scholarly journals Nonlinear Analyte Concentration Gradients for One-Step Kinetic Analysis Employing Optical Microring Resonators

2012 ◽  
Vol 84 (13) ◽  
pp. 5556-5564 ◽  
Author(s):  
Michael T. Marty ◽  
Courtney D. Kuhnline Sloan ◽  
Ryan C. Bailey ◽  
Stephen G. Sligar
Keyword(s):  
Kinetic Analysis ◽  
Microring Resonators ◽  
Analyte Concentration ◽  
Concentration Gradients ◽  
One Step

One-step immunoassays for free (unbound) hormones: the effect of tracer binding by serum proteins.

1986 ◽  
Vol 32 (1) ◽  
pp. 45-49 ◽  
Author(s):  
D Geiseler ◽  
P Chodha ◽  
R Ekins
Keyword(s):  
Serum Proteins ◽  
Single Step ◽  
Assay System ◽  
Measuring System ◽  
Binding Potential ◽  
Protein Distribution ◽  
Analyte Concentration ◽  
Binding Assays ◽  
One Step

Abstract In binding assays for determination of free (non-protein-bound) hormones in serum or plasma, the influence of the measuring system on the original analyte concentration in the sample must be considered. In one- or single-step free-hormone immunoassays, the labeled analyte or analog-tracer not only is bound to the antibody, it also is bound, to some extent, to serum proteins. The dependence of the assay response on two unknown variables--the concentration of free analyte and the binding potential of serum for the tracer--introduces a bias between the actual (original) and measured hormone concentrations. The significance of this protein effect is described by mathematical modeling of the analyte-protein distribution in the assay system. The theoretical consideration is validated by a clearly defined one-step assay system for measurement of free-thyroxin concentration, with a labeled thyroxin-immunoglobulin conjugate used as tracer.

One-step kinetic analysis of competitive growth of Salmonella spp. and background flora in ground chicken

Food Control ◽  
2020 ◽  
Vol 117 ◽  
pp. 107103
Author(s):  
Zhen Jia ◽  
Yabo Peng ◽  
Xiaotong Yan ◽  
Ziye Zhang ◽  
Ting Fang ◽  
...  
Keyword(s):  
Kinetic Analysis ◽  
Competitive Growth ◽  
Salmonella Spp ◽  
One Step

Kinetic Analysis of One-Step Solid-State Reaction for Li4Ti5O12/C

2011 ◽  
Vol 115 (46) ◽  
pp. 13413-13419 ◽  
Author(s):  
Xuebu Hu ◽  
Ziji Lin ◽  
Kerun Yang ◽  
Zhenghua Deng
Keyword(s):  
Solid State ◽  
Kinetic Analysis ◽  
Solid State Reaction ◽  
One Step

Assessing the growth of Listeria monocytogenes in salmon with or without the competition of background microflora -- A one-step kinetic analysis

Food Control ◽  
2020 ◽  
Vol 114 ◽  
pp. 107139
Author(s):  
Zhen Jia ◽  
Weijuan Bai ◽  
Xiaoting Li ◽  
Ting Fang ◽  
Changcheng Li
Keyword(s):  
Listeria Monocytogenes ◽  
Kinetic Analysis ◽  
One Step

Exponential Concentration Gradients in Microfluidic Devices for Cell Studies

2011 ◽  
Author(s):  
Šeila Selimović ◽  
Woo Young Sim ◽  
Sang Bok Kim ◽  
Yun Ho Jang ◽  
Won Gu Lee ◽  
...  
Keyword(s):  
Protein Crystallization ◽  
Experimental Studies ◽  
Cellular Responses ◽  
Analyte Concentration ◽  
Concentration Gradients ◽  
Cell Microenvironment ◽  
Biological Responses ◽  
Native Cell ◽  
High Throughput Assay ◽  
Concentration Levels

Microscale technologies are a powerful tool in many biological and chemical applications, as they utilize only small reagent volumes. Microfluidics is especially well compatible with biological materials and applications, for example protein crystallization, high throughput assay analysis, and various cell studies. In that context, non-linear gradients of particles and molecules as well as efficient mixing of the components inside the lab-on-a-chip are crucial for many experimental studies: testing of and analyzing biological responses to different analyte concentration levels, studying the native cell microenvironment or cellular responses during different growth and proliferation stages. Thus, a microfluidic approach that allows for generation of different concentration gradients and specifically exponential gradients emerges as a helpful technology, and is also compatible with cells.

scholarly journals Layered Double Hydroxide-Modified Organic Electrochemical Transistor for Glucose and Lactate Biosensing

Sensors ◽  
2020 ◽  
Vol 20 (12) ◽  
pp. 3453 ◽  
Author(s):  
Isacco Gualandi ◽  
Marta Tessarolo ◽  
Federica Mariani ◽  
Danilo Arcangeli ◽  
Luca Possanzini ◽  
...  
Keyword(s):  
Layered Double Hydroxide ◽  
Limit Of Detection ◽  
Drain Current ◽  
Analyte Concentration ◽  
Lactate Oxidase ◽  
Amperometric Response ◽  
Electrochemical Transistor ◽  
One Step ◽  
Double Hydroxide ◽  
Biosensor Response

Biosensors based on Organic Electrochemical Transistors (OECTs) are developed for the selective detection of glucose and lactate. The transistor architecture provides signal amplification (gain) with respect to the simple amperometric response. The biosensors are based on a poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS) channel and the gate electrode is functionalised with glucose oxidase (GOx) or lactate oxidase (LOx) enzymes, which are immobilised within a Ni/Al Layered Double Hydroxide (LDH) through a one-step electrodeposition procedure. The here-designed OECT architecture allows minimising the required amount of enzyme during electrodeposition. The output signal of the biosensor is the drain current (Id), which decreases as the analyte concentration increases. In the optimised conditions, the biosensor responds to glucose in the range of 0.1–8.0 mM with a limit of detection (LOD) of 0.02 mM. Two regimes of proportionality are observed. For concentrations lower than 1.0 mM, a linear response is obtained with a mean gain of 360, whereas for concentrations higher than 1.0 mM, Id is proportional to the logarithm of glucose concentration, with a gain of 220. For lactate detection, the biosensor response is linear in the whole concentration range (0.05–8.0 mM). A LOD of 0.04 mM is reached, with a net gain equal to 400.

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