Detection of Pathogenic Bacteria by Using Zinc Finger Protein Fused with Firefly Luciferase

2012 ◽  
Vol 84 (18) ◽  
pp. 8028-8032 ◽  
Author(s):  
Koichi Abe ◽  
Takenori Kumagai ◽  
Chiaki Takahashi ◽  
Aki Kezuka ◽  
Yoshiyuki Murakami ◽  
...  
Keyword(s):  
Zinc Finger ◽  
Pathogenic Bacteria ◽  
Zinc Finger Protein ◽  
Firefly Luciferase ◽  
Finger Protein

Electrochemical detection of pathogenic bacteria by using a glucose dehydrogenase fused zinc finger protein

2014 ◽  
Vol 6 (14) ◽  
pp. 4991-4994 ◽  
Author(s):  
Jinhee Lee ◽  
Atsuro Tatsumi ◽  
Koichi Abe ◽  
Wataru Yoshida ◽  
Koji Sode ◽  
...  
Keyword(s):  
Electrochemical Detection ◽  
Zinc Finger ◽  
Genomic Dna ◽  
Pathogenic Bacteria ◽  
Zinc Finger Protein ◽  
Detection System ◽  
Glucose Dehydrogenase ◽  
Finger Protein ◽  
Pcr Products ◽  
Probe Hybridization

We developed an electrochemical detection system for pathogenic bacteria by utilizing a glucose dehydrogenase-fused zinc finger protein (ZF-GDH), which could detect PCR products electrochemically without the need for DNA probe hybridization. Using ZF-GDH, we could specifically detect 10 copies of genomic DNA derived fromEscherichia coliO157.

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