scholarly journals Capillary Electrophoresis–SELEX Selection of Catalytic DNA Aptamers for a Small-Molecule Porphyrin Target

2013 ◽  
Vol 85 (3) ◽  
pp. 1525-1530 ◽  
Author(s):  
Jing Yang ◽  
Michael T. Bowser
Keyword(s):  
Capillary Electrophoresis ◽  
Small Molecule ◽  
Dna Aptamers ◽  
Catalytic Dna ◽  
Selection Of

A single-round selection of selective DNA aptamers for mammalian cells by polymer-enhanced capillary transient isotachophoresis

The Analyst ◽  
2017 ◽  
Vol 142 (21) ◽  
pp. 4030-4038 ◽  
Author(s):  
Kazuki Hirose ◽  
Maho Tsuchida ◽  
Hinako Asakura ◽  
Koji Wakui ◽  
Keitaro Yoshimoto ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Mammalian Cells ◽  
Dna Aptamer ◽  
Dna Aptamers ◽  
Aptamer Selection ◽  
Selection For ◽  
First Time ◽  
Selection Of

A single-round DNA aptamer selection for mammalian cells was successfully achieved for the first time using a capillary electrophoresis (CE)-based methodology.

In Vitro Selection of DNA Aptamers for a Small-Molecule Porphyrin by Gold Nanoparticle-Based SELEX

2019 ◽  
Vol 87 (7-8) ◽  
pp. 231-239 ◽  
Author(s):  
Wenjing Li ◽  
Yu Luo ◽  
Tian Gao ◽  
Luyan Yang ◽  
Jine Wang ◽  
...  
Keyword(s):  
Gold Nanoparticle ◽  
Small Molecule ◽  
In Vitro Selection ◽  
Dna Aptamers ◽  
Selection Of

Electrochemical SELEX Technique for the Selection of DNA Aptamers against the Small Molecule 11-Deoxycortisol

2019 ◽  
Vol 2 (6) ◽  
pp. 2624-2632 ◽  
Author(s):  
Shimaa Eissa ◽  
Ayesha Siddiqua ◽  
Raja Chinnappan ◽  
Mohammed Zourob
Keyword(s):  
Small Molecule ◽  
Dna Aptamers ◽  
Selection Of

scholarly journals Quantitative Characterization of Partitioning in Selection of DNA Aptamers for Protein Targets by Capillary Electrophoresis

2021 ◽  
Author(s):  
An T. H. Le ◽  
Tong Y. Wang ◽  
Svetlana M. Krylova ◽  
Stanislav S. Beloborodov ◽  
Sergey Krylov
Keyword(s):  
Molecular Weight ◽  
Capillary Electrophoresis ◽  
High Efficiency ◽  
Dna Aptamers ◽  
Experimental Conditions ◽  
Protein Targets ◽  
Protein Target ◽  
Two Parameters ◽  
Dna Binders ◽  
Selection Of

Partitioning of protein–DNA complexes from protein-unbound DNA is a key step in selection of DNA aptamers. Conceptually, the partitioning step is characterized by two parameters: transmittance for protein-bound DNA (binders) and transmittance for unbound DNA (nonbinders). Here we present the first study to reveal how these transmittances depend on experimental conditions; such studies are pivotal to the effective planning and control of selection. Our focus was capillary electrophoresis (CE) which is a partitioning approach of high efficiency. By combining a theoretical model and experimental data, we evaluated the dependence of transmittances of binders and nonbinders on the molecular weight of protein target in two modes of CE-based partitioning: nonequilibrium capillary electrophoresis of equilibrium mixtures (NECEEM) and ideal-filter capillary electrophoresis (IFCE). Our data suggest that as the molecular weight of the protein target decreases: (i) the transmittance for binders remains close to unity in NECEEM but decreases drastically in IFCE and (ii) the transmittance for nonbinders increases orders of magnitude in NECEEM but remains relatively stable at a very low level in IFCE. To determine the optimal CE conditions for a given size of protein target, a balance between transmittances of binders and nonbinder must be reached; such a balance would ensure the collection of binders of sufficient purity and quantity. We conclude that, as a rule of thumb, IFCE is preferable for large-size protein targets while NECEEM should be the method of choice for small-size protein targets

Selection of DNA aptamers recognizing small cell lung cancer using living cell-SELEX

The Analyst ◽  
2011 ◽  
Vol 136 (7) ◽  
pp. 1310 ◽  
Author(s):  
Takao Kunii ◽  
Shun-ichiro Ogura ◽  
Masayasu Mie ◽  
Eiry Kobatake
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Cell Lung Cancer ◽  
Living Cell ◽  
Small Cell ◽  
Dna Aptamers ◽  
Small Cell Lung ◽  
Selection Of

scholarly journals Selection of DNA Aptamers Recognizing EpCAM-Positive Prostate Cancer by Cell-SELEX for in vitro and in vivo MR Imaging

2021 ◽  
Vol Volume 15 ◽  
pp. 3985-3996
Author(s):  
Jinman Zhong ◽  
Jianke Ding ◽  
Lei Deng ◽  
Ying Xiang ◽  
Duoduo Liu ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Mr Imaging ◽  
Dna Aptamers ◽  
Selection Of
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