Sensitive and Simultaneous Detection of Cardiac Markers in Human Serum Using Surface Acoustic Wave Immunosensor

2011 ◽  
Vol 83 (22) ◽  
pp. 8629-8635 ◽  
Author(s):  
Joonhyung Lee ◽  
Youn-Suk Choi ◽  
Yeolho Lee ◽  
Hun Joo Lee ◽  
Jung Nam Lee ◽  
...  
Keyword(s):  
Acoustic Wave ◽  
Human Serum ◽  
Surface Acoustic Wave ◽  
Simultaneous Detection ◽  
Cardiac Markers

Rapid and Sensitive Detection of Cardiac Markers in Human Serum Using Surface Acoustic Wave Immunosensor

2012 ◽  
Vol 1415 ◽  
Author(s):  
Joonhyung Lee ◽  
Youn-Suk Choi ◽  
Yeolho Lee ◽  
Hun Joo Lee ◽  
Jung Nam Lee ◽  
...  
Keyword(s):  
Acoustic Wave ◽  
Human Serum ◽  
Surface Acoustic Wave ◽  
Troponin I ◽  
Signal Enhancement ◽  
Sandwich Immunoassay ◽  
Cardiac Markers ◽  
Saw Sensor ◽  
Enhancement Method ◽  
Capture Antibody

ABSTRACTWe present a rapid and sensitive surface acoustic wave (SAW) immunosensor that utilizes gold staining as a signal enhancement method. A sandwich immunoassay was performed on sensing area of the SAW sensor, which could specifically capture and detect cardiac markers (cardiac troponin I (cTnI), creatine kinase (CK)-MB, and myoglobin). The analytes in human serum were captured on gold nanoparticles (AuNPs) that were conjugated in advance with detection antibodies. Introduction of these complexes to the capture antibody-immobilized sensor surface resulted in a classic AuNP-based sandwich immunoassay format that has been used for signal amplification. In order to achieve further signal enhancement, a gold staining method was performed, which demonstrated that it is possible to obtain gold staining-mediated signal augmentation on a mass-sensitive device. The sensor response due to gold staining varied as a function of cardiac marker concentration.

scholarly journals Graphene Surface Acoustic Wave Sensor for Simultaneous Detection of Charge and Mass

ACS Sensors ◽  
2018 ◽  
Vol 3 (1) ◽  
pp. 200-204 ◽  
Author(s):  
Satoshi Okuda ◽  
Takao Ono ◽  
Yasushi Kanai ◽  
Takashi Ikuta ◽  
Masaaki Shimatani ◽  
...  
Keyword(s):  
Acoustic Wave ◽  
Surface Acoustic Wave ◽  
Simultaneous Detection ◽  
Graphene Surface ◽  
Acoustic Wave Sensor

scholarly journals Feasibility Study on Shear Horizontal Surface Acoustic Wave Sensors for Engine Oil Evaluation

Sensors ◽  
2020 ◽  
Vol 20 (8) ◽  
pp. 2184 ◽  
Author(s):  
Saya Kobayashi ◽  
Jun Kondoh
Keyword(s):  
Acoustic Wave ◽  
Surface Acoustic Wave ◽  
Simultaneous Detection ◽  
Horizontal Surface ◽  
Engine Oil ◽  
Used Engine Oil ◽  
Saw Sensor ◽  
Site Monitoring ◽  
Wave Sensors ◽  
Shear Horizontal

On site monitoring of engine oil is required. The features of a shear horizontal surface acoustic wave (SH-SAW) sensor include simultaneous detection of mechanical and electrical properties of liquids (such as viscosity, relative permittivity, and conductivity) and loaded mass on the sensor surface. In this paper, the used engine oil extracted from a motorbike was measured using the SH-SAW sensor. The degradation factors of the used engine oil were experimentally discussed. Especially, the influences of the particles in the engine oil, heating effect, and water contained in the engine oil were considered by comparing the differences between new and used engine oils. The results indicate that the influence of the water contained in the engine oil is the primary cause of the degradation of the used engine oil.

Kinetic assay of antitrypsin in human serum by a surface acoustic wave (SAW)-impedance sensor

1996 ◽  
Vol 356 (1) ◽  
pp. 96-97 ◽  
Author(s):  
Qingyun Cai ◽  
Wei Wei ◽  
Ronghui Wang ◽  
Lihua Nie ◽  
Shouzhuo Yao
Keyword(s):  
Acoustic Wave ◽  
Human Serum ◽  
Surface Acoustic Wave ◽  
Kinetic Assay ◽  
Impedance Sensor

Simultaneous Detection of Density and Viscosity Using Surface Acoustic Wave Liquid-Phase Sensors

2001 ◽  
Vol 40 (Part 1, No. 5B) ◽  
pp. 3713-3717 ◽  
Author(s):  
Jun Kondoh ◽  
Shinich Hayashi ◽  
Showko Shiokawa
Keyword(s):  
Liquid Phase ◽  
Acoustic Wave ◽  
Surface Acoustic Wave ◽  
Simultaneous Detection

scholarly journals Design of a Portable Orthogonal Surface Acoustic Wave Sensor System for Simultaneous Sensing and Removal of Nonspecifically Bound Proteins

Sensors ◽  
2019 ◽  
Vol 19 (18) ◽  
pp. 3876 ◽  
Author(s):  
Li ◽  
Bhethanabotla
Keyword(s):  
Acoustic Wave ◽  
Surface Acoustic Wave ◽  
Acoustic Waves ◽  
Point Of Care ◽  
Surface Acoustic Waves ◽  
Simultaneous Detection ◽  
Gain Control ◽  
Electronic System ◽  
Sensor System ◽  
Saw Sensor

One challenge for current surface acoustic wave (SAW) biosensors is reducing nonspecific adsorption. A device propagating Rayleigh and shear horizontal surface acoustic waves in orthogonal directions fabricated in ST quartz has the capability of achieving simultaneous detection and nonspecific binding (NSB) protein removal. Current measurement methods for a SAW sensor system based on this device require large-size and expensive equipment such as a vector network analyzer (VNA), signal generator, and frequency counter, which are not suitable for portable, especially point-of-care, applications. In this work, a portable platform based on a direct digital synthesizer (DDS) is investigated for the orthogonal SAW sensor, integrating signal synthesis, gain control, phase/amplitude measurement, and data processing in a small, portable electronic system. This prototype was verified for both stability and repeatability, and the results matched very well with VNA measurements. Finally, system performance in real-time sensing and NSB removal was evaluated.

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