Simultaneous Monitoring of Protein Adsorption Kinetics Using a Quartz Crystal Microbalance and Field-Effect Transistor Integrated Device

2012 ◽  
Vol 84 (17) ◽  
pp. 7308-7314 ◽  
Author(s):  
Tatsuro Goda ◽  
Yasuhiro Maeda ◽  
Yuji Miyahara
Keyword(s):  
Protein Adsorption ◽  
Quartz Crystal Microbalance ◽  
Adsorption Kinetics ◽  
Field Effect ◽  
Field Effect Transistor ◽  
Quartz Crystal ◽  
Effect Transistor ◽  
Integrated Device

scholarly journals Label-Free Monitoring of Histone Acetylation Using Aptamer-Functionalized Field-Effect Transistor and Quartz Crystal Microbalance Sensors

Micromachines ◽  
2020 ◽  
Vol 11 (9) ◽  
pp. 820
Author(s):  
Tatsuro Goda ◽  
Yuji Miyahara
Keyword(s):  
Histone Acetylation ◽  
Quartz Crystal Microbalance ◽  
Field Effect ◽  
Field Effect Transistor ◽  
Quartz Crystal ◽  
Accurate Diagnosis ◽  
Label Free ◽  
Post Translational Modification ◽  
Adsorbed Mass ◽  
Effect Transistor

Chemical and enzymatic modifications of amino acid residues in protein after translation contain rich information about physiological conditions and diseases. Histone acetylation/deacetylation is the essential post-translational modification by regulating gene transcription. Such qualitative changes of biomacromolecules need to be detected in point-of-care systems for an early and accurate diagnosis. However, there is no technique to aid this issue. Previously, we have applied an aptamer-functionalized field-effect transistor (FET) to the specific protein biosensing. Quantitative changes of target protein in a physiological solution have been determined by detecting innate charges of captured protein at the gate-solution interface. Moreover, we have succeeded in developing an integrated system of FET and quartz crystal microbalance (QCM) sensors for determining the adsorbed mass and charge, simultaneously or in parallel. Prompted by this, in this study, we developed a new label-free method for detecting histone acetylation using FET and QCM sensors. The loss of positive charge of lysine residue by chemically induced acetylation of histone subunits (H3 and H4) was successfully detected by potentiometric signals using anti-histone aptamer-functionalized FET. The adsorbed mass was determined by the same anti-histone aptamer-functionalized QCM. From these results, the degree of acetylation was correlated to the charge-to-mass ratio of histone subunits. The histone required for the detection was below 100 nM, owing to the high sensitivity of aptamer-functionalized FET and QCM sensors. These findings will guide us to a new way of measuring post-translational modification of protein in a decentralized manner for an early and accurate diagnosis.

Relation between Effective Charge Numbers and Signals Caused by Protein Adsorption on Field Effect Transistor Detection

2019 ◽  
Vol 35 (7) ◽  
pp. 121-124 ◽  
Author(s):  
Sho Hideshima ◽  
Takahiro Nakamura ◽  
Shigeki Kuroiwa ◽  
Tetsuya Osaka
Keyword(s):  
Protein Adsorption ◽  
Effective Charge ◽  
Field Effect ◽  
Field Effect Transistor ◽  
Effect Transistor

Charge-independent protein adsorption characteristics of epitaxial graphene field-effect transistor on SiC substrate

2021 ◽  
Vol 130 (7) ◽  
pp. 074502
Author(s):  
Hiroki Nakai ◽  
Daiu Akiyama ◽  
Yoshiaki Taniguchi ◽  
Iori Kishinobu ◽  
Hiromichi Wariishi ◽  
...  
Keyword(s):  
Protein Adsorption ◽  
Field Effect ◽  
Field Effect Transistor ◽  
Epitaxial Graphene ◽  
Adsorption Characteristics ◽  
Graphene Field Effect Transistor ◽  
Effect Transistor ◽  
Independent Protein
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