Development of a Secondary Antibody Thio-Functionalized Microcantilever Immunosensor and an ELISA for Measuring Ginsenoside Re Content in the Herb Ginseng

2012 ◽  
Vol 84 (10) ◽  
pp. 4327-4333 ◽  
Author(s):  
Tiegui Nan ◽  
Shangquan Wu ◽  
Hongwei Zhao ◽  
Weiming Tan ◽  
Zhaohu Li ◽  
...  
Keyword(s):  
Secondary Antibody ◽  
Ginsenoside Re

A web-like network of type vi collagen in human skin is revealed by immuno-electron microscopy

1988 ◽  
Vol 46 ◽  
pp. 382-383
Author(s):  
Douglas R. Keene ◽  
Robert W. Glanville ◽  
Eva Engvall
Keyword(s):  
Electron Microscopy ◽  
Monoclonal Antibody ◽  
Human Skin ◽  
Basement Membranes ◽  
Primary Antibody ◽  
Fat Cells ◽  
Secondary Antibody ◽  
Type Vi Collagen ◽  
Dermal Matrix ◽  
Immuno Electron Microscopy

A mouse monoclonal antibody (5C6) prepared against human type VI collagen (1) has been used in this study to immunolocalize type VI collagen in human skin. The enbloc method used involves exposing whole tissue pieces to primary antibody and 5 nm gold conjugated secondary antibody before fixation, and has been described in detail elsewhere (2).Biopsies were taken from individuals ranging in age from neonate to 65 years old. By immuno-electron microscopy, type VI collagen is found to be distributed as a fine branching network closely associated with (but not attached to) banded collagen fibrils containing types I and III collagen (Fig. 1). It appears to enwrap fibers, to weave between individual fibrils within a fiber, and to span the distance separating fibers, creating a “web-like network” which entraps fibers within deep papillary and reticular dermal layers (Fig. 2). Relative to that in the dermal matrix, the concentration of type VI collagen is higher around endothelial basement membranes limiting the outer boundaries of nerves, capillaries, and fat cells (Fig. 3).

Regulation of the in vitro secondary antibody response

1980 ◽  
Vol 55 (2) ◽  
pp. 302-311 ◽  
Author(s):  
Sharyn M. Walker ◽  
William O. Weigle
Keyword(s):  
Antibody Response ◽  
Secondary Antibody

scholarly journals Changes in intestinal microbiota affect metabolism of ginsenoside re

2021 ◽  
Author(s):  
Lei Zhang ◽  
Fei Li ◽  
Wang‐Jun Qin ◽  
Chao Fu ◽  
Xiang‐Lin Zhang
Keyword(s):  
Intestinal Microbiota ◽  
Ginsenoside Re

scholarly journals IMMUNOLOGIC TOLERANCE AFTER SPECIFIC IMMUNIZATION

1964 ◽  
Vol 120 (3) ◽  
pp. 435-447 ◽  
Author(s):  
Marianne M. Dorner ◽  
Jonathan W. Uhr
Keyword(s):  
Human Serum Albumin ◽  
Bovine Serum Albumin ◽  
Antibody Response ◽  
Serum Albumin ◽  
Human Serum ◽  
Bovine Serum ◽  
The State ◽  
Immunologic Tolerance ◽  
Secondary Antibody

Specific immunologic tolerance to bovine serum albumin (BSA) was induced in approximately one-half of the rabbits that had been primarily immunized and were prepared for a secondary antibody response to BSA. The state of tolerance lasted for several months in the majority of rabbits and was not easily terminated by immunization with human serum albumin followed by BSA.

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