Quantitative Multianalyte Microarray Immunoassay Utilizing Upconverting Phosphor Technology

2012 ◽  
Vol 84 (20) ◽  
pp. 8628-8634 ◽  
Author(s):  
Henna Päkkilä ◽  
Minna Ylihärsilä ◽  
Satu Lahtinen ◽  
Liisa Hattara ◽  
Niina Salminen ◽  
...  
Keyword(s):  
Microarray Immunoassay

scholarly journals Rapid and simultaneous detection of ricin, staphylococcal enterotoxin B and saxitoxin by chemiluminescence-based microarray immunoassay

The Analyst ◽  
2014 ◽  
Vol 139 (22) ◽  
pp. 5885-5892 ◽  
Author(s):  
A. Szkola ◽  
E. M. Linares ◽  
S. Worbs ◽  
B. G. Dorner ◽  
R. Dietrich ◽  
...  
Keyword(s):  
Simultaneous Detection ◽  
Staphylococcal Enterotoxin ◽  
Staphylococcal Enterotoxin B ◽  
Microarray Immunoassay ◽  
Enterotoxin B ◽  
Idiotypic Antibody

Simultaneous detection of proteotoxins, ricin and SEB, and small toxin, STX, on a chemiluminescence-based microarray using anti-idiotypic antibody for STX.

scholarly journals Antibody Microarray Immunoassay for Simultaneous Quantification of Multiple Mycotoxins in Corn Samples

Toxins ◽  
2018 ◽  
Vol 10 (10) ◽  
pp. 415 ◽  
Author(s):  
Xian Zhang ◽  
Zuohuan Wang ◽  
Yun Fang ◽  
Renjie Sun ◽  
Tong Cao ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Aflatoxin B1 ◽  
Goodness Of Fit ◽  
Limit Of Detection ◽  
Simultaneous Detection ◽  
Fumonisin B1 ◽  
Quantitative Detection ◽  
Antibody Microarray ◽  
Test Kit ◽  
Microarray Immunoassay

We developed and tested a prototype of an antibody microarray immunoassay for simultaneous quantitative detection of four typical mycotoxins (aflatoxin B1, ochratoxin A, zearalenone, and fumonisin B1) in corn samples. The test kit consisted of a nitrocellulose membrane layered with immobilized monoclonal antibodies against mycotoxins. During the assay, the mycotoxin-protein conjugates were biotinylated. The signal detection was enhanced by a combination of the biotin-streptavidin system and enhanced chemiluminescence (ECL). This improved the sensitivity of the assay. Under the optimized conditions, four calibration curves with goodness of fit (R2 > 0.98) were plotted. The results showed that the detection limits for aflatoxin B1, ochratoxin A, zearalenone, and fumonisin B1 were 0.21, 0.19, 0.09, and 0.24 ng/mL, with detection ranges of 0.47–55.69, 0.48–127.11, 0.22–31.36, and 0.56–92.57 ng/mL, respectively. The limit of detection (LOD) of this antibody microarray for aflatoxin B1, ochratoxin A, zearalenone, and fumonisin B1 in corn was 5.25, 4.75, 2.25, and 6 μg/kg, respectively. The recovery rates from the spiked samples were between 79.2% and 113.4%, with coefficient of variation <10%. The results of the analysis of commercial samples for mycotoxins using this new assay and the liquid chromatography-tandem mass spectrometry (LC-MS/MS) were comparable and in good agreement. This assay could also be modified for the simultaneous detection of other multiple mycotoxins, as well as low-weight analytes, hazardous to human health.

Microarray immunoassay: Association of clinical history, in vitro IgE function, and heterogeneity of allergenic peanut epitopes

2004 ◽  
Vol 113 (4) ◽  
pp. 776-782 ◽  
Author(s):  
Wayne G. Shreffler ◽  
Kirsten Beyer ◽  
Te-Hua Tearina Chu ◽  
A.Wesley Burks ◽  
Hugh A. Sampson
Keyword(s):  
Clinical History ◽  
Microarray Immunoassay

Multiplexed infectious protein microarray immunoassay suitable for the study of the specificity of monoclonal immunoglobulins

2013 ◽  
Vol 433 (2) ◽  
pp. 202-209 ◽  
Author(s):  
Delphine Feron ◽  
Cathy Charlier ◽  
Victor Gourain ◽  
Laurent Garderet ◽  
Marianne Coste-Burel ◽  
...  
Keyword(s):  
Protein Microarray ◽  
Monoclonal Immunoglobulins ◽  
Microarray Immunoassay
Sign in / Sign up

Export Citation Format

Share Document