Combining Fourier Transform-Ion Cyclotron Resonance/Mass Spectrometry Analysis and Kendrick Plots for Silicon Speciation and Molecular Characterization in Petroleum Products at Trace Levels

2012 ◽  
Vol 84 (9) ◽  
pp. 3998-4005 ◽  
Author(s):  
Fabien Chainet ◽  
Jérémie Ponthus ◽  
Charles-Philippe Lienemann ◽  
Marion Courtiade ◽  
Olivier François Xavier Donard
Keyword(s):  
Mass Spectrometry ◽  
Fourier Transform ◽  
Molecular Characterization ◽  
Cyclotron Resonance ◽  
Petroleum Products ◽  
Mass Spectrometry Analysis ◽  
Ion Cyclotron Resonance ◽  
Spectrometry Analysis ◽  
Ion Cyclotron

Epitope Extraction Technique Using a Proteolytic Magnetic Reactor Combined with Fourier-Transform Ion Cyclotron Resonance Mass Spectrometry as a Tool for the Screening of Potential Vaccine Lead Peptides

2005 ◽  
Vol 11 (5) ◽  
pp. 489-495 ◽  
Author(s):  
Z. Bílková ◽  
R. Stefanescu ◽  
R. Cecal ◽  
L. Korecká ◽  
Š. Ouzká ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Fourier Transform ◽  
Cyclotron Resonance ◽  
Amyloid Peptide ◽  
Mass Spectrometry Analysis ◽  
Extraction Technique ◽  
Ion Cyclotron Resonance ◽  
Ion Cyclotron ◽  
Potential Vaccine ◽  
Immunoaffinity Isolation

Epitope extraction technique is based on the specific digestion of a target protein followed by immunoaffinity isolation of a specific recognition peptide. This technique, in combination with mass spectrometry, has been efficiently used for epitope identification. The major goal of this work was to utilize newly developed enzyme and immunoaffinity magnetic reactors for the epitope extraction procedure and confirm the efficiency of this improved system for epitope screening of proteins. Alginic acid-coated magnetite microparticles with immobilized TPCK-trypsin provided high working efficiency with low non-specific adsorption, digestion time in minutes and low frequency of missed cleavages. The sensitivity and specificity of tryptic fragmentation of the ß-amyloid-peptide Aβ (1–40) as a model polypeptide was confirmed by Fourier-transform ion cyclotron resonance mass spectrometry analysis. The Sepharose reactor or immunoaffinity magnetic reactors, both with anti-amyloid-β monoclonal antibodies, were used for specific isolation and identification of target peptides. In this way, the epitope extraction technique combined with mass spectrometric analysis is shown to be an excellent base for molecular screening of potential vaccine lead proteins.

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