Method for Determination of Peak Areas in Nonequilibrium Capillary Electrophoresis of Equilibrium Mixtures

2011 ◽  
Vol 83 (22) ◽  
pp. 8617-8622 ◽  
Author(s):  
Leonid T. Cherney ◽  
Mirzo Kanoatov ◽  
Sergey N. Krylov
Keyword(s):  
Capillary Electrophoresis ◽  
Peak Areas

Capillary electrophoresis facilitates determination of metal complex stoichiometry by Job’s method of continuous variation

2013 ◽  
Vol 10 (5) ◽  
pp. 409 ◽  
Author(s):  
Nathan E. Boland ◽  
Alan T. Stone
Keyword(s):  
Capillary Electrophoresis ◽  
Chelating Agent ◽  
Formation Constants ◽  
Continuous Variation ◽  
Spectroscopic Characteristics ◽  
Peak Areas ◽  
Method Of Continuous Variation ◽  
Job's Method ◽  
Metal Ligand

Environmental context Knowledge of metal-chelating agent speciation is integral to our ability to predict and interpret the behaviour of synthetic chelating agents in the environment. Capillary electrophoresis can be used to separate metal–ligand complexes with similar spectroscopic characteristics but different stoichiometries, thereby providing insight into metal–ligand speciation that is not possible by any other technique. Here, we demonstrate the utility of capillary electrophoresis for the determination of metal–ligand stoichiometries and evaluate its limitations. Abstract Job’s method of continuous variation is a traditional method used to determine the stoichiometry of metal–ligand complexes. The method is often applied to whole-sample absorbance measurements but its utility is limited when two or more complexes are present at significant concentrations and have similar absorption spectra. Here we employ capillary electrophoresis (CE), which separates complexes on the basis of charge and hydrodynamic radii, to extend the capabilities of Job’s method. Solutions containing nickel(II) and diethylenetriaminepentaacetic acid (DTPA) yield three CE peaks. Job’s method plot maxima, based on areas for each of the three CE peaks, coincide with nickel(II)-to-DTPA ratios of 1 : 1 and 1 : 2, which correspond to two complexes previously identified using whole-sample measurements, plus a ratio of 3 : 2, which corresponds to a previously unreported complex. We demonstrate how CE peak areas and electromigration times can be used to determine complex stoichiometries and formation constants. We discuss the strengths and weaknesses of Job’s Method coupled with CE and implications for speciation determination in environmentally relevant systems.

scholarly journals Development of a capillary electrophoresis method for the simultaneous determination of cephalosporins

2013 ◽  
Vol 78 (9) ◽  
pp. 1413-1423 ◽  
Author(s):  
Gabriel Hancu ◽  
Hajnal Kelemen ◽  
Aura Rusu ◽  
Árpád Gyéresi
Keyword(s):  
Capillary Electrophoresis ◽  
Simultaneous Determination ◽  
Sodium Dodecyl ◽  
Buffer Solution ◽  
Capillary Electrophoresis Method ◽  
Relative Standard ◽  
Peak Areas ◽  
Electrophoresis Method ◽  
Cephalosporin Antibiotics

A rapid and simple capillary electrophoresis method has been developed for the simultaneous determination of six extensively used cephalosporin antibiotics (cefaclor, cefadroxil, cefalexin, cefuroxim, ceftazidim, ceftriaxon). The determination of cephalosporins was performed at a pH 6.8, using a 25 mM phospate - 25 mM borate mixed buffer, + 25 kV voltage at a temperature of 25 ?C. We achieved a baseline separation in approximately 10 minutes. The separation resolution was increased by addition of an anionic surfactant, 50 mM sodium dodecyl sulfate, to the buffer solution. The proposed separation was evaluated on the basis of detection and quantification limits, effective electrophoretic mobility and relative standard deviation for migration times and peak areas.

Determination of Mutation of C-myc Oncogene in Gastric Cancer by Capillary Electrophoresis

2012 ◽  
Vol 39 (11) ◽  
pp. 1695-1700
Author(s):  
Xi-Hui XIE ◽  
Rong WANG ◽  
Zheng-Ping JIA ◽  
Hua XIE ◽  
Ai-Mei ZHAMG ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Capillary Electrophoresis ◽  
Myc Oncogene

Determination of gambogic acid inGambogeby non-aqueous capillary electrophoresis

2015 ◽  
Vol 33 (2) ◽  
pp. 152
Author(s):  
Wanlu OU ◽  
Yujuan LI ◽  
Dongdong SHI ◽  
Feng QU
Keyword(s):  
Capillary Electrophoresis ◽  
Gambogic Acid

scholarly journals Determination of Levels of NO-3, NO-2 and NH+4 Ions in Leaves of Various Plants by Capillary Electrophoresis

1996 ◽  
Vol 37 (6) ◽  
pp. 878-880 ◽  
Author(s):  
Y. Kawamura ◽  
M. Takahashi ◽  
G. Arimura ◽  
T. Isayama ◽  
K. Irifune ◽  
...  
Keyword(s):  
Capillary Electrophoresis
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