Maximizing Mismatch Discrimination by Surface-Tethered Locked Nucleic Acid Probes via Ionic Tuning

2013 ◽  
Vol 85 (3) ◽  
pp. 1615-1623 ◽  
Author(s):  
Sourav Mishra ◽  
Srabani Ghosh ◽  
Rupa Mukhopadhyay
Keyword(s):  
Nucleic Acid ◽  
Locked Nucleic Acid ◽  
Nucleic Acid Probes ◽  
Mismatch Discrimination

MicroRNA detection by northern blotting using locked nucleic acid probes

2008 ◽  
Vol 3 (2) ◽  
pp. 190-196 ◽  
Author(s):  
Éva Várallyay ◽  
József Burgyán ◽  
Zoltán Havelda
Keyword(s):  
Nucleic Acid ◽  
Northern Blotting ◽  
Locked Nucleic Acid ◽  
Nucleic Acid Probes ◽  
Microrna Detection

mRNA Quantification After Fluorescence Activated Cell Sorting Using Locked Nucleic Acid Probes

2011 ◽  
Vol 49 (1) ◽  
pp. 42-47 ◽  
Author(s):  
Rie Maruo ◽  
Hiroya Yamada ◽  
Mikio Watanabe ◽  
Yoh Hidaka ◽  
Yoshinori Iwatani ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Cell Sorting ◽  
Locked Nucleic Acid ◽  
Fluorescence Activated Cell Sorting ◽  
Nucleic Acid Probes ◽  
Mrna Quantification

Ultrasensitive SERS detection of highly homologous miRNAs by generating 3D organic-nanoclusters and a functionalized chip with locked nucleic acid probes

2018 ◽  
Vol 54 (95) ◽  
pp. 13431-13434 ◽  
Author(s):  
Mengting Zhu ◽  
Zhaomei Sun ◽  
Zhen Zhang ◽  
Shusheng Zhang
Keyword(s):  
Nucleic Acid ◽  
Locked Nucleic Acid ◽  
Nucleotide Difference ◽  
Single Nucleotide ◽  
Nucleic Acid Probes ◽  
Sers Detection ◽  
New Finding

Herein, a new finding is reported that 3D organic-nanoclusters (3DONs) with superior SERS properties as an original reporter could accurately and sensitively distinguish microRNAs (miRNAs) with highly similar sequences, even with a single-nucleotide difference.

scholarly journals Detection of mRNA in living cells by double-stranded locked nucleic acid probes

The Analyst ◽  
2013 ◽  
Vol 138 (17) ◽  
pp. 4777 ◽  
Author(s):  
Reza Riahi ◽  
Zachary Dean ◽  
Ting-Hsiang Wu ◽  
Michael A. Teitell ◽  
Pei-Yu Chiou ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Living Cells ◽  
Locked Nucleic Acid ◽  
Nucleic Acid Probes

Splice-switching efficiency and specificity for oligonucleotides with locked nucleic acid monomers

2008 ◽  
Vol 412 (2) ◽  
pp. 307-313 ◽  
Author(s):  
Peter Guterstam ◽  
Maria Lindgren ◽  
Henrik Johansson ◽  
Ulf Tedebark ◽  
Jesper Wengel ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Antisense Oligonucleotides ◽  
High Efficiency ◽  
Locked Nucleic Acid ◽  
Switching Activity ◽  
Mismatch Discrimination ◽  
Therapeutic Platform ◽  
Splicing Patterns ◽  
Switching Efficiency ◽  
Target Effects

The use of antisense oligonucleotides to modulate splicing patterns has gained increasing attention as a therapeutic platform and, hence, the mechanisms of splice-switching oligonucleotides are of interest. Cells expressing luciferase pre-mRNA interrupted by an aberrantly spliced β-globin intron, HeLa pLuc705, were used to monitor the splice-switching activity of modified oligonucleotides by detection of the expression of functional luciferase. It was observed that phosphorothioate 2′-O-methyl RNA oligonucleotides containing locked nucleic acid monomers provide outstanding splice-switching activity. However, similar oligonucleotides with several mismatches do not impede splice-switching activity which indicates a risk for off-target effects. The splice-switching activity is abolished when mismatches are introduced at several positions with locked nucleic acid monomers suggesting that it is the locked nucleic acid monomers that give rise to low mismatch discrimination to target pre-mRNA. The results highlight the importance of rational sequence design to allow for high efficiency with simultaneous high mismatch discrimination for splice-switching oligonucleotides and suggest that splice-switching activity is tunable by utilizing locked nucleic acid monomers.

scholarly journals Development and Evaluation of Novel Real-Time Reverse Transcription-PCR Assays with Locked Nucleic Acid Probes Targeting Leader Sequences of Human-Pathogenic Coronaviruses

2015 ◽  
Vol 53 (8) ◽  
pp. 2722-2726 ◽  
Author(s):  
Jasper Fuk-Woo Chan ◽  
Garnet Kwan-Yue Choi ◽  
Alan Ka-Lun Tsang ◽  
Kah-Meng Tee ◽  
Ho-Yin Lam ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
Reverse Transcription ◽  
Locked Nucleic Acid ◽  
Small Rna Sequencing ◽  
Nucleic Acid Probes ◽  
Reverse Transcription Pcr ◽  
Highly Sensitive ◽  
Pcr Assays ◽  
Human Coronaviruses

Based on findings in small RNA-sequencing (Seq) data analysis, we developed highly sensitive and specific real-time reverse transcription (RT)-PCR assays with locked nucleic acid probes targeting the abundantly expressed leader sequences of Middle East respiratory syndrome coronavirus (MERS-CoV) and other human coronaviruses. Analytical and clinical evaluations showed their noninferiority to a commercial multiplex PCR test for the detection of these coronaviruses.

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