Andrew S. Murkin ◽  
Jayne Raper ◽  
Frederick Buckner ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Library Screening ◽  
Chemical Library ◽  
Heteroaromatic Compounds
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