Nonesterified Fatty Acid Determination for Functional Lipidomics: Comprehensive Ultrahigh Performance Liquid Chromatography–Tandem Mass Spectrometry Quantitation, Qualification, and Parameter Prediction

2012 ◽  
Vol 84 (3) ◽  
pp. 1483-1490 ◽  
Author(s):  
Christian Hellmuth ◽  
Martina Weber ◽  
Berthold Koletzko ◽  
Wolfgang Peissner
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Fatty Acid ◽  
Nonesterified Fatty Acid ◽  
Tandem Mass ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass ◽  
Ultrahigh Performance Liquid Chromatography ◽  
Parameter Prediction ◽  
Acid Determination

202 Proteomic, metabolomic and fatty acid composition in lactating and non-lactating mares’ uterine fluids

2020 ◽  
Vol 32 (2) ◽  
pp. 229
Author(s):  
E. Derisoud ◽  
A. Hankele ◽  
L. Jouneau ◽  
C. Dubois ◽  
D. Rousseau-Ralliard ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Fatty Acid Composition ◽  
Liquid Chromatography ◽  
Fatty Acid ◽  
Tandem Mass Spectrometry ◽  
Acid Composition ◽  
Enrichment Score ◽  
Tandem Mass ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass

In the equine industry, horse breeders aim to produce one foal per mare per year. Thus, mares are bred while nursing. In high-yielding dairy cattle, concurrent lactation and conception may affect embryo quality, but effects on uterine fluid (UF) quality are unknown. No data are available in horses. The aim of our study was to analyse the effect of nursing on protein, metabolite and fatty acid (FA) compositions of UF at ~7.5 days post-ovulation. Anglo-Arab mares (multiparous (2.7±0.9 foals), 10.8±2.5 years old) that were either nursing (N; 105±12 days of lactation) or barren (B) were inseminated with the semen of the same stallion at induction of ovulation with human chorionic gonadotrophin. Ovulation was confirmed by ultrasound within 48h. At 7.4±0.7 days post-ovulation, UF was collected with a human tampon that was left for 10min in the uterus before embryo collection. Only mares for which no embryo was collected were selected (n=5 in both groups). Trypsin digestion followed by liquid chromatography-tandem mass spectrometry analysis was used for proteomic analysis, with subsequent characterisation with the PANTHER platform using an overrepresentation test (Fisher's exact type with false discovery rate correction) with the PANTHER pathway database. An untargeted approach based on liquid chromatography-tandem mass spectrometry was performed for metabolomics (normalisation to 50µg of protein), and data were analysed with the MS Peaks to Pathways tool of the MetaboAnalyst platform, using both the GSEA and mummichog algorithms (cut-off=0.05). Fatty acid composition (% of total FA) was analysed using gas chromatography. Differences between groups were analysed using a linear model with permutation using R software with the pgirmess package (P=0.05 for significance). Altogether, 2706 proteins with at least two peptides were identified in mares’ UF, with 164 being differentially expressed. Ubiquitin proteasome, involved in embryo-endometrium interactions, was the most enriched pathway in N mares (fold enrichment=15.12; P<0.0001). For metabolomics, ubiquinone biosynthesis [MetaFishNet, P=0.02; NES (Enrichment Score for the variable)/(mean of all Enrichment Score in all permutations in the dataset)=1.84] was enriched in N mares. N-Glycans, mainly guanosine diphosphate mannose (Kyoto Encyclopedia of Genes and Genomes, P=0.04; NES=−1.33) and leukotriene biosynthesis (Biocyc; P=0.006; NES=−1.31), were enriched in B mares with differentially expressed leukotrienes C4/D4. The proportion of saturated FA was higher in N vs. B mares (38.4±3.6% vs. 33.2±3.6%; P=0.03), probably due to increased palmitic (P=0.08) and stearic (P=0.08) acid proportions. In conclusion, pathways involved in uterine receptivity and inflammation seem to be enriched in B mares. Fatty acids that are readily available in the diet were more present in N mares, possibly because more elaborate FA are exported to the mammary gland for milk production. Nursing could thus modify the inflammatory response in the uterine environment at ~7.5 days post-ovulation and could affect reproductive efficiency in horses.

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