Magnetic Bead-Sensing-Platform-Based Chemiluminescence Resonance Energy Transfer and Its Immunoassay Application

2012 ◽  
Vol 84 (6) ◽  
pp. 2708-2712 ◽  
Author(s):  
Guoxin Qin ◽  
Shulin Zhao ◽  
Yong Huang ◽  
Jing Jiang ◽  
Fanggui Ye
Chemosensors ◽  
2019 ◽  
Vol 7 (3) ◽  
pp. 34 ◽  
Author(s):  
Gabr ◽  
Ibrahim ◽  
Tripathi ◽  
Prabhakar

A coumarin-benzothiazole ratiometric probe of ATP was designed and synthesized. The probe is based on incorporation of benzothiazole scaffold as a donor and coumarin nucleus as an acceptor in a single Förster resonance energy transfer/fluorescence resonance energy transfer (FRET) sensing platform. The sensor can detect ATP in aqueous solution with high selectivity over other nucleotide polyphosphate (NPP) anions. Binding of ATP to the sensor results in modulation of FRET efficiency between the donor and the acceptor which afforded a linear relationship between FRET signal and ATP (0.1–10 μM). A limit of detection (LOD) of 94.5 nM was quantified for FRET sensing of ATP by the probe. In addition, Job plot analysis revealed 1:1 binding interaction between the probe and ATP. The FRET probe was successfully utilized in monitoring ATP hydrolysis by apyrase in aqueous solution.


The Analyst ◽  
2020 ◽  
Vol 145 (10) ◽  
pp. 3535-3542 ◽  
Author(s):  
Jiaxue Gao ◽  
Hua Zhang ◽  
Zhenxin Wang

A DNA tetrahedron nanoprobe (DTNP)-based sensing platform for sensitive and selective detection of miRNA via FRET with DNA assisted amplification.


The Analyst ◽  
2018 ◽  
Vol 143 (3) ◽  
pp. 761-767 ◽  
Author(s):  
Lin Liu ◽  
Hua Zhang ◽  
Daqian Song ◽  
Zhenxin Wang

An upconversion nanoparticle-based fluorescence resonance energy transfer sensing platform has been developed for the caspase-3 activity detection in vitro and in cells.


Sign in / Sign up

Export Citation Format

Share Document