scholarly journals Microfluidic Array with Integrated Oxygenation Control for Real-Time Live-Cell Imaging: Effect of Hypoxia on Physiology of Microencapsulated Pancreatic Islets

2013 ◽  
Vol 85 (23) ◽  
pp. 11240-11249 ◽  
Author(s):  
Mohammad Nourmohammadzadeh ◽  
Joe F. Lo ◽  
Matt Bochenek ◽  
Joshua E. Mendoza-Elias ◽  
Qian Wang ◽  
...  
Keyword(s):  
Pancreatic Islets ◽  
Real Time ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell

scholarly journals A microfluidic array for real-time live-cell imaging of human and rodent pancreatic islets

Lab on a Chip ◽  
2016 ◽  
Vol 16 (8) ◽  
pp. 1466-1472 ◽  
Author(s):  
Mohammad Nourmohammadzadeh ◽  
Yuan Xing ◽  
Jin Wuk Lee ◽  
Matthew A. Bochenek ◽  
Joshua E. Mendoza-Elias ◽  
...  
Keyword(s):  
High Resolution ◽  
Pancreatic Islets ◽  
Real Time ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
High Resolution Imaging ◽  
Resolution Imaging

In this study, we present a microfluidic array for high-resolution imaging of individual pancreatic islets.

Real-time and quantitative fluorescent live-cell imaging with quadruplex-specific red-edge probe (G4-REP)

2017 ◽  
Vol 1861 (5) ◽  
pp. 1312-1320 ◽  
Author(s):  
Sunny Y. Yang ◽  
Souheila Amor ◽  
Aurélien Laguerre ◽  
Judy M.Y. Wong ◽  
David Monchaud
Keyword(s):  
Real Time ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Red Edge ◽  
Edge Probe

Single live cell imaging for real-time monitoring of resistance mechanism in Pseudomonas aeruginosa

2002 ◽  
Vol 7 (4) ◽  
pp. 576 ◽  
Author(s):  
Sophia V. Kyriacou ◽  
Michelle E. Nowak ◽  
William J. Brownlow ◽  
Xiao-Hong Nancy Xu
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Real Time ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Resistance Mechanism ◽  
Live Cell ◽  
Real Time Monitoring

Engineering a Reversible Fluorescent Probe for Real-Time Live-Cell Imaging and Quantification of Mitochondrial ATP

2020 ◽  
Vol 92 (6) ◽  
pp. 4681-4688 ◽  
Author(s):  
Tian-Bing Ren ◽  
Si-Yu Wen ◽  
Lu Wang ◽  
Peng Lu ◽  
Bin Xiong ◽  
...  
Keyword(s):  
Real Time ◽  
Fluorescent Probe ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell

scholarly journals A Fluorescent Real-Time Plaque Assay Enables Single-Cell Analysis of Virus-Induced Cytopathic Effect by Live-Cell Imaging

Viruses ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1193
Author(s):  
Jorge L. Arias-Arias ◽  
Eugenia Corrales-Aguilar ◽  
Rodrigo A. Mora-Rodríguez
Keyword(s):  
Image Analysis ◽  
Real Time ◽  
Crystal Violet ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Cytopathic Effect ◽  
Plaque Assay ◽  
Live Cell ◽  
Cell Permeability ◽  
Cytotoxic Activities

Conventional plaque assays rely on the use of overlays to restrict viral infection allowing the formation of distinct foci that grow in time as the replication cycle continues leading to countable plaques that are visualized with standard techniques such as crystal violet, neutral red, or immunolabeling. This classical approach takes several days until large enough plaques can be visualized and counted with some variation due to subjectivity in plaque recognition. Since plaques are clonal lesions produced by virus-induced cytopathic effect, we applied DNA fluorescent dyes with differential cell permeability to visualize them by live-cell imaging. We could observe different stages of that cytopathic effect corresponding to an early wave of cells with chromatin-condensation followed by a wave of dead cells with membrane permeabilization within plaques generated by different animal viruses. This approach enables an automated plaque identification using image analysis to increase single plaque resolution compared to crystal violet counterstaining and allows its application to plaque tracking and plaque reduction assays to test compounds for both antiviral and cytotoxic activities. This fluorescent real-time plaque assay sums to those next-generation technologies by combining this robust classical method with modern fluorescence microscopy and image analysis approaches for future applications in virology.

scholarly journals In Situ Spatial Complementation of Aptamer-Mediated Recognition Enables Live-Cell Imaging of Native RNA Transcripts in Real Time

2017 ◽  
Vol 57 (4) ◽  
pp. 972-976 ◽  
Author(s):  
Zejun Wang ◽  
Yao Luo ◽  
Xiaodong Xie ◽  
Xingjie Hu ◽  
Haiyun Song ◽  
...  
Keyword(s):  
Real Time ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Rna Transcripts
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