Small-Molecule-Based Affinity Chromatography Method for Antibody Purification via Nucleotide Binding Site Targeting

2012 ◽  
Vol 84 (18) ◽  
pp. 7721-7728 ◽  
Author(s):  
Nathan J. Alves ◽  
Samuel D. Stimple ◽  
Michael W. Handlogten ◽  
Jonathan D. Ashley ◽  
Tanyel Kiziltepe ◽  
...  
Keyword(s):  
Affinity Chromatography ◽  
Binding Site ◽  
Small Molecule ◽  
Nucleotide Binding ◽  
Nucleotide Binding Site ◽  
Chromatography Method ◽  
Antibody Purification ◽  
Site Targeting

scholarly journals Antibody purification via affinity membrane chromatography method utilizing nucleotide binding site targeting with a small molecule

The Analyst ◽  
2016 ◽  
Vol 141 (24) ◽  
pp. 6571-6582 ◽  
Author(s):  
Nur Mustafaoglu ◽  
Tanyel Kiziltepe ◽  
Basar Bilgicer
Keyword(s):  
Affinity Chromatography ◽  
Binding Site ◽  
Small Molecule ◽  
Nucleotide Binding ◽  
Nucleotide Binding Site ◽  
Chromatography Method ◽  
Antibody Purification ◽  
Membrane Affinity ◽  
Affinity Membrane ◽  
Site Targeting

m-NBST is a small-molecule based membrane affinity chromatography system that utilizes the NBS, providing high levels of antibody recovery and purity.

Characterization of Nucleotide Binding ­Site-Encoding Genes in Sweetpotato, Ipomoea batatas(L.) Lam., and Their Response to Biotic and Abiotic Stresses

2021 ◽  
pp. 1-15
Author(s):  
Zengzhi Si ◽  
Yake Qiao ◽  
Kai Zhang ◽  
Zhixin Ji ◽  
Jinling Han
Keyword(s):  
Binding Site ◽  
Abiotic Stresses ◽  
Ipomoea Batatas ◽  
Expression Profiles ◽  
Nucleotide Binding ◽  
Regulatory Elements ◽  
Nucleotide Binding Site ◽  
Biotic And Abiotic Stresses ◽  
Encoding Genes

Sweetpotato, <i>Ipomoea batatas</i> (L.) Lam., is an important and widely grown crop, yet its production is affected severely by biotic and abiotic stresses. The nucleotide binding site (NBS)-encoding genes have been shown to improve stress tolerance in several plant species. However, the characterization of NBS-encoding genes in sweetpotato is not well-documented to date. In this study, a comprehensive analysis of NBS-encoding genes has been conducted on this species by using bioinformatics and molecular biology methods. A total of 315 NBS-encoding genes were identified, and 260 of them contained all essential conserved domains while 55 genes were truncated. Based on domain architectures, the 260 NBS-encoding genes were grouped into 6 distinct categories. Phylogenetic analysis grouped these genes into 3 classes: TIR, CC (I), and CC (II). Chromosome location analysis revealed that the distribution of NBS-encoding genes in chromosomes was uneven, with a number ranging from 1 to 34. Multiple stress-related regulatory elements were detected in the promoters, and the NBS-encoding genes’ expression profiles under biotic and abiotic stresses were obtained. According to the bioinformatics analysis, 9 genes were selected for RT-qPCR analysis. The results revealed that <i>IbNBS75</i>, <i>IbNBS219</i>, and <i>IbNBS256</i> respond to stem nematode infection; <i>Ib­NBS240</i>, <i>IbNBS90</i>, and <i>IbNBS80</i> respond to cold stress, while <i>IbNBS208</i>, <i>IbNBS71</i>, and <i>IbNBS159</i> respond to 30% PEG treatment. We hope these results will provide new insights into the evolution of NBS-encoding genes in the sweetpotato genome and contribute to the molecular breeding of sweetpotato in the future.

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