Enhancing the Power of Liquid Chromatography–Mass Spectrometry-Based Urine Metabolomics in Negative Ion Mode by Optimization of the Additive

2012 ◽  
Vol 84 (18) ◽  
pp. 7785-7792 ◽  
Author(s):  
Xumin Zhang ◽  
Morten Rahr Clausen ◽  
Xiaolu Zhao ◽  
Hong Zheng ◽  
Hanne Christine Bertram
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Negative Ion ◽  
Liquid Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Negative Ion Mode ◽  
Urine Metabolomics

High-sensitivity negative-ion laser spray for liquid chromatography/mass spectrometry

2001 ◽  
Vol 15 (21) ◽  
pp. 2020-2026 ◽  
Author(s):  
Kenzo Hiraoka ◽  
Yoshiki Asakawa ◽  
Yasuteru Yamamoto ◽  
Masana Nakamura ◽  
Kazuhiro Ueda
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
High Sensitivity ◽  
Negative Ion ◽  
Liquid Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Ion Laser

scholarly journals Determination of Chloramphenicol Residues in Milk, Eggs, and Tissues by Liquid Chromatography/Mass Spectrometry

2005 ◽  
Vol 88 (2) ◽  
pp. 645-653 ◽  
Author(s):  
Lisa Penney ◽  
Anderson Smith ◽  
Brent Coates ◽  
Arosha Wijewickreme
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Reversed Phase ◽  
Negative Ion ◽  
Liquid Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Relative Standard ◽  
The Matrix ◽  
Working Day

Abstract A new liquid chromatography/mass spectrometry (LC/MS) method is presented for the determination of chloramphenicol (CAP) residues in milk, eggs, chicken muscle and liver, and beef muscle and kidney. CAP is extracted from the samples with acetonitrile and defatted with hexane. The acetonitrile extracts are then evaporated, and residues are reconstituted in 10mM ammonium acetate–acetonitrile mobile phase and injected into the LC system. CAP is determined by reversed-phase chromatography using an Inertsil ODS-2 column and MS detection with negative ion electrospray ionization. Calibration curves were linear between 0.5–5.0 ng/g for all matrixes studied. The relative standard deviations for measurements by this method were generally <12%, and average recoveries ranged from 80 to 120%, depending on the matrix involved. The method detection limits of CAP ranged from 0.2 to 0.6 ng/g, which are comparable to previously reported results. The proposed method is rapid, simple, and specific, allowing a single analyst to easily prepare over 40 samples in a regular working day.

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