Evaluation of a High-Throughput Online Solid Phase Extraction–Tandem Mass Spectrometry System for In Vivo Bioanalytical Studies

2011 ◽  
Vol 83 (21) ◽  
pp. 8259-8266 ◽  
Author(s):  
Wenying Jian ◽  
Michelle V. Romm ◽  
Richard W. Edom ◽  
Vaughn P. Miller ◽  
William A. LaMarr ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Solid Phase Extraction ◽  
High Throughput ◽  
Solid Phase ◽  
Tandem Mass ◽  
Phase Extraction ◽  
Online Solid Phase Extraction ◽  
Mass Spectrometry System

scholarly journals Development of a High-Throughput Online Solid-Phase Extraction/Tandem Mass Spectrometry Method for Cytochrome P450 Inhibition Screening

2010 ◽  
Vol 15 (4) ◽  
pp. 447-452 ◽  
Author(s):  
Kheng B. Lim ◽  
Can C. Özbal ◽  
Daniel B. Kassel
Keyword(s):  
Mass Spectrometry ◽  
Cytochrome P450 ◽  
Tandem Mass Spectrometry ◽  
Solid Phase Extraction ◽  
High Throughput ◽  
Solid Phase ◽  
Tandem Mass ◽  
Phase Extraction ◽  
Online Spe ◽  
Online Solid Phase Extraction

A high-throughput online solid-phase extraction/tandem mass spectrometry (online SPE/MS/MS) system has been developed to support rapid evaluation of drug discovery compounds for possible drug-drug interaction (DDI). Each compound is evaluated for its DDI potential by incubating over a range of 8 test concentrations and against a panel of 6 cytochrome P450 (CYP) enzymes, 1A2, 2C8, 2C9, 2C19, 2D6, and 3A4. Previously, a postassay pooling and a 2-min gradient LC/MS/MS method had been reported to increase sample throughput, allowing for a 96-well plate of samples to be analyzed in under 4 h. The development of a new online SPE/MS/MS system has reduced the analysis time to less than 15 min per 96-well plate, translating to a 15-fold time savings compared to the 2-min LC/MS/MS method. Sampling precision without internal standard correction ranged from 3.1% to 5.6% relative standard deviation, and the carryover was determined to be between 1.0% and 4.1%. One hundred twenty in-house compounds were assayed and pooled for analyses using both the online SPE/MS/MS and LC/MS/MS, and the correlation coefficients ranged from 0.89 to 1.13, when comparing the IC50 results obtained from the 2 approaches for each of the CYP enzymes.

Determination of F2-Isoprostanes in Urine by Online Solid Phase Extraction Coupled to Liquid Chromatography with Tandem Mass Spectrometry

2010 ◽  
Vol 58 (11) ◽  
pp. 6614-6620 ◽  
Author(s):  
Marsha L. Langhorst ◽  
Michael J. Hastings ◽  
Wallace H. Yokoyama ◽  
Shao-Ching Hung ◽  
Nicholas Cellar ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Solid Phase Extraction ◽  
Solid Phase ◽  
Tandem Mass ◽  
Phase Extraction ◽  
Online Solid Phase Extraction
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